HSV1/2 IgM ELISA Kit
This Human HSV1/2 IgM ELISA Kit is a Colorimetric ELISA Kit designed to quantify Human HSV1/2 IgM.
Catalog No. ABIN7666555
Quick Overview for HSV1/2 IgM ELISA Kit (ABIN7666555)
Target
See all HSV1/2 IgM products
HSV1/2 IgM
(Anti-Herpes Simplex Virus Type 1/2 IgM (HSV1/2 IgM))
Reactivity
Human
Detection Method
Colorimetric
Method Type
Indirect ELISA
Application
ELISA
Sample Type
Plasma, Serum
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Purpose
- anti-HSV 1+2 IgM assay is a solid-phase immunoanalytical test. T
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Brand
- ELISA-VIDITEST
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Analytical Method
- Semi-Quantitative
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Components
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- ELISA break-away strips in the handling frame coated with the specific antigen STRIPS Ag 1 x 12 pcs
- 1.3 mL Negative control human serum, r.t.u. 1) NC 1 vial
- 1.3 mL Positive control human serum, r.t.u. PC 1 vial
- 2.0 mL Calibrator (human serum), r.t.u. CAL 1 vial
- 13 mL Anti-human IgM animal antibodies labelled with horseradish peroxidase (anti-IgM Px conjugate) r.t.u. CONJ 1 vial
- 55 mL Wash buffer, 10x concentrated WASH 10x 1 vial
- 60 mL Dilution buffer, r.t.u. DIL 1 vial
- 13 mL Chromogenic substrate TMB, r.t.u. (TMB/H2O 2) TMB 1 vial
- 13 mL Stop solution, r.t.u. (0.4 M sulfuric acid) STOP 1 vial
- 2 mL RF-sorbent (anti-human IgG) 25 x concentrated RF SORB 25x 1 vial
- Instruction manual
- Quality Control Certificate
- 1) r.t.u., ready to use / mailto:info@vidia.cz
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Material not included
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- Distilled or deionised water for dilution of buffer and standard concentrates
- appropriate and calibrated equipment for pipetting
- temperature controlled incubator
- spectrophotometer or platereader with the appropriate filters
- empty microtiter plate for pre-incubation
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Plate
- Pre-coated
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Protocol
- anti-HSV 1+2 IgM assay is a solid-phase immunoanalytical test. The polystyrene strips are coated with a native antigens containing immunodominant epitopes of HSV 1 and 2. Anti-HSV antibodies from samples bind to the immobilized antigens. The other serum antibodies do not bind and are washed away those that formed complexes with the antigens are later on recognised by animal anti-human IgM antibodies labelled with horseradish peroxidase. The presence of the labelled antibodies is revealed by an enzymatic reaction with a chromogenic substrate. Negative samples do not react and the mild change in colour, if present, may be attributed to the reaction background.
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Restrictions
- For Research Use only
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Storage
- 4 °C
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- HSV1/2 IgM (Anti-Herpes Simplex Virus Type 1/2 IgM (HSV1/2 IgM))
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Alternative Name
- HSV1+2 IgM
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Target Type
- Antibody
Target See all HSV1/2 IgM products
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