anti-Pig (Porcine) CD59 antibody for Functional Studies

Recommended CD59 Antibody (supplied by: Log in to see )

Antigen
CD59 Antibodies
  • 16.3A5
  • 1F5
  • EJ16
  • EJ30
  • EL32
  • G344
  • HRF-20
  • HRF20
  • MAC-IP
  • MACIF
  • MEM43
  • MIC11
  • MIN1
  • MIN2
  • MIN3
  • MIRL
  • MSK21
  • p18-20
  • AA987121
  • Cd59
  • protectin
  • Cd59a
  • Cd59b
  • MACIP
  • CD59 molecule (CD59 blood group)
  • CD59a antigen
  • CD59 molecule
  • CD59
  • Cd59a
  • Cd59
Reactivity
Human, Pig (Porcine)
510
44
39
22
17
14
9
8
2
2
1
1
Host
Mouse
410
124
41
7
1
Clonality
Monoclonal
Conjugate
This CD59 antibody is un-conjugated
63
53
44
22
14
12
12
12
10
10
10
10
10
10
10
6
6
5
1
1
1
1
1
1
1
1
1
1
1
1
Application
Functional Studies (Func), Flow Cytometry (FACS)
427
208
198
127
114
93
78
47
46
24
13
10
9
4
3
2
1
1
1
1
1
1
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Catalog No. ABIN94204
$ 265.65
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN120694 Func FACS Mouse IgM Log in to see MEM-129 1
1 ABIN269160 Func FACS Mouse IgM Log in to see MEM-129 0
1 ABIN4294909 FACS Func PerCP Mouse IgM Log in to see MEM-129 0
1 ABIN3199956 FACS Func Mouse IgM Log in to see MEM-129 0
1 ABIN3037467 Func FACS Mouse IgM Log in to see 0

General

Antigen CD59 Antibodies
Reactivity Human, Pig (Porcine)
(510), (44), (39), (22), (17), (14), (9), (8), (2), (2), (1), (1)
Host Mouse
(410), (124), (41), (7), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD59 antibody is un-conjugated
(63), (53), (44), (22), (14), (12), (12), (12), (10), (10), (10), (10), (10), (10), (10), (6), (6), (5), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Functional Studies (Func), Flow Cytometry (FACS)
(427), (208), (198), (127), (114), (93), (78), (47), (46), (24), (13), (10), (9), (4), (3), (2), (1), (1), (1), (1), (1), (1)
Pubmed 6 references available
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Product Details anti-CD59 Antibody

Target Details CD59 Application Details Handling References for anti-CD59 antibody (ABIN94204) Images
Specificity The antibody MEM-129 reacts with CD59 (Protectin), a 18-20 kDa glycosylphosphatidylinositol (GPI)-anchored glycoprotein expressed on all hematopoietic cells, it is widely present on cells in all tissues.
Purification Purified from ascites by thiophilic adsorption-affinity chromatography and size-exclusion chromatography.
Purity > 95 % (by SDS-PAGE)
Sterility 0.2 μm filtered
Immunogen Human peripheral blood lymphocytes
Clone MEM-129
Isotype IgM

Target Details CD59

Product Details anti-CD59 Antibody Application Details Handling References for anti-CD59 antibody (ABIN94204) Images back to top
Antigen
Alternative Name CD59 (CD59 Antibody Abstract)
Background CD59 (Protectin) is a small (18-20 kDa) GPI-anchored ubiquitously expressed inhibitor of the membrane attack complex (MAC). It is thus the key regulator that preserves the autologous cells from terminal effector mechanism of the complement cascade. CD59 associates with C5b-8 complex and thereby counteracts appropriate formation of cytolytic pore within the plasma membrane. CD59 is also an low-affinity ligand of human CD2 and causes T cell costimulation.
Pathways Complement System

Application Details

Product Details anti-CD59 Antibody Target Details CD59 Handling References for anti-CD59 antibody (ABIN94204) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-CD59 Antibody Target Details CD59 Application Details References for anti-CD59 antibody (ABIN94204) Images back to top
Concentration 1 mg/mL
Buffer Azide free phosphate buffered saline (PBS), approx. pH 7.4.
Preservative Azide free
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.

References for anti-CD59 antibody (ABIN94204)

Product Details anti-CD59 Antibody Target Details CD59 Application Details Handling Images back to top
Background publications

Baalasubramanian, Harris, Donev, Mizuno, Omidvar, Song, Morgan: "CD59a is the primary regulator of membrane attack complex assembly in the mouse." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 173, Issue 6, pp. 3684-92, 2004

Menu, Tsai, Bothwell, Sims, Bierer: "CD59 costimulation of T cell activation. CD58 dependence and requirement for glycosylation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 153, Issue 6, pp. 2444-56, 1994

Rooney, Davies, Griffiths, Williams, Davies, Meri, Lachmann, Morgan: "The complement-inhibiting protein, protectin (CD59 antigen), is present and functionally active on glomerular epithelial cells." in: Clinical and experimental immunology, Vol. 83, Issue 2, pp. 251-6, 1991

Meri, Morgan, Davies, Daniels, Olavesen, Waldmann, Lachmann: "Human protectin (CD59), an 18,000-20,000 MW complement lysis restricting factor, inhibits C5b-8 catalysed insertion of C9 into lipid bilayers." in: Immunology, Vol. 71, Issue 1, pp. 1-9, 1990

Product cited in:

Stulnig, Berger, Sigmund, Stockinger, Horejsí, Waldhäusl: "Signal transduction via glycosyl phosphatidylinositol-anchored proteins in T cells is inhibited by lowering cellular cholesterol." in: The Journal of biological chemistry, Vol. 272, Issue 31, pp. 19242-7, 1997

Olweus, Lund-Johansen, Terstappen: "CD64/Fc gamma RI is a granulo-monocytic lineage marker on CD34+ hematopoietic progenitor cells." in: Blood, Vol. 85, Issue 9, pp. 2402-13, 1995