anti-Human CD180 antibody for Immunoprecipitation

Recommended CD180 Antibody (supplied by: Log in to see )

Antigen
CD180 Molecule (CD180) Antibodies
  • Ly78
  • CD180
  • LY64
  • RP105
  • F630107B15
  • CD180 molecule
  • CD180 antigen
  • Cd180
  • CD180
Reactivity
Human
118
36
3
2
Host
Mouse
70
50
19
Clonality
Monoclonal
Conjugate
This CD180 antibody is conjugated to PE
12
12
11
5
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Functional Studies (Func), Flow Cytometry (FACS), Immunoprecipitation (IP)
85
50
42
34
30
26
19
12
5
4
3
2
1
Options
Supplier
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Available images

Catalog No. ABIN638438
$ 497.45
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
16.905727 ABIN2749219 Func FACS IP Mouse IgG1 Log in to see G28-8 6
16.905727 ABIN559680 Func FACS IP Mouse IgG1 Log in to see G28-8 6
16.905727 ABIN2749113 Func FACS IP APC Mouse IgG1 Log in to see G28-8 6
16.905727 ABIN2191954 Func IHC (fro) FACS IP Mouse Log in to see MHR73 2
4 ABIN476152 FACS IP Mouse IgG1, kappa Log in to see MHR73-11 0
4 ABIN205467 FACS IHC IHC (fro) IP Mouse IgG1 Log in to see MHR73-11 0
4 ABIN3036828 FACS IP PE Mouse IgG1 Log in to see 0
4 ABIN3036827 FACS IP APC Mouse IgG1 Log in to see 0
4 ABIN2663107 FACS IP IHC WB PE Mouse IgG1 kappa Log in to see MHR73-11 2
1 ABIN614758 Func FACS IP Mouse IgG1 Log in to see G28-8 1
1 ABIN600958 FACS IHC IHC (fro) IP FITC Mouse IgG1 Log in to see MHR73 0
1 ABIN600957 FACS IHC IHC (fro) IP Mouse IgG1 Log in to see MHR73 0
1 ABIN1959973 Func FACS IP Mouse IgG1 Log in to see G28-8 0
1 ABIN225884 ELISA FACS IHC IHC (fro) IP Biotin Mouse IgG1 Log in to see 200-1 0
1 ABIN600960 FACS Func IHC IHC (fro) IHC (p) IP Mouse IgG1 Log in to see 0
1 ABIN1105954 Func IHC (fro) IHC (p) IP Mouse IgG1 Log in to see MHR73 2
1 ABIN1105958 Func IHC (fro) IP FITC Mouse IgG1 Log in to see MHR73 2
1 ABIN1818013 FACS IP IHC Mouse IgG1 Log in to see MHR73 0
1 ABIN2223520 FACS IP IHC FITC Mouse IgG1 Log in to see MHR73 0
1 ABIN2223519 FACS IP IHC Mouse IgG1 Log in to see MHR73 0

General

Antigen CD180 Molecule (CD180) Antibodies
Reactivity Human
(118), (36), (3), (2)
Host Mouse
(70), (50), (19)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD180 antibody is conjugated to PE
(12), (12), (11), (5), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Functional Studies (Func), Flow Cytometry (FACS), Immunoprecipitation (IP)
(85), (50), (42), (34), (30), (26), (19), (12), (5), (4), (3), (2), (1)
Pubmed 6 references available
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Product Details anti-CD180 Antibody

Target Details CD180 Application Details Handling References for anti-CD180 antibody (ABIN638438) Images
Specificity The mouse monoclonal antibody G28-8 recognizes CD180, a 95-105 kDa TLR-like glycoprotein expressed on peripheral blood monocytes and dendritic cells, mantle zone B cells and marginal zone B cells, but very weakly on germinal center B cells.
Cross-Reactivity (Details) Human
Immunogen Human tonsillar B cells
Clone G28-8
Isotype IgG1
Plasmids, Primers & others

