anti-Rabbit BRCA1 antibody for Western Blotting

Recommended BRCA1 Antibody (supplied by: Log in to see )

Antigen
Breast Cancer 1 (BRCA1) Antibodies
  • BRCAI
  • BRCC1
  • BROVCA1
  • IRIS
  • PNCA4
  • PPP1R53
  • PSCP
  • RNF53
  • iris
  • pscp
  • brcai
  • brcc1
  • rnf53
  • brca1
  • BRCA1
  • LOC100224649
  • BRCA1, DNA repair associated
  • breast cancer 1
  • breast cancer 1, early onset
  • breast cancer 1 L homeolog
  • BRCA1
  • brca1
  • LOC755086
  • Brca1
  • brca1.L
Epitope
N-Term
73
57
41
36
30
24
23
20
19
18
17
16
15
15
11
10
9
7
6
5
5
5
4
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Dog (Canine), Horse (Equine), Human, Rabbit, Rat (Rattus)
657
84
62
7
1
1
Host
Rabbit
414
253
4
3
Clonality
Polyclonal
Conjugate
This BRCA1 antibody is un-conjugated
28
22
21
12
12
10
10
8
8
8
7
7
7
7
7
7
5
5
5
5
5
5
5
5
5
4
4
4
4
2
2
2
2
2
2
2
2
2
2
2
2
2
Application
Western Blotting (WB)
393
228
207
165
125
117
113
86
68
22
21
21
12
6
5
4
3
2
2
1
1
Options
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Available images

Catalog No. ABIN2779333
$ 319.00
Plus shipping costs $45.00

General

Antigen Breast Cancer 1 (BRCA1) Antibodies
Epitope N-Term
(73), (57), (41), (36), (30), (24), (23), (20), (19), (18), (17), (16), (15), (15), (11), (10), (9), (7), (6), (5), (5), (5), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Dog (Canine), Horse (Equine), Human, Rabbit, Rat (Rattus)
(657), (84), (62), (7), (1), (1)
Host Rabbit
(414), (253), (4), (3)
Clonality
Polyclonal
Conjugate This BRCA1 antibody is un-conjugated
(28), (22), (21), (12), (12), (10), (10), (8), (8), (8), (7), (7), (7), (7), (7), (7), (5), (5), (5), (5), (5), (5), (5), (5), (5), (4), (4), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application Western Blotting (WB)
(393), (228), (207), (165), (125), (117), (113), (86), (68), (22), (21), (21), (12), (6), (5), (4), (3), (2), (2), (1), (1)
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Product Details anti-BRCA1 Antibody

Target Details BRCA1 Application Details Handling Images
Blocking Peptide Blocking peptide for this product available: ABIN973870
Sequence DLSALRVEEV QNVINAMQKI LECPICLELI KEPVSTKCDH IFCKFCMLKL
Predicted Reactivity Dog: 92%, Horse: 85%, Human: 100%, Rabbit: 85%, Rat: 77%
Characteristics This is a rabbit polyclonal antibody against BRCA1. It was validated on Western Blot.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the N terminal region of human BRCA1
Plasmids, Primers & others

Target Details BRCA1

Product Details anti-BRCA1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name BRCA1 (BRCA1 Antibody Abstract)
Background The BRCA1-BARD1 heterodimer coordinates a diverse range of cellular pathways such as DNA damage repair, ubiquitination and transcriptional regulation to maintain genomic stability. BRCA1 acts by mediating ubiquitin E3 ligase activity that is required for its tumor suppressor function. BRCA1 plays a central role in DNA repair by facilitating cellular response to DNA repair. BRCA1 is required for appropriate cell cycle arrests after ionizing irradiation in both the S-phase and the G2 phase of the cell cycle. BRCA1 is involved in transcriptional regulation of P21 in response to DNA damage. BRCA1 is also required for FANCD2 targeting to sites of DNA damage.It may function as a transcriptional regulator. BRCA1 inhibits lipid synthesis by binding to inactive phosphorylated ACACA and preventing its dephosphorylation.
Alias Symbols: BRCAI, BRCC1, IRIS, PSCP, RNF53, PNCA4, BROVCA1, PPP1R53
Protein Interaction Partner: RAD50, MED17, RAD51, YAP3, HDA3, SSN3, BEM4, GYP5, YAF9, HDAC2, MSH6, BAP1, SUPT5H, POLR2A, NPR1, NUP53, MET18, CDC21, MLP1, CTK1, MSH2, BACH1, DAN1, TMA22, BBC1, RPB4, SPT4, YGR053C, ATE1, YBP2, SLI15, RAD16, XRS2, RAD55, BUR2, BRIP1, ESR1, BECN1, HIST1H
Protein Size: 680
Molecular Weight 76 kDa
Gene ID 672
NCBI Accession NM_007298, NP_009229
UniProt P38398
Pathways Cell Division Cycle, DNA Damage Repair, Intracellular Steroid Hormone Receptor Signaling Pathway, Positive Regulation of Response to DNA Damage Stimulus

Application Details

Product Details anti-BRCA1 Antibody Target Details BRCA1 Handling Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 680 AA

Restrictions For Research Use only

Handling

Product Details anti-BRCA1 Antibody Target Details BRCA1 Application Details Images back to top
Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Images

Product Details anti-BRCA1 Antibody Target Details BRCA1 Application Details Handling back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-Breast Cancer 1 (BRCA1) (N-Term) antibody (ABIN2779333) Rabbit Anti-BRCA1 Antibody Catalog Number: ARP30434_P050 Formalin Fixed Paraffin Embe...
Western Blotting (WB) image for anti-Breast Cancer 1 (BRCA1) (N-Term) antibody (ABIN2779333) WB Suggested Anti-BRCA1 Antibody Titration: 0.2-1 ug/ml ELISA Titer: 1:1562500 Positi...