anti-Rat (Rattus) IGF1R antibody for ELISA

Recommended IGF1R Antibody (supplied by: Log in to see )

Antigen
Insulin-Like Growth Factor 1 Receptor (IGF1R) Antibodies
  • CD221
  • IGFIR
  • IGFR
  • JTK13
  • IGFIRC
  • IGFR1
  • IGF-1R
  • IGF1R
  • DKFZp469I0618
  • igf-1r
  • xIGF-1R
  • xIGFR
  • xigf1r
  • A330103N21Rik
  • D930020L01
  • hyft
  • igf-ir
  • IGF-IRa
  • sb:eu1090
  • wu:fi20b10
  • insulin like growth factor 1 receptor
  • insulin-like growth factor 1 receptor
  • insulin-like growth factor I receptor
  • insulin like growth factor 1 receptor S homeolog
  • insulin-like growth factor 1a receptor
  • IGF1R
  • Igf1r
  • igf1r
  • LOC780503
  • LOC100136427
  • igf1r.S
  • igf1ra
Epitope
pTyr1346
49
45
42
40
29
28
26
22
18
16
16
15
15
15
13
13
10
9
8
7
7
7
7
6
6
5
5
4
4
4
4
4
4
4
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
580
333
311
26
20
19
18
16
13
12
5
3
2
2
2
1
Host
Rabbit
450
130
2
2
1
Clonality
Polyclonal
Conjugate
This IGF1R antibody is un-conjugated
21
19
19
18
12
12
8
8
8
8
8
8
8
8
8
8
6
3
3
3
3
2
2
2
1
1
1
1
1
1
1
Application
ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
405
186
157
110
104
94
84
51
39
15
8
6
5
3
2
2
2
2
1
1
1
1
1
Supplier
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Catalog No. ABIN6256637
$ 390.77
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
18.75568 ABIN3185133 ELISA IHC WB Rabbit IgG Ser1026 Log in to see Polyclonal 0
18.75568 ABIN3182037 ELISA IHC IP WB Rabbit IgG pTyr1161 Log in to see Polyclonal 0
18.75568 ABIN3182038 ELISA WB Rabbit IgG pTyr1165, pTyr1166 Log in to see Polyclonal 0
17.5 ABIN6255780 ELISA ICC IF IHC WB Rabbit IgG pTyr1165, pTyr1166 Log in to see Polyclonal 1
16 ABIN6256620 ELISA ICC IF IHC IP WB Rabbit IgG pTyr1161 Log in to see Polyclonal 0
15.755681 ABIN3185134 ELISA WB Rabbit IgG Ser52 Log in to see Polyclonal 0
15.755681 ABIN3182743 ELISA IF IHC WB Rabbit IgG pTyr1161 Log in to see Polyclonal 0
15.755681 ABIN3185141 ELISA IHC WB Rabbit IgG Ser528 Log in to see Polyclonal 0
14.5 ABIN5519001 ELISA IHC (p) WB Rabbit IgG AA 31-257 Log in to see Polyclonal 4
14.33835 ABIN1847558 IHC ELISA Rabbit pTyr1346 Log in to see Polyclonal 0
13 ABIN6269831 ELISA IHC WB Rabbit IgG pThr1163 Log in to see Polyclonal 0
13 ABIN6262491 ELISA ICC IF IHC WB Rabbit IgG Log in to see Polyclonal 0
13 ABIN1848190 IHC ELISA WB Rabbit Tyr1346 Log in to see Polyclonal 0
12.755681 ABIN6390601 ELISA WB Rabbit IgG pTyr1161 Log in to see Polyclonal 0
12.755681 ABIN3185140 ELISA WB Rabbit IgG Ser522 Log in to see Polyclonal 0
12.755681 ABIN3182901 ELISA IHC Rabbit IgG pTyr1346 Log in to see Polyclonal 0
12.755681 ABIN2141076 IHC ELISA Rabbit IgG pTyr1346 Log in to see Polyclonal 0
10 ABIN6262489 ELISA ICC IF WB Rabbit IgG Log in to see Polyclonal 0
10 ABIN6262490 ELISA ICC IF IHC WB Rabbit IgG Log in to see Polyclonal 0
7 ABIN1531870 ELISA IHC IP WB Rabbit IgG AA 1131-1180, pTyr1161 Log in to see Polyclonal 0

General

Antigen Insulin-Like Growth Factor 1 Receptor (IGF1R) Antibodies
Epitope pTyr1346
(49), (45), (42), (40), (29), (28), (26), (22), (18), (16), (16), (15), (15), (15), (13), (13), (10), (9), (8), (7), (7), (7), (7), (6), (6), (5), (5), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(580), (333), (311), (26), (20), (19), (18), (16), (13), (12), (5), (3), (2), (2), (2), (1)
Host Rabbit
(450), (130), (2), (2), (1)
Clonality
Polyclonal
Conjugate This IGF1R antibody is un-conjugated
(21), (19), (19), (18), (12), (12), (8), (8), (8), (8), (8), (8), (8), (8), (8), (8), (6), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Application ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
(405), (186), (157), (110), (104), (94), (84), (51), (39), (15), (8), (6), (5), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1)
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Product Details anti-IGF1R Antibody

