anti-Chicken Integrin alpha 4 antibody for Radioimmunoassay

Recommended Integrin alpha 4 Antibody

Antigen
Integrin alpha 4 (ITGA4) Antibodies
  • CD49D
  • IA4
  • LOC100219853
  • integrin subunit alpha 4
  • integrin alpha 4
  • integrin subunit alpha 4 S homeolog
  • ITGA4
  • Itga4
  • itga4.S
Epitope
C-Term
32
16
11
6
6
5
4
4
3
3
3
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Chicken, Hamster, Human, Mouse (Murine), Rat (Rattus)
339
239
106
20
13
13
12
10
9
8
8
4
3
2
2
2
2
2
2
2
1
1
1
Host
Rabbit
176
153
144
1
1
Clonality
Polyclonal
Conjugate
Un-conjugated
58
57
32
12
10
7
7
5
3
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
Application
Immunoprecipitation (IP), Immunohistochemistry (IHC), Radioimmunoassay (RIA), ELISA, Western Blotting (WB)
324
121
112
98
63
40
34
31
23
13
7
6
5
4
4
4
3
2
2
1
1
Options
Catalog No. ABIN219734
$ 947.83
Plus shipping costs $45.00

General

Antigen Integrin alpha 4 (ITGA4) Antibodies
Epitope C-Term
(32), (16), (11), (6), (6), (5), (4), (4), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Chicken, Hamster, Human, Mouse (Murine), Rat (Rattus)
(339), (239), (106), (20), (13), (13), (12), (10), (9), (8), (8), (4), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Host Rabbit
(176), (153), (144), (1), (1)
Clonality
Polyclonal
Conjugate Un-conjugated
(58), (57), (32), (12), (10), (7), (7), (5), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Application Immunoprecipitation (IP), Immunohistochemistry (IHC), Radioimmunoassay (RIA), ELISA, Western Blotting (WB)
(324), (121), (112), (98), (63), (40), (34), (31), (23), (13), (7), (6), (5), (4), (4), (4), (3), (2), (2), (1), (1)
Supplier

Product details

Target Details Application Details Handling Images
Specificity Recognizes human Integrin alpha 4. Specificity determined by immunoprecipitation from a detergent extract of 35S methionine-labeled lymphoid cells. No cross-reactivity to alpha 1, alpha 2, alpha 3, alpha 5, alpha 6 or alpha V. Recognizes an epitope on the cytoplasmic domain of the protein. Epitope is intracellular. Species cross-reactivity: rat, chicken, hamster and mouse.
Purification Antiserum
Immunogen Synthetic peptide derived from the C-terminal sequence of the human alpha 4 integrin subunit. Cellular Localization: Cytoplasmic, Intracellular.

Type of Immunogen: Synthetic peptide
Isotype IgG

Target Details

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Antigen
Alternative Name ITGA4 / VLA-4 / CD49d (ITGA4 Antibody Abstract)
Background Name/Gene ID: ITGA4
Family: Integrin

Synonyms: ITGA4, 269C wild type, CD49D, IA4, Integrin alpha-4, Integrin alpha 4, Integrin alpha-4 subunit, Integrin alpha-IV, VLA-4, VLA-4 subunit alpha, CD49d antigen
Gene ID 3676
UniProt P13612
Pathways Integrin Complex

Application Details

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Application Notes Approved: ELISA (1:1000), IHC (1:250), IP, RIA (1:500), WB (1:1000)

Usage: Suitable for use in ELISA, Radioimmunoassay, Western Blot, Immunohistochemistry and Immunoprecipitation. ELISA/RIA: 1:500. Western Blot (non-reduced): 1:500. Blocking should be with 1-3 % BSA. Recognizes a 140kD band under non-reducing conditions. Immunohistochemistry: 1:250. Immunoprecipitation: 5 μL to precipitate alpha4 from 5x1 x106.
Comment

Target Species of Antibody: Human

Restrictions For Research Use only

Handling

Product details Target Details Application Details Images back to top
Format Liquid
Concentration Lot specific
Buffer Supplied as a liquid, 0.05 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice avoid freeze thaw cycles. Store undiluted.
Storage 4 °C,-20 °C
Storage Comment Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Store undiluted.