Target Details CD180

Product Details anti-CD180 Antibody Application Details Handling References for anti-CD180 antibody (ABIN638438) Images back to top
Antigen
Alternative Name CD180 (CD180 Antibody Abstract)
Background CD180, also known as RP105 (or Bgp95, LY64) is a type I membrane glycoprotein of Toll-like receptor (TLR) family. Its cytoplasmic tail is short and unlike the TLRs, it lacks the TIR domain. CD180 expression is dependent on the coexpression of its helper molecule, MD-1 , and mirrors that of TLR4 on antigen-presenting cells. CD180 regulates recognition of LPS and signaling in B cells, via interacting directly with the TLR4 signaling complex, inhibiting its ability to bind microbial ligands. Ligation of CD180 by monoclonal antibodies leads to B cell activation, upregulation of CD80/CD86, and increase in cell size.
Gene ID 4064
Pathways Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades

Application Details

Product Details anti-CD180 Antibody Target Details CD180 Handling References for anti-CD180 antibody (ABIN638438) Images back to top
Application Notes The reagent is designed for Flow Cytometry analysis of human blood cells using 20 μ,l reagent / 100 μ,l of whole blood or 106 cells in a suspension.The content of a vial (2 ml) is sufficient for 100 tests.
Comment

The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.

Restrictions For Research Use only

Handling

Product Details anti-CD180 Antibody Target Details CD180 Application Details References for anti-CD180 antibody (ABIN638438) Images back to top
Reconstitution No reconstitution is necessary.
Buffer The reagent is provided in stabilizing phosphate buffered saline (PBS) solution containing 15 mM sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Avoid prolonged exposure to light.
Storage 4 °C
Storage Comment Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.

References for anti-CD180 antibody (ABIN638438)

Product Details anti-CD180 Antibody Target Details CD180 Application Details Handling Images back to top
Background publications

Divanovic, Trompette, Petiniot, Allen, Flick, Belkaid, Madan, Haky, Karp: "Regulation of TLR4 signaling and the host interface with pathogens and danger: the role of RP105." in: Journal of leukocyte biology, Vol. 82, Issue 2, pp. 265-71, 2007

Yazawa, Fujimoto, Sato, Miyake, Asano, Nagai, Takeuchi, Takeda, Okochi, Akira, Tedder, Tamaki: "CD19 regulates innate immunity by the toll-like receptor RP105 signaling in B lymphocytes." in: Blood, Vol. 102, Issue 4, pp. 1374-80, 2003

Kikuchi, Koarada, Tada, Ushiyama, Morito, Suzuki, Ohta, Horiuchi, Miyake, Nagasawa: "Difference in B cell activation between dermatomyositis and polymyositis: analysis of the expression of RP105 on peripheral blood B cells." in: Annals of the rheumatic diseases, Vol. 60, Issue 12, pp. 1137-40, 2001

Product cited in:

Olson, Graves, Shu, Ryan, Clark: "Caspase activity is required for stimulated B lymphocytes to enter the cell cycle." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 170, Issue 12, pp. 6065-72, 2003

Clark, Shu, Lüscher, Draves, Banchereau, Ledbetter, Valentine: "Activation of human B cells. Comparison of the signal transduced by IL-4 to four different competence signals." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 143, Issue 12, pp. 3873-80, 1990

Valentine, Clark, Shu, Norris, Ledbetter: "Antibody to a novel 95-kDa surface glycoprotein on human B cells induces calcium mobilization and B cell activation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 140, Issue 12, pp. 4071-8, 1988

Images

Product Details anti-CD180 Antibody Target Details CD180 Application Details Handling References for anti-CD180 antibody (ABIN638438) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-CD180 Molecule (CD180) antibody (PE) (ABIN638438) Surface staining of human peripheral blood cells with anti-CD180 (G28-8) PE.