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Ni-Charged Magnetic Beads

IP Nickel ions Magnetic particles 40 µm

(5 references)

Catalog No. ABIN400578

Datasheet as PDF

Application

Immunoprecipitation (IP)

Brand

MagBeads

Characteristics

Ni-charged MagBeads (8 mL 25% slurry) are average 40µm in size, superparamagnetic beads pre-charged with nickel ions and ready to use for quick and small-scale purification of His-tagged proteins. The beads are supplied as 25% slurry in phosphate buffered saline (PBS), pH 7.4, containing 20% ethanol. The Ni-Charged MagBeads have a binding capacity of 5 to 20 mg 6×His-tagged protein per 1 mL settled beads (e.g. 4 mL 25% slurry).The beads are compatible with magnetic separation-based high throughput applications. Proteins can be purified under non-denaturing conditions or in the presence of 8 M urea.

Bead Ligand

Nickel ions

Bead Matrix

Magnetic particles

Bead Size

40 µm
Magnetic Concanavalin A Beads (Agarose)
CUT&RUN, CUT&Tag, Sep Concanavalin A Magnetic Agarose beads 30 μm

Protein A Resin
Purif, AC Protein A Agarose beads 90 µm

Rho1D4 Agarose
Purif, Sep Rho1D4 40 µm

Glutathione Resin
Pull-Down Glutathione Agarose beads 90 µm

Protein G Resin
Purif, AC Protein G Agarose beads 90 µm

High Affinity Ni-Charged Resin
Purif Nickel ions Agarose beads 90 µm

MagSi-proteomics C18 beads
Purif, Sep, Conc, PD C18 alkyl group Magnetic Silica particles Bead size: 1.2 µm

Rho1D4 MagBeads
Pull-Down, Purif, Sep Rho1D4 25 µm

Magnetic ConA Beads for AAV/VLP Purification
Purif, Sep Concanavalin A Magnetic Agarose beads 30 μm

Protein L Resin
AC, Purif Protein L Agarose beads 90 µm

Restrictions

For Research Use only
Format

Liquid

Buffer

25% settled beads

Storage

4 °C

Storage Comment

Store at 4°C, do NOT freeze.

Expiry Date

12 months
Chen, Khajeh, Ju, Gupta, Stanley, Do, Heller, Aggarwal, Callaway, Bu: "Phosphatidylinositol 4,5-bisphosphate clusters the cell adhesion molecule CD44 and assembles a specific CD44-Ezrin heterocomplex, as revealed by small angle neutron scattering." in: The Journal of biological chemistry, Vol. 290, Issue 10, pp. 6639-52, (2015) (PubMed).

Dover, Barash, Burke, Hill, Detter, Arnon: "Arrangement of the Clostridium baratii F7 toxin gene cluster with identification of a ? factor that recognizes the botulinum toxin gene cluster promoters." in: PLoS ONE, Vol. 9, Issue 5, pp. e97983, (2014) (PubMed).

Dong, Tian, Gomez, Li: "Regulated proteolysis of the alternative sigma factor SigX in Streptococcus mutans: implication in the escape from competence." in: BMC microbiology, Vol. 14, pp. 183, (2014) (PubMed).

Leyme, Bourd-Boittin, Bonnier, Falconer, Arlot-Bonnemains, Théret: "Identification of ILK as a new partner of the ADAM12 disintegrin and metalloprotease in cell adhesion and survival." in: Molecular biology of the cell, Vol. 23, Issue 17, pp. 3461-72, (2012) (PubMed).

Wiltrout, Goodenbour, Fréchin, Pan: "Misacylation of tRNA with methionine in Saccharomyces cerevisiae." in: Nucleic acids research, Vol. 40, Issue 20, pp. 10494-506, (2012) (PubMed).

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