anti-Cow (Bovine) Complement Component 5 antibody for Enzyme Immunoassay

Recommended Complement Component 5 Antibody (supplied by: Log in to see )

Antigen
Complement Component 5 (C5) Antibodies
  • C5
  • LOC565774
  • C5a
  • C5b
  • CPAMD4
  • Hc
  • RGD1561905
  • He
  • complement C5
  • complement component 5
  • hemolytic complement
  • C5
  • c5
  • Hc
Reactivity
Cow (Bovine), Goat, Human, Pig (Porcine), Primate
175
22
16
9
7
1
1
1
Host
Mouse
105
86
11
6
Clonality
Monoclonal
Conjugate
Un-conjugated
16
14
8
6
6
4
4
4
3
3
3
3
3
3
3
3
3
2
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
133
113
29
23
19
15
13
10
10
9
8
7
6
5
4
3
2
2
2
2
1
1
1
1
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Catalog No. ABIN111066
$ 368.50
Plus shipping costs $45.00

General

Antigen Complement Component 5 (C5) Antibodies
Reactivity Cow (Bovine), Goat, Human, Pig (Porcine), Primate
(175), (22), (16), (9), (7), (1), (1), (1)
Host Mouse
(105), (86), (11), (6)
Clonality (Clone)
Monoclonal   ( )
Conjugate Un-conjugated
(16), (14), (8), (6), (6), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
(133), (113), (29), (23), (19), (15), (13), (10), (10), (9), (8), (7), (6), (5), (4), (3), (2), (2), (2), (2), (1), (1), (1), (1)
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Product details

Target Details Application Details Handling Images
Specificity HCC 5b.1 (neo) allows detection of C5b-9 complexes via activated C5, especially in different forms of glomerulonephritis. Does not react with native C5! Polypeptide Reacting: Mr 200 000 polypeptide of SDS denaturated complement component C5 and C5b of Mr 185 000, after C5b activation, an Mr 60 000 cleavage product also reacts (weakly). Disorders Specifically Detected: Detection of C5b-9 complexes via activated C5, whereas native C5 reacts with antibody HCC 5.1.
Cross-Reactivity (Details) Species reactivity (tested):Human, Primates, Bovine, Pig, Goat.
Purification Affinity Chromatography on Protein A
Immunogen Activated Human Complement Component C5
Clone HCC5b-1 (neo)
Isotype IgG1

Target Details

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Antigen
Alternative Name Complement C5 (C5 Antibody Abstract)
Background C5 is synthesised in the liver as a single polypeptide chain. Before secretion the molecule is glycosylated and secreted into plasma as a 190 kDa glycoprotein consisting of a disulphide linked alpha-chain (111 kDa) and beta-chain (75 kDa). C5 precursor is first processed by the removal of 4 basic residues, forming two chains, beta and alpha, linked by a disulfide bond. C5 convertase activates C5 by cleaving the alpha chain, releasing C5a anaphylatoxin and generating C5b (beta chain + alpha' chain).Synonyms: CPAMD4, Complement Component 5
Gene ID 727
NCBI Accession NP_001726
UniProt P01031
Pathways Complement System, Carbohydrate Homeostasis

Application Details

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Application Notes ELISA. Immunoblotting (Western blot). Cytological material. Immunohistochemistry on Frozen Sections (1/10). Incubation time: 1h at RT.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

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Reconstitution Restore with 1 mL distilled water.
Handling Advice This product is photosensitive and should be protected from light
Storage 4 °C
Storage Comment Prior to and following reconstitution store the antibody undiluted at 2-8 °C. DO NOT FREEZE!