anti-Human AKT antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended AKT Antibody (supplied by: Log in to see )

Antigen
V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) Antibodies
  • AKT
  • CWS6
  • PKB
  • PKB-ALPHA
  • PRKBA
  • RAC
  • RAC-ALPHA
  • Akt
  • Rac
  • PKB/Akt
  • PKBalpha
  • AKT/PKB
  • AKT1
  • Akt/PKB
  • Akt1
  • CG4006
  • D-Akt
  • DAKT1
  • DAKT1/PKB
  • DAkt
  • DAkt1
  • DPKB
  • DRAC-PK
  • DRAC-PK66
  • DRAC-PK85
  • Dakt
  • Dakt1
  • Dmel\\CG4006
  • Dpkb
  • PKB/AKT
  • PKB/dAKT
  • RacPK
  • akt
  • akt1
  • dAKT
  • dAKT/dPKB
  • dAKT1
  • dAkt
  • dAkt/PKB
  • dAkt1
  • dPKB
  • dakt
  • dakt1
  • l(3)04226
  • l(3)89Bq
  • p-Akt
  • pAkt
  • ACT-5
  • akt-1
  • pkb
  • v-akt
  • v-akt1
  • xAct
  • ATAKT1
  • F18A8.2
  • F18A8_2
  • K+ transporter 1
  • POTASSIUM TRANSPORTER
  • AKT serine/threonine kinase 1
  • thymoma viral proto-oncogene 1
  • CG4006 gene product from transcript CG4006-RE
  • actin beta
  • v-akt murine thymoma viral oncogene homolog 1 S homeolog
  • v-akt murine thymoma viral oncogene homolog 1
  • K+ transporter 1
  • Serine/threonine-protein kinase akt-1
  • AKT1
  • Akt1
  • ACTB
  • akt1.S
  • akt1
  • KT1
  • akt-1
Epitope
AA 445-480
216
112
108
58
57
48
37
32
30
26
25
21
19
18
18
18
14
13
12
12
12
12
12
12
10
10
9
9
8
7
7
6
6
6
6
6
5
5
5
5
5
5
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
1305
745
602
69
55
54
52
44
31
29
6
6
6
5
4
3
3
3
3
1
1
1
1
Host
Rabbit
1031
357
11
4
2
2
Clonality
Polyclonal
Conjugate
This AKT antibody is un-conjugated
55
43
39
39
31
26
12
12
7
7
7
5
5
5
5
5
5
4
4
4
4
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
Application
Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
1209
622
601
283
204
195
134
110
61
38
32
25
12
11
10
4
4
3
3
2
2
1
1
1
1
1
1
1
Options
Supplier
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Available images

Catalog No. ABIN725195
$ 318.45
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
27.778215 ABIN2854922 ICC IF IHC (p) WB Rabbit IgG Center Log in to see Polyclonal 0
27.778215 ABIN2854921 ICC IF IHC (p) WB Rabbit IgG N-Term Log in to see Polyclonal 0
20.138702 ABIN615269 EIA IHC (p) WB Rabbit Log in to see Polyclonal 0
19 ABIN1496555 FACS IF IHC IHC (p) WB Mouse IgG1 Log in to see 3E3 0
19 ABIN1496558 FACS IF IHC IHC (p) WB Mouse IgG1 Log in to see 4C11 0
19 ABIN1496557 FACS IF IHC IHC (p) WB Mouse IgG1 Log in to see 4E10 0
19 ABIN1496554 FACS IF IHC IHC (p) WB Mouse IgG1 Log in to see 4D6 0
19 ABIN1099896 ELISA IF IHC IHC (p) WB Mouse IgG1 pSer473 Log in to see 17F6-B11 0
19 ABIN1496556 FACS IF IHC IHC (p) WB Mouse IgG1 Log in to see 3B4 0
19 ABIN1496559 FACS IF IHC IHC (p) WB Mouse IgG1 Log in to see 1G5 0
19 ABIN559829 IF IHC (p) IP ELISA WB Mouse IgG2a kappa AA 1-480, full length Log in to see 2E11 0
17.138702 ABIN615022 EIA IF IHC (p) IP WB Rabbit C-Term Log in to see Polyclonal 0
17.138702 ABIN725180 IF (p) IHC (p) WB Mouse IgG AA 445-480 Log in to see Polyclonal 0
16 ABIN559832 IHC (p) ELISA WB Mouse IgG2a kappa AA 1-480, full length Log in to see 2H1 0
16 ABIN559833 IHC (p) ELISA WB Mouse IgG2a kappa AA 1-480, full length Log in to see 4B9 0
14.138702 ABIN950329 EIA FACS IF IHC (p) WB Rabbit Ig Fraction C-Term Log in to see Polyclonal 4
14.138702 ABIN950327 EIA IHC (p) WB Rabbit Ig Fraction AA 289-318 Log in to see Polyclonal 4
14.138702 ABIN214085 IHC IHC (p) WB Rabbit IgG AA 121-170 Log in to see Polyclonal 0
14.138702 ABIN213755 ELISA ICC IF IHC IHC (p) WB Rabbit pSer473 Log in to see Polyclonal 0
14.138702 ABIN5557411 IF (cc) IHC (p) WB Rabbit IgG pSer473 Log in to see 12A1 0

