anti-Human Islet Amyloid Polypeptide antibody for Immunohistochemistry (Frozen Sections)

Recommended Islet Amyloid Polypeptide Antibody (supplied by: Log in to see )

Antigen
Islet Amyloid Polypeptide (IAPP) Antibodies
  • DAP
  • IAP
  • LOC100230121
  • Amylin
  • islet amyloid polypeptide
  • IAPP
  • Iapp
Reactivity
Human
118
28
18
10
6
5
4
3
1
1
1
1
1
Host
Mouse
122
14
Clonality
Monoclonal
Conjugate
Un-conjugated
12
7
5
1
Application
Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC)
70
63
54
25
16
14
12
10
5
4
4
2
2
2
2
1
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Catalog No. ABIN153560
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN118954 IHC (fro) IF IHC (p) Mouse IgG1 Log in to see R10-99 7
1 ABIN218499 IF IHC IHC (fro) IHC (p) Mouse IgG1 Log in to see R10-99 0
1 ABIN3201496 ICC IHC (fro) IHC (p) ELISA WB Mouse IgG Log in to see 0
1 ABIN2477416 IHC (fro) IF IHC (p) Mouse IgG1 Log in to see R10-99 3
1 ABIN221316 IHC IHC (fro) IHC (p) Rabbit IgG Log in to see Polyclonal 0
1 ABIN1839628 IHC IHC (fro) IHC (p) Rabbit Log in to see Polyclonal 0
1 ABIN319879 IHC (fro) IHC (p) Mouse IgG1 Log in to see R10-99 1
1 ABIN115737 IHC (fro) IHC (p) Rabbit Log in to see Polyclonal 2
1 ABIN1105348 EIA IHC (fro) WB Rabbit N-Term Log in to see Polyclonal 0
1 ABIN1724505 IHC (fro) IHC (p) Mouse IgG1 Log in to see R10-99 0
1 ABIN285658 IHC (fro) IHC (p) Rabbit IgG1 Log in to see Polyclonal 0
1 ABIN5580450 IF IHC (fro) IHC (p) Mouse IgG1 Log in to see R10-99 0
1 ABIN5691119 IHC (fro) IHC (p) Rabbit Ig Mix Log in to see Polyclonal 0
1 ABIN2767742 IHC (fro) IHC (p) IHC Mouse IgG1 Log in to see R10-99 0
1 ABIN643064 IHC (fro) IHC (p) Mouse IgG1 Log in to see 0
1 ABIN643065 IHC (fro) IHC (p) Mouse IgG1 Log in to see 0

General

Antigen Islet Amyloid Polypeptide (IAPP) Antibodies
Reactivity Human
(118), (28), (18), (10), (6), (5), (4), (3), (1), (1), (1), (1), (1)
Host Mouse
(122), (14)
Clonality (Clone)
Monoclonal   ( )
Conjugate Un-conjugated
(12), (7), (5), (1)
Application Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC)
(70), (63), (54), (25), (16), (14), (12), (10), (5), (4), (4), (2), (2), (2), (2), (1)
Pubmed 6 references available
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Product details

Target Details Application Details Handling References Images
Specificity The is of high affinity, and stains islet amyloid peptide in intra-cellular insulin granules, intra-cellular amyloid and extra-cellular islet amyloid.
Purification Tissue culture supernatant
Immunogen Synthetic peptide: CATQRLANFLV, corresponding to internal sequence amino acids 40-50 of Human Amylin coupled to tuberculin.
Clone R10-99
Isotype IgG1

Target Details

Product details Application Details Handling References Images back to top
Antigen
Alternative Name Amylin (IAPP Antibody Abstract)
Background Gene Symbol: IAPP
Gene ID 3375
UniProt P10997
Pathways Hormone Activity, Feeding Behaviour

Application Details

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Application Notes Immunohistochemistry 1:10-1:500, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunohistochemistry-Paraffin 1:100, Immunohistochemistry-Frozen 1:100IHC-P: Use at a dilution of 1/100. Antigen retrieval is not essential but may optimise staining.IHC-Fr: Use at a dilution of 1/100. This product does not require protein digestion pre-treatment of paraffin sections. This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product details Target Details Application Details References Images back to top
Format Liquid
Buffer Tissue culture supernatant
Buffer contains: 0.09 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

References

Product details Target Details Application Details Handling Images back to top
Product cited in:

Gurlo, Ryazantsev, Huang, Yeh, Reber, Hines, OBrien, Glabe, Butler: "Evidence for proteotoxicity in beta cells in type 2 diabetes: toxic islet amyloid polypeptide oligomers form intracellularly in the secretory pathway." in: The American journal of pathology, Vol. 176, Issue 2, pp. 861-9, 2010

Zhao, Guan, Lee, Sui, He, Siu, Tse, Tong, Lai, Chan: "Up-regulated pancreatic tissue microRNA-375 associates with human type 2 diabetes through beta-cell deficit and islet amyloid deposition." in: Pancreas, Vol. 39, Issue 6, pp. 843-6, 2010

Nakamura, Okabayashi, Ageyama, Koie, Sankai, Ono, Fujimoto, Terao: "Transthyretin amyloidosis and two other aging-related amyloidoses in an aged vervet monkey." in: Veterinary pathology, Vol. 45, Issue 1, pp. 67-72, 2008

Zhao, Sui, Guan, He, Gu, Wong, Baum, Lai, Tong, Chan: "Amyloid oligomers in diabetic and nondiabetic human pancreas." in: Translational research : the journal of laboratory and clinical medicine, Vol. 153, Issue 1, pp. 24-32, 2008

Bhattacharya, Latha, Kumresan, Singh: "Cloning and expression of human islet amyloid polypeptide in cultured cells." in: Biochemical and biophysical research communications, Vol. 356, Issue 3, pp. 622-8, 2007

Gong, Liu, Zeng, Peng, Chen, Zhou, Li: "Amylin deposition in the kidney of patients with diabetic nephropathy." in: Kidney international, Vol. 72, Issue 2, pp. 213-8, 2007

Images

Product details Target Details Application Details Handling References back to top
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Islet Amyloid Polypeptide (IAPP) antibody (ABIN153560) Immunohistochemistry-Paraffin: Amylin Antibody (R10/99) [ABIN153560] - Human pancreas...
Immunofluorescence (Cultured Cells) (IF (cc)) image for anti-Islet Amyloid Polypeptide (IAPP) antibody (ABIN153560) Immunocytochemistry/Immunofluorescence: Amylin Antibody (R10/99) - Staining of paraf...