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|Conjugate||This CD59 antibody is un-conjugated Alternatives|
Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
|6 references available|
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Product Details anti-CD59 AntibodyTarget Details CD59 Application Details Handling References for anti-CD59 antibody (ABIN119472) Images
|Specificity||This antibody recognises the CD59 cell surface antigen. Clone YTH53.1 has been shown to block the normal function of CD59.|
|Purification||Affinity Chromatography on Protein G|
|Immunogen||Human Peripheral Blood T cells. Spleen cells from an immunised DA rat were fused with cells of the Y3/Ag1.2.3 rat myeloma line.|
Target Details CD59Product Details anti-CD59 Antibody Application Details Handling References for anti-CD59 antibody (ABIN119472) Images back to top
|Alternative Name||CD59 (CD59 Antibody Abstract)|
|Background||CD59 is an 18-20kD GPI linked glycoprotein broadly expressed by human leucocytes and erythrocytes. CD59 is expressed by erythrocytes, and therefore staining in whole blood systems requires a higher concentration of antibody.Synonyms: 20 kDa homologous restriction factor, HRF-20, HRF20, MAC-IP, MAC-inhibitory protein, MACIF, MEM43 antigen, MIC11, MIN1, MIN2, MIN3, MIRL, MSK21, Membrane attack complex inhibition factor, Membrane inhibitor of reactive lysis, Protectin|
Application DetailsProduct Details anti-CD59 Antibody Target Details CD59 Handling References for anti-CD59 antibody (ABIN119472) Images back to top
Western Blot. Flow cytometry: 1/50 - 1/100, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Immunohistochemistry on frozen sections. Immunohistochemistry on paraffin sections: 1/5 - 1/10, This product requires antigen retrieval using heat treatment prior to staining of paraffin sections, Sodium citrate buffer pH 6.0 is recommended for this purpose. Recommended Positive Control: Placenta. Removal of sodium azide is recommended prior to use in functional assays.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-CD59 Antibody Target Details CD59 Application Details References for anti-CD59 antibody (ABIN119472) Images back to top
|Buffer||PBS containing 0.09 % Sodium Azide as preservative.|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.|
References for anti-CD59 antibody (ABIN119472)Product Details anti-CD59 Antibody Target Details CD59 Application Details Handling Images back to top
Yang, Tyrrell, Han, Jaffe: "Expression and modulation of RPE cell membrane complement regulatory proteins." in: Investigative ophthalmology & visual science, Vol. 50, Issue 7, pp. 3473-81, 2009
Ajona, Hsu, Corrales, Montuenga, Pio: "Down-regulation of human complement factor H sensitizes non-small cell lung cancer cells to complement attack and reduces in vivo tumor growth." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 178, Issue 9, pp. 5991-8, 2007
Davies, Wilson, Bramley, Willers, Van Heyningen, Bickmore, Lachmann: "Identification of MIC 11 antigen as an epitope of the CD59 molecule." in: Immunology, Vol. 85, Issue 2, pp. 220-7, 1995
Walsh, Tone, Waldmann: "Transfection of human CD59 complementary DNA into rat cells confers resistance to human complement." in: European journal of immunology, Vol. 21, Issue 3, pp. 847-50, 1991
Davies, Simmons, Hale, Harrison, Tighe, Lachmann, Waldmann: "CD59, an LY-6-like protein expressed in human lymphoid cells, regulates the action of the complement membrane attack complex on homologous cells." in: The Journal of experimental medicine, Vol. 170, Issue 3, pp. 637-54, 1989
Gilman, Wiggs, Chamberlin: "Nucleotide sequences of two Bacillus subtilis promoters used by Bacillus subtilis sigma-28 RNA polymerase." in: Nucleic acids research, Vol. 9, Issue 22, pp. 5991-6000, 1982
ImagesProduct Details anti-CD59 Antibody Target Details CD59 Application Details Handling References for anti-CD59 antibody (ABIN119472) back to top