General

Antigen V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) Antibodies
Epitope AA 445-480
(216), (112), (108), (58), (57), (48), (37), (32), (30), (26), (25), (21), (19), (18), (18), (18), (14), (13), (12), (12), (12), (12), (12), (12), (10), (10), (9), (9), (8), (7), (7), (6), (6), (6), (6), (6), (5), (5), (5), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(1305), (745), (602), (69), (55), (54), (52), (44), (31), (29), (6), (6), (6), (5), (4), (3), (3), (3), (3), (1), (1), (1), (1)
Host Rabbit
(1031), (357), (11), (4), (2), (2)
Clonality
Polyclonal
Conjugate This AKT antibody is un-conjugated
(55), (43), (39), (39), (31), (26), (12), (12), (7), (7), (7), (5), (5), (5), (5), (5), (5), (4), (4), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1)
Application Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(1209), (622), (601), (283), (204), (195), (134), (110), (61), (38), (32), (25), (12), (11), (10), (4), (4), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 4 references available
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Product Details anti-AKT Antibody

Target Details AKT Application Details Handling References for anti-AKT antibody (ABIN725195) Images
Purification Purified by Protein A.
Immunogen KLH conjugated synthetic peptide derived from human AKT1
Isotype IgG
Plasmids, Primers & others

Target Details AKT

Product Details anti-AKT Antibody Application Details Handling References for anti-AKT antibody (ABIN725195) Images back to top
Antigen
Alternative Name AKT1 (AKT1 Antibody Abstract)
Background

AKT1-specific substrates have been recently identified, including palladin (PALLD), which phosphorylation modulates cytoskeletal organization and cell motility, prohibitin (PHB), playing an important role in cell metabolism and proliferation, and CDKN1A, for which phosphorylation at 'Thr-145' induces its release from CDK2 and cytoplasmic relocalization. These recent findings indicate that the AKT1 isoform has a more specific role in cell motility and proliferation. Phosphorylates CLK2 thereby controlling cell survival to ionizing radiation.

Subcellular location: Cytoplasm, Nucleus

Synonyms: AKT, PKB, RAC, CWS6, PRKBA, PKB-ALPHA, RAC-ALPHA, RAC-alpha serine/threonine-protein kinase, Protein kinase B, Protein kinase B alpha, PKB alpha, Proto-oncogene c-Akt, RAC-PK-alpha, AKT1, RAC-gamma serine/threonine-protein kinase, Protein kinase Akt-3, Protein kinase B gamma, PKB gamma, RAC-PK-gamma, STK-2, PKBG

Gene ID 207
UniProt P31749
Pathways PI3K-Akt Signaling, RTK Signaling, TCR Signaling, AMPK Signaling, Interferon-gamma Pathway, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Response to Water Deprivation, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, Thromboxane A2 Receptor Signaling, Signaling of Hepatocyte Growth Factor Receptor, Positive Regulation of fat Cell Differentiation, VEGFR1 Specific Signals, VEGF Signaling

Application Details

Product Details anti-AKT Antibody Target Details AKT Handling References for anti-AKT antibody (ABIN725195) Images back to top
Application Notes WB 1:100-1000
IHC-P 1:100-500
IF(IHC-P) 1:50-200
Restrictions For Research Use only

Handling

Product Details anti-AKT Antibody Target Details AKT Application Details References for anti-AKT antibody (ABIN725195) Images back to top
Format Liquid
Concentration 1 μg/μL
Buffer Aqueous buffered solution containing 1 % BSA, 50 % glycerol and 0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store at -20°C
Expiry Date 12 months

References for anti-AKT antibody (ABIN725195)

Product Details anti-AKT Antibody Target Details AKT Application Details Handling Images back to top
Product cited in:

Zhang, Zhao, Zhang, Hao, Yu, Min, Li, Ma, Chen, Yi, Tang, Meng, Liu, Wang, Shen, Zhang: "Decrease in male mouse fertility by hydrogen sulfide and/or ammonia can Be inheritable." in: Chemosphere, Vol. 194, pp. 147-157, 2018 Method employed by authors: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Sample species: Mouse (Murine)). Further details: Sample: testicular tissue

Zhao, Zhang, Liu, Zhang, Hao, Li, Chen, Shen, Tang, Min, Meng, Wang, Yi, Zhang: "Hydrogen Sulfide and/or Ammonia Reduces Spermatozoa Motility through AMPK/AKT Related Pathways." in: Scientific reports, Vol. 6, pp. 37884, 2016 Method employed by authors: Western Blotting (WB), Western Blotting (WB) (Sample species: Pig (Porcine)).

Li, Liu, Wang, Sun, Ding, Sun, Han, Wang: "Follistatin could promote the proliferation of duck primary myoblasts by activating PI3K/Akt/mTOR signalling." in: Bioscience reports, Vol. 34, Issue 5, 2014 Method employed by authors: Western Blotting (WB)

Xu, Huang, Xie, Lin, Guo, Zhuang, Lin, Zhou, Li, Huang, Wei, Man, Zhang: "The expression of cytoglobin as a prognostic factor in gliomas: a retrospective analysis of 88 patients." in: BMC cancer, Vol. 13, pp. 247, 2013 Method employed by authors: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Sample species: Human). Further details: Sample: glioma tissue

Images

Product Details anti-AKT Antibody Target Details AKT Application Details Handling References for anti-AKT antibody (ABIN725195) back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (AA 445-480) antibody (ABIN725195) Formalin-fixed and paraffin embedded: mouse transplant lymphoma labeled with Anti-PKB...
Western Blotting (WB) image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (AA 445-480) antibody (ABIN725195) Mouse placenta lysate with Anti-AKT1/3 Polyclonal Antibody Unconjugated at 1:5000 fo...
Western Blotting (WB) image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (AA 445-480) antibody (ABIN725195) Lane 1: Mouse Placenta lysates;Lane 2: Mouse Lung lysates probed with AKT1/3 Polyclon...
'Independent Validation' Badge
Lot Number 131113
Method validated Flow Cytometry
Positive Control MDA-MB-468 cells - lower expression
Notes A strong specific signal is observed in both of the positive control cells stained with anti-AKT1 plus secondary antibody compared with isotype, secondary only and unstained cells.
Primary Antibody
  • Antigen: V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1)
  • Catalog number: ABIN725195
  • Supplier: Log in to see
  • Supplier catalog number: Log in to see
  • Lot number: 131113
  • Dilution: 1 µg in 100 µL 1X PBS containing 0.5% BSA
Isotype Control Antibody
  • Antibody: Rabbit IgG
  • Catalog number: Log in to see
  • Supplier: Log in to see
  • Lot number: 16
  • Dilution: 1 µg in 100 µL 1X PBS containing 0.5% BSA
Secondary Antibody
  • Antibody: Goat anti-rabbit IgG-FITC
  • Supplier: Log in to see
  • Catalog number: Log in to see
  • Lot number: 115629
  • Dilution: 1:200 in 1X PBS containing 0.5% BSA
Controls
  • Positive control: MCF-7 cells
  • Low positive control: MDA-MB-468 cells
  • Isotype control: Both cell lines treated with rabbit IgG instead of the primary antibody to confirm that primary antibody binding is specific.
  • Secondary only control: Both cell lines treated with Goat anti-rabbit IgG-FITC to confirm no background signal produced from secondary antibody alone
Protocol
  • Control cells were cultured in DMEM + 10% FBS. - Control cells were washed once with phosphate-buffered saline (PBS) and harvested with a non-enzymatic cell dissociation solution (Cellstripper, Mediatech, Inc).
  • Detached cells were washed and resuspended in 0.5 mL 1X PBS containing 0.5% BSA.
  • An equivalent amount of pre-warmed 4% paraformaldehyde was added and the cells were incubated for 10 min at 37 °C.
  • Cells were washed in 1X PBS containing 0.5% BSA and resuspended in 1 mL of ice-cold 90% methanol for 30 min on ice.
  • Cells were spun out of the methanol and washed twice by resuspending in 1X PBS containing 0.5% BSA.
  • Cells were resuspended in 100 µL 1X PBS containing 0.5% BSA: - unstained cells - secondary antibody alone - isotype control antibody + secondary antibody - primary antibody + secondary antibody
  • Cells were incubated for 30 min on ice.
  • Labeled cells were washed twice in PBS containing 0.5% BSA.
  • Cells were resuspended with 1X PBS containing 0.5% BSA + 10% goat serum and incubated for 15 min at room temperature.
  • Goat anti-rabbit IgG-FITC secondary antibody (Jackson Immunoresearch) was added at a 1:200 dilution. The cells were incubated for 30 min in the dark on ice.
  • Labeled cells were washed twice in PBS containing 0.5% BSA.
  • Cells were analyzed on a FACSAria III (BD Biosciences) using a blue laser (488 nm excitation / 525 nm emission).
Experimental Notes Nothing to note.
Validation Images
Flow Cytometry validation image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (AA 445-480) antibody (ABIN725195) Figure 1: Positive control (high expression) MCF7 cells. The red histogram is unstain...
Flow Cytometry validation image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (AA 445-480) antibody (ABIN725195) Figure 2: Positive control (lower expression) MDA-MB-468 cells. The red histogram is ...