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|Antigen||Islet Amyloid Polypeptide (IAPP) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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|Specificity||This antibody detects endogenous levels of Amylin protein. (region surrounding His28)|
Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.
|Purification||The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen|
|Purity||> 95 % pure by SDS-PAGE|
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|Alternative Name||Amylin / IAPP (IAPP Antibody Abstract)|
|Background||Adrenomedullin (AM), alpha- and beta-calcitonin gene-related peptide(CGRP), calcitonin (CT), and amylin are homologous polypeptides with overlapping biological actions such as vasodilatation and inhibition of bone resorption. Amylin (Islet/insulinoma amyloid polypeptide or IAPP) is a 37-amino acid monomeric polypeptide isolated from pancreatic amyloid. This protein, with a calculated molecular mass of 4 kDa, is a major component of amyloid-rich pancreatic extracts of three type 2 diabetic patients. IAPP has cysteine residues in positions 2 and 7, a feature found in all known calcitonin gene-related peptides. IAPP shows 46 % amino acid sequence homology with CGRP II. Since IAPP has been demonstrated immunochemically in normal beta cells of several mammals, it probably has an important role in respect to pancreatic islet function.Synonyms: Diabetes-associated peptide, Insulinoma amyloid peptide, Islet amyloid polypeptide|
|Molecular Weight||approx. 10 kDa|
|Pathways||Hormone Activity, Feeding Behaviour|
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ELISA: 1: 10000approx. 1: 20000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
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|Buffer||Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||DO NOT FREEZE!|
|Storage Comment||Store the antibody undiluted at 2-8 °C.|
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