Target Details IGF1R Application Details Handling Images
Specificity Phospho-IGF1R (Tyr1346) Antibody detects endogenous levels of IGF1R only when phosphorylated at Tyrosine 1346
Cross-Reactivity Human, Mouse (Murine), Rat (Rattus)
Purification The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Immunogen A synthesized peptide derived from human IGF1R around the phosphorylation site of Tyrosine 1346
Isotype IgG
Plasmids, Primers & others

Target Details IGF1R

Product Details anti-IGF1R Antibody Application Details Handling Images back to top
Antigen
Alternative Name IGF1R (IGF1R Antibody Abstract)
Background

Description: Receptor tyrosine kinase which mediates actions of insulin-like growth factor 1 (IGF1). Binds IGF1 with high affinity and IGF2 and insulin (INS) with a lower affinity. The activated IGF1R is involved in cell growth and survival control. IGF1R is crucial for tumor transformation and survival of malignant cell. Ligand binding activates the receptor kinase, leading to receptor autophosphorylation, and tyrosines phosphorylation of multiple substrates, that function as signaling adapter proteins including, the insulin-receptor substrates (IRS1/2), Shc and 14-3-3 proteins. Phosphorylation of IRSs proteins lead to the activation of two main signaling pathways: the PI3K-AKT/PKB pathway and the Ras-MAPK pathway. The result of activating the MAPK pathway is increased cellular proliferation, whereas activating the PI3K pathway inhibits apoptosis and stimulates protein synthesis. Phosphorylated IRS1 can activate the 85 kDa regulatory subunit of PI3K (PIK3R1), leading to activation of several downstream substrates, including protein AKT/PKB. AKT phosphorylation, in turn, enhances protein synthesis through mTOR activation and triggers the antiapoptotic effects of IGFIR through phosphorylation and inactivation of BAD. In parallel to PI3K-driven signaling, recruitment of Grb2/SOS by phosphorylated IRS1 or Shc leads to recruitment of Ras and activation of the ras-MAPK pathway. In addition to these two main signaling pathways IGF1R signals also through the Janus kinase/signal transducer and activator of transcription pathway (JAK/STAT). Phosphorylation of JAK proteins can lead to phosphorylation/activation of signal transducers and activators of transcription (STAT) proteins. In particular activation of STAT3, may be essential for the transforming activity of IGF1R. The JAK/STAT pathway activates gene transcription and may be responsible for the transforming activity. JNK kinases can also be activated by the IGF1R. IGF1 exerts inhibiting activities on JNK activation via phosphorylation and inhibition of MAP3K5/ASK1, which is able to directly associate with the IGF1R.

Gene: IGF1R

Molecular Weight kDa
Gene ID 3480
UniProt P08069
Pathways RTK Signaling, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Autophagy

Application Details

Product Details anti-IGF1R Antibody Target Details IGF1R Handling Images back to top
Application Notes IHC 1:50-1:200 WB 1:500-1:2000, IF/ICC 1:100-1:500
Restrictions For Research Use only

Handling

Product Details anti-IGF1R Antibody Target Details IGF1R Application Details Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer Rabbit IgG in phosphate buffered saline ,  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store at -20 °C.Stable for 12 months from date of receipt
Expiry Date 12 months

Images

Product Details anti-IGF1R Antibody Target Details IGF1R Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-Insulin-Like Growth Factor 1 Receptor (IGF1R) (pTyr1346) antibody (ABIN6256637) Western blot analysis of Phospho-IGF1R (Tyr1346) expression in various lysates
Immunofluorescence (fixed cells) (IF/ICC) image for anti-Insulin-Like Growth Factor 1 Receptor (IGF1R) (pTyr1346) antibody (ABIN6256637) ABIN6267336 staining 293 by IF/ICC. The sample were fixed with PFA and permeabilized ...
Immunohistochemistry (IHC) image for anti-Insulin-Like Growth Factor 1 Receptor (IGF1R) (pTyr1346) antibody (ABIN6256637) ABIN6267336 at 1/200 staining Human heart tissue sections by IHC-P. The tissue was fo...
Immunohistochemistry (IHC) image for anti-Insulin-Like Growth Factor 1 Receptor (IGF1R) (pTyr1346) antibody (ABIN6256637) ABIN6267336 at 1/200 staining Human heart tissue sections by IHC-P. The tissue was fo...
Immunohistochemistry (IHC) image for anti-Insulin-Like Growth Factor 1 Receptor (IGF1R) (pTyr1346) antibody (ABIN6256637) ABIN6267336 at 1/200 staining Human lung cancer tissue sections by IHC-P. The tissue ...
Immunohistochemistry (IHC) image for anti-Insulin-Like Growth Factor 1 Receptor (IGF1R) (pTyr1346) antibody (ABIN6256637) ABIN6267336 at 1/200 staining Rat lung tissue sections by IHC-P. The tissue was forma...
Immunohistochemistry (IHC) image for anti-Insulin-Like Growth Factor 1 Receptor (IGF1R) (pTyr1346) antibody (ABIN6256637) ABIN6267336 at 1/200 staining Rat lung tissue sections by IHC-P. The tissue was forma...
Immunohistochemistry (IHC) image for anti-Insulin-Like Growth Factor 1 Receptor (IGF1R) (pTyr1346) antibody (ABIN6256637) ABIN6267336 at 1/200 staining Rat ganstric tissue sections by IHC-P. The tissue was f...