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|Antigen||Ubiquitin Interaction Motif Containing 1 (UIMC1) Antibodies|
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
|8 references available|
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|Specificity||Specificity of antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.|
|Purification||Immunogen affinity purified|
|Immunogen||This antibody was developed against Recombinant Protein corresponding to amino acids:LLRKAIAESLNSCRPSDASATRSRPLATGPSSQSHQEKTTDSGLTEGIWQLVPPSLFKGSHISQGNEAEEREEPWDHTEKTEEEPVSGSSG|
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|Alternative Name||RAP80 (UIMC1 Antibody Abstract)|
|Background||Gene Symbol: UIMC1|
|Pathways||DNA Damage Repair, Nuclear Hormone Receptor Binding, Positive Regulation of Response to DNA Damage Stimulus|
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|Application Notes||Western Blot 1:100 - 1:250, Immunohistochemistry 1:10-1:500, Immunocytochemistry/Immunofluorescence 1 - 4 μg/mL, Immunohistochemistry-Paraffin 1:50-1:200For IHC-Paraffin, HIER pH 6 retrieval is recommended. IF fixation/permeabilization: PFA/Triton X-100.|
The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.
|Restrictions||For Research Use only|
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PBS ( pH 7.2) and 40 % Glycerol
Buffer contains: 0.02 % Sodium Azide
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage||4 °C,-20 °C|
|Storage Comment||Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.|
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|Product cited in:||
Baranes-Bachar, Levy-Barda, Oehler, Reid, Soria-Bretones, Voss, Chung, Park, Liu, Yoon, Li, Dellaire, Misteli, Huertas, Rothenberg, Ramadan, Ziv, Shiloh: "The Ubiquitin E3/E4 Ligase UBE4A Adjusts Protein Ubiquitylation and Accumulation at Sites of DNA Damage, Facilitating Double-Strand Break Repair." in: Molecular cell, Vol. 69, Issue 5, pp. 866-878.e7, 2019 (Sample species: Human).
Bodo, Campagne, Thin, Higginson, Vargas, Hua, Fuller, Ackerstaff, Russell, Zhang, Klingler, Cho, Kaag, Mazaheri, Rimner, Manova-Todorova, Epel, Zatcky, Cleary, Rao, Yamada, Zelefsky, Halpern et al.: "Single-dose radiotherapy disables tumor cell homologous recombination via ischemia/reperfusion injury. ..." in: The Journal of clinical investigation, Vol. 129, Issue 2, pp. 786-801, 2019 Method employed by authors: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Sample species: Human).
Watanabe, Iimori, Chan, Hara, Kitao, Maehara: "MDC1 methylation mediated by lysine methyltransferases EHMT1 and EHMT2 regulates active ATM accumulation flanking DNA damage sites." in: Scientific reports, Vol. 8, Issue 1, pp. 10888, 2018 Method employed by authors: Immunofluorescence (fixed cells) (IF/ICC) (Sample species: Human).
Yasuhara, Kato, Hagiwara, Shiotani, Yamauchi, Nakada, Shibata, Miyagawa: "Human Rad52 Promotes XPG-Mediated R-loop Processing to Initiate Transcription-Associated Homologous Recombination Repair." in: Cell, Vol. 175, Issue 2, pp. 558-570.e11, 2018 (Sample species: Human).
Ha, Ma, Lin, Tang, Lian, Zhao, Li, Zhen, Pei, Han, Malumbres, Jin, Chen, Zhao, Zhu, Zhang: "The anaphase promoting complex impacts repair choice by protecting ubiquitin signalling at DNA damage sites." in: Nature communications, Vol. 8, pp. 15751, 2017 (Sample species: Human).
Renaud, Barascu, Rosselli: "Impaired TIP60-mediated H4K16 acetylation accounts for the aberrant chromatin accumulation of 53BP1 and RAP80 in Fanconi anemia pathway-deficient cells." in: Nucleic acids research, Vol. 44, Issue 2, pp. 648-56, 2016 Method employed by authors: Immunohistochemistry (IHC)
Lin, Ratner, Whicker, Lee, Sartorelli: "Triapine disrupts CtIP-mediated homologous recombination repair and sensitizes ovarian cancer cells to PARP and topoisomerase inhibitors." in: Molecular cancer research : MCR, Vol. 12, Issue 3, pp. 381-93, 2014
Kato, Nakajima, Ui, Muto-Terao, Ogiwara, Nakada: "Fine-tuning of DNA damage-dependent ubiquitination by OTUB2 supports the DNA repair pathway choice." in: Molecular cell, Vol. 53, Issue 4, pp. 617-30, 2014 (Sample species: Human). Further details: Immunocytochemistry,Immunofluorescence
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