anti-Human p300 antibody for Immunohistochemistry (Paraffin-embedded Sections)
Recommended p300 Antibody (supplied by: Log in to see )
- Antigen
-
E1A Binding Protein P300 (EP300) Antibodies
show synonyms for this antigen- KAT3B
- RSTS2
- p300
- A430090G16
- A730011L11
- p300 HAT
- E1A binding protein p300
- histone acetyltransferase p300
- EP300
- LOC446056
- ep300
- Ep300
- Epitope
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AA 1000-1050
Alternatives - Reactivity
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