Hsd3b3 Protein (AA 1-373) (His tag)
custom-made
HSD3B3
Origin: Mouse
Host: HEK-293 Cells
Recombinant
> 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
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- Target
- Hsd3b3 (HSD3B3) (Hydroxy-delta-5-Steroid Dehydrogenase, 3 beta and Steroid delta-Isomerase 3 (HSD3B3))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-373
- Origin
- Mouse
- Source
- HEK-293 Cells
- Purification tag / Conjugate
- This Hsd3b3 protein is labelled with His tag.
- Purpose
- Predefined custom protein recombinant Hsd3b3 Protein expressed in mammalian cells.
- Sequence
- MPGWSCLVTG AGGFLGQRII QLLVQEKDLE EIRVLDKVFK PETREQFFNL GTSIKVTVLE GDILDTQYLR RACQGISVVI HTAAIIDVTG VIPRQTILDV NLKGTQNLLE ACIQASVPAF IFSSSVDVAG PNSYKDIVLN GHEDEHREST WSDPYPYSKK MAEKAVLAAN GSMLKNGGTL QTCALRPMCI YGERSQFLSN TIIKALKNKF ILRGGGKFST ANPVYVGNVA WAHILAARGL RNPKKSPNIQ GEFYYISDDT PHQSYDDLNY TLSKEWGFCL NSRWYLPVPI LYWLAFLLET VSFLLSPIYR YIPPFNRHLV TLTASTFTFS YKKAQRDLGY EPLVSWEEAK QKTSEWIGTL VEQHRETLDT KSQ Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.
- Specificity
- If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.
- Characteristics
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Key Benefits:
- Predefined custom protein - from design to production - by highly experienced protein experts.
- Protein expressed in mammalian cells and purified in one-step affinity chromatography
- The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
If you are not interested in a full length protein, please contact us for individual protein fragments.
The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified. - Purity
- > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
- Grade
- custom-made
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- Application Notes
- We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- 12 months
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- Target
- Hsd3b3 (HSD3B3) (Hydroxy-delta-5-Steroid Dehydrogenase, 3 beta and Steroid delta-Isomerase 3 (HSD3B3))
- Alternative Name
- Hsd3b3
- Background
- 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 3 (3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type III) (3-beta-HSD III) [Includes: 3-beta-hydroxy-Delta(5)-steroid dehydrogenase (EC 1.1.1.145) (3-beta-hydroxy-5-ene steroid dehydrogenase) (Progesterone reductase), Steroid Delta-isomerase (EC 5.3.3.1) (Delta-5-3-ketosteroid isomerase)],FUNCTION: 3-beta-HSD is a bifunctional enzyme, that catalyzes the oxidative conversion of Delta(5)-ene-3-beta-hydroxy steroid, and the oxidative conversion of ketosteroids. The 3-beta-HSD enzymatic system plays a crucial role in the biosynthesis of all classes of hormonal steroids.
- Molecular Weight
- 42.0 kDa
- UniProt
- P26150
- AlphaFold
- P26150
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