anti-Human CrkL antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended CrkL Antibody (supplied by: Log in to see )

Antigen
V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian)-Like (CRKL) Antibodies
  • CRKL
  • wu:fa55g05
  • wu:fj60e01
  • zgc:63469
  • 1110025F07Rik
  • AA589403
  • AI325100
  • Crkol
  • CRK like proto-oncogene, adaptor protein
  • v-crk avian sarcoma virus CT10 oncogene homolog-like
  • CRKL
  • crkl
  • Crkl
Reactivity
Human, Mouse (Murine), Rat (Rattus)
186
101
72
4
2
2
1
Host
Rabbit
140
43
24
1
Clonality
Polyclonal
Conjugate
This CrkL antibody is un-conjugated
10
9
9
8
6
5
4
4
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
166
89
43
25
23
22
19
7
7
5
4
3
2
2
2
Supplier
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Catalog No. ABIN498177
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
10 ABIN2888596 ELISA IF IHC IHC (p) WB Rabbit IgG AA 173-222 Log in to see Polyclonal 0
10 ABIN703420 IF (p) IHC (p) WB Rabbit IgG AA 230-270, pTyr251 Log in to see Polyclonal 0
10 ABIN5575996 ELISA IF IHC (p) WB Rabbit Log in to see Polyclonal 0
10 ABIN5575997 IHC (p) WB Rabbit IgG Log in to see Polyclonal 0
8.494791 ABIN498833 IHC (p) Rabbit pTyr207 Log in to see Polyclonal 0
8.494791 ABIN498921 IHC (p) Rabbit Log in to see Polyclonal 0
7 ABIN962645 ELISA IHC IHC (p) WB Goat AA 292-303 Log in to see Polyclonal 0
7 ABIN703646 IF (p) IHC (p) WB Rabbit IgG AA 235-285 Log in to see Polyclonal 0
6.974512 ABIN268650 IHC (p) IHC ELISA WB Goat C-Term Log in to see Polyclonal 1
5.474512 ABIN4300532 IHC IHC (p) WB Rabbit IgG Log in to see Polyclonal 0
4 ABIN1733317 IHC (p) ELISA WB Goat C-Term Log in to see Polyclonal 0
4 ABIN5575993 ELISA IHC (p) WB Rabbit pTyr207 Log in to see Polyclonal 0
4 ABIN5575995 ELISA IHC (p) WB Rabbit Log in to see Polyclonal 0
1 ABIN703430 IHC (p) WB HRP Rabbit IgG AA 230-270, pTyr251 Log in to see Polyclonal 0
1 ABIN703655 IHC (p) WB HRP Rabbit IgG AA 235-285 Log in to see Polyclonal 0
1 ABIN2883672 ELISA IF IHC IHC (p) WB Rabbit IgG pTyr207 Log in to see Polyclonal 0
1 ABIN2891928 IF IHC IHC (p) WB Rabbit Pro201 Log in to see Polyclonal 0
1 ABIN2893828 IHC IHC (p) IP WB Rabbit pTyr207 Log in to see Polyclonal 0
1 ABIN703422 IHC (p) WB Biotin Rabbit IgG AA 230-270, pTyr251 Log in to see Polyclonal 0
1 ABIN703648 IHC (p) WB Biotin Rabbit IgG AA 235-285 Log in to see Polyclonal 0

General

Antigen V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian)-Like (CRKL) Antibodies
Reactivity Human, Mouse (Murine), Rat (Rattus)
(186), (101), (72), (4), (2), (2), (1)
Host Rabbit
(140), (43), (24), (1)
Clonality
Polyclonal
Conjugate This CrkL antibody is un-conjugated
(10), (9), (9), (8), (6), (5), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(166), (89), (43), (25), (23), (22), (19), (7), (7), (5), (4), (3), (2), (2), (2)
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Product Details anti-CrkL Antibody

Target Details CrkL Application Details Handling Images
Specificity This antibody detects endogenous levels of CrkL protein.
Cross-Reactivity (Details) Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.
Purification Affinity Chromatography using epitope-specific immunogen
Plasmids, Primers & others

Target Details CrkL

Product Details anti-CrkL Antibody Application Details Handling Images back to top
Antigen
Alternative Name CRKL (CRKL Antibody Abstract)
Background SH2 and SH3 (Src homology) domains were originally identified as critical functional domains within non-receptor proteins with tyrosine kinase activity. A subset of these proteins appears to exist predominately of SH2/SH3 domains in the absence of detectable catalytic domains. One of the first members of the family to be identified, Crk, is a transformation-specific protein that induces elevation of cellular phosphotyrosine levels, but lacks tyrosine kinase activity itself. A second protein, Nck, consists solely of three SH3 domains and one SH2 domain, while GRB2 contains an SH2 domain flanked on both sides by SH3 domains. A member of this protein class, Crk-L, is encoded by a gene located on chromosome 22, band 11, centromeric of the chronic myelogenous leukemia breakpoint region. Crk-L encodes a 303 amino acid protein with one SH2 and two SH3 domains, and a predicted molecular weight of 36 kDa.Synonyms: Crk-like protein, v-crk avian sarcoma virus CT10 oncogene homolog-like
Molecular Weight approx. 39 kDa
Gene ID 1399
NCBI Accession NP_005198
UniProt P46109
Pathways Interferon-gamma Pathway, Neurotrophin Signaling Pathway, Signaling of Hepatocyte Growth Factor Receptor

Application Details

Product Details anti-CrkL Antibody Target Details CrkL Handling Images back to top
Application Notes Western blot: 1: 500 - 1: 1000. Immunohistochemistry on paraffin sections: 1: 50 - 1: 200. Immunoflourescence: 1: 50 - 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-CrkL Antibody Target Details CrkL Application Details Images back to top
Concentration 1.0 mg/mL
Buffer Phosphate buffered saline (PBS), pH ~7.2 with 0.05 % Sodium Azide as preservative
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8° for one month or (in aliquots) at -20 °C for longer.

Images

Product Details anti-CrkL Antibody Target Details CrkL Application Details Handling back to top
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian)-Like (CRKL) antibody (ABIN498177) Immunohistochemistry analyzes of CrkL antibody in paraffin-embedded human lung carcin...
Western Blotting (WB) image for anti-V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian)-Like (CRKL) antibody (ABIN498177) Western blot analysis of CrkL antibody in extracts from COS7 cells.