anti-Human C3 antibody for Enzyme Immunoassay

Recommended C3 Antibody (supplied by: Log in to see )

Antigen
Complement Component 3 (C3) Antibodies
  • AHUS5
  • ARMD9
  • ASP
  • C3a
  • C3b
  • CPAMD1
  • AI255234
  • HSE-MSF
  • Plp
  • C3
  • C3-1
  • sb:cb26
  • si:dkey-76b14.4
  • wu:fi34b03
  • wu:fj86e11
  • C3d
  • asp
  • cpamd1
  • c3
  • complement C3
  • complement component 3
  • complement component c3a, duplicate 1
  • complement component 3 L homeolog
  • complement component C3
  • complement C3-like
  • C3
  • c3a.1
  • c3.L
  • LOC443475
  • c3
  • c3-1
  • LOC100060539
Reactivity
Human
296
80
72
16
15
9
7
6
5
4
3
1
Host
Mouse
172
133
98
27
14
8
Clonality
Monoclonal
Conjugate
This C3 antibody is un-conjugated
38
37
23
7
7
6
4
4
4
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
230
206
105
78
68
61
56
51
44
39
38
32
16
11
5
5
5
2
2
2
1
1
1
1
1
1
1
1
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Catalog No. ABIN317012
$ 753.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN181105 EIA WB Mouse IgG1 Log in to see 01-10-21 2
1 ABIN784157 EIA IHC (fro) IHC (p) Sheep IgG Log in to see Polyclonal 0
1 ABIN1106781 EIA WB Mouse IgG1 C-Term Log in to see 2898 2
1 ABIN181129 EIA FACS IHC (fro) Mouse IgG1 Log in to see 053A-514-3-1-4 2
1 ABIN568572 DB EIA ID IHC (fro) IF IP WB HRP Goat IgG Log in to see Polyclonal 0
1 ABIN568570 DB EIA ID IHC (fro) IF IP WB Biotin Goat IgG Log in to see Polyclonal 0
1 ABIN1107627 EIA IP Rat IgG1 Log in to see 9 5
1 ABIN568571 EIA ID IHC (fro) IF IP FITC Goat IgG Log in to see Polyclonal 0
1 ABIN568574 EIA ID IHC (fro) IF IP TRITC Goat IgG Log in to see Polyclonal 0
1 ABIN1106783 EIA WB Biotin Mouse IgG1 Log in to see 474 4
1 ABIN181106 EIA FACS IHC (fro) WB Mouse IgG1 Log in to see 10-02A 1
1 ABIN181107 EIA FACS IHC (fro) WB Mouse IgG1 Log in to see 053A-1149-3-1-4 (1003) 2
1 ABIN1106778 EIA WB Mouse IgG1 Log in to see 474 4
1 ABIN111064 EIA IHC (fro) WB Mouse IgG2b Log in to see H13 3
1 ABIN1106782 EIA WB Mouse IgG1 C-Term Log in to see 755 1
1 ABIN112153 EIA IHC (fro) WB Mouse IgG1 Log in to see H206 1
1 ABIN951635 EIA FACS WB Chicken Ig Fraction Log in to see Polyclonal 1
1 ABIN113339 EIA Chicken C-Term Log in to see Polyclonal 0
1 ABIN113306 EIA WB HRP Sheep Log in to see Polyclonal 1
1 ABIN113307 EIA ID Sheep Log in to see Polyclonal 0

General

Antigen Complement Component 3 (C3) Antibodies
Reactivity Human
(296), (80), (72), (16), (15), (9), (7), (6), (5), (4), (3), (1)
Host Mouse
(172), (133), (98), (27), (14), (8)
Clonality (Clone)
Monoclonal   ( )
Conjugate This C3 antibody is un-conjugated
(38), (37), (23), (7), (7), (6), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
(230), (206), (105), (78), (68), (61), (56), (51), (44), (39), (38), (32), (16), (11), (5), (5), (5), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-C3 Antibody

Target Details C3 Application Details Handling References for anti-C3 antibody (ABIN317012) Images
Specificity AM05608PU-N Recognises 42 kDa inactive complement component 3b (iC3b) neoantigen present in blood serum.
Cross-Reactivity (Details) Species reactivity (tested):Human.
Characteristics Synonyms: CPAMD1, Complement component 3, C3 and PZP-like alpha-2-macroglobulindomain-containing protein 1
Purification Purified IgG prepared by affinity chromatography on Protein A
Immunogen Purified Human iC3b
Clone 013III-1-16
Isotype IgG2b
Plasmids, Primers & others

Target Details C3

Product Details anti-C3 Antibody Application Details Handling References for anti-C3 antibody (ABIN317012) Images back to top
Antigen
Alternative Name Complement C3 (C3 Antibody Abstract)
Background Cleavage of C3b into iC3b by the protease factor I, in the presence of cofactors, changes the structure and resulting binding properties, preventing binding by complement factor B and properdin. This prevents further progression of the complement pathway, providing another mechanism by which the complement pathway may be regulated. iC3b is thought to play a role in inducing tolerance, binding complement receptor type 3 on antigen presenting cells and stimulating them to produce transforming growth factor beta2 and interleukin-10. It is thought to stimulate B-cells via the CD21 receptor providing a link between the innate and adaptive immune responses. Additionally iC3b acts as an opsonin which is recognised by complement receptor 3 expressed on leukocytes or complement receptor of the immunoglobulin superfamily (CRIg) on Kupffer cells, stimulating phagocytosis of the tagged cells. iC3b levels are often elevated in diseases such as Systemic Lupus Erythematosis and Rheumatoid arthritis.Synonyms: C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1, CPAMD1, Complement component 3
Gene ID 718
UniProt P01024
Pathways Complement System, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Regulation of G-Protein Coupled Receptor Protein Signaling

Application Details

Product Details anti-C3 Antibody Target Details C3 Handling References for anti-C3 antibody (ABIN317012) Images back to top
Application Notes ELISA. Western blot. Flow cytometry. Immunohistochemistry on Frozen Sections. Histology Positive Control Tissue: Kidney from post streptoccal glomerulonephritispatients.
Restrictions For Research Use only

Handling

Product Details anti-C3 Antibody Target Details C3 Application Details References for anti-C3 antibody (ABIN317012) Images back to top
Concentration 1.0 mg/mL
Buffer Borate buffered saline pH 8.2-8.4, 0.09 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Expiry Date 12 months

References for anti-C3 antibody (ABIN317012)

Product Details anti-C3 Antibody Target Details C3 Application Details Handling Images back to top
Background publications

Janssen, Gros: "Structural insights into the central complement component C3." in: Molecular immunology, Vol. 44, Issue 1-3, pp. 3-10, 2006

Bergmann-Leitner, Leitner, Tsokos: "Complement 3d: from molecular adjuvant to target of immune escape mechanisms." in: Clinical immunology (Orlando, Fla.), Vol. 121, Issue 2, pp. 177-85, 2006

Sohn, Bora, Suk, Molina, Kaplan, Bora: "Tolerance is dependent on complement C3 fragment iC3b binding to antigen-presenting cells." in: Nature medicine, Vol. 9, Issue 2, pp. 206-12, 2003