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|Antigen||Calcitonin-Related Polypeptide alpha (CALCA) Antibodies|
|Reactivity||Human, Mammalian, Rat (Rattus) Alternatives|
|Conjugate||This CGRP antibody is un-conjugated Alternatives|
Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))
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Product Details anti-CGRP AntibodyTarget Details CGRP Application Details Handling Images
|Specificity||This antibody is specific for Calcitonin Gene Related Peptide. Also recognizes rat beta-CGRP and human alpha-CGRP (10-fold less sensitivity compared to rat alpha-CGRP). Suggested Positive Controls: Dorsal root ganglia, pancreas and gut.|
Species reactivity (expected):most Mammals.
Species reactivity (tested):Human and Rat.
|Characteristics||Synonyms: Calcitonin gene-related peptide I, Alpha-type CGRP, CALC1, CGRP-I|
|Purification||Protein G Chromatography.|
|Plasmids, Primers & others|
Target Details CGRPProduct Details anti-CGRP Antibody Application Details Handling Images back to top
|Alternative Name||CGRP / CALCA (CALCA Antibody Abstract)|
|Background||Calcitonin Gene Related Peptide [CGRP] is a 37 amino acid peptide and is the most potent endogenous vasodilator currently known (Brain et al.,1985). CGRP is primarily produced in nervous tissue, however, its receptors are expressed throughout the body. CGRP is also strongly implicated in the vasodilatory effect of endogenous cannabinoid anandamide in the brain. This effect was found to be antagonized by capsazepine. CGRP is also currently a major target of research in regards to factors effecting the onset of migraine headaches (Edvinsson L., 2003). CGRP antibodies have been used successfully in Immunoneutralization (Wong et al., 1993)Synonyms: Alpha-type CGRP, CALC1, CGRP-I, Calcitonin gene-related peptide I|
|Pathways||Hormone Activity, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Regulation of G-Protein Coupled Receptor Protein Signaling, Skeletal Muscle Fiber Development, Feeding Behaviour|
Application DetailsProduct Details anti-CGRP Antibody Target Details CGRP Handling Images back to top
Immunocytochemistry: 1/50-1/100. Immunofluorescence and Immunohistochemistry: This antibody is an excellent tool forImmunohistochemistry and for multiple labeling immunofluorescence of peripheral tissuelike stomach, intestine, pancreas, sensory ganglia, and all the regions of the body that areinnervated by primary afferent neurons as well as central nervous tissue. Suggested Positive Controls: Dorsal root ganglia, pancreas and gut. Not recommended for Western blotting.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-CGRP Antibody Target Details CGRP Application Details Images back to top
|Buffer||10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50 % Glycerol|
Store the antibody undiluted (in aliquots) at -20 °C. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
|Expiry Date||12 months|