|Antigen||Complement Component 5 (C5) Antibodies|
|Reactivity||Cow (Bovine), Goat, Human, Pig (Porcine), Primate Alternatives|
Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
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|Specificity||HCC 5b.1 (neo) allows detection of C5b-9 complexes via activated C5, especially in different forms of glomerulonephritis. Does not react with native C5! Polypeptide Reacting: Mr 200 000 polypeptide of SDS denaturated complement component C5 and C5b of Mr 185 000, after C5b activation, an Mr 60 000 cleavage product also reacts (weakly). Disorders Specifically Detected: Detection of C5b-9 complexes via activated C5, whereas native C5 reacts with antibody HCC 5.1.|
|Cross-Reactivity (Details)||Species reactivity (tested):Human, Primates, Bovine, Pig, Goat.|
|Purification||Affinity Chromatography on Protein A|
|Immunogen||Activated Human Complement Component C5|
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|Alternative Name||Complement C5 (C5 Antibody Abstract)|
|Background||C5 is synthesised in the liver as a single polypeptide chain. Before secretion the molecule is glycosylated and secreted into plasma as a 190 kDa glycoprotein consisting of a disulphide linked alpha-chain (111 kDa) and beta-chain (75 kDa). C5 precursor is first processed by the removal of 4 basic residues, forming two chains, beta and alpha, linked by a disulfide bond. C5 convertase activates C5 by cleaving the alpha chain, releasing C5a anaphylatoxin and generating C5b (beta chain + alpha' chain).Synonyms: CPAMD4, Complement Component 5|
|Pathways||Complement System, Carbohydrate Homeostasis|
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ELISA. Immunoblotting (Western blot). Cytological material. Immunohistochemistry on Frozen Sections (1/10). Incubation time: 1h at RT.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
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|Reconstitution||Restore with 1 mL distilled water.|
|Handling Advice||This product is photosensitive and should be protected from light|
|Storage Comment||Prior to and following reconstitution store the antibody undiluted at 2-8 °C. DO NOT FREEZE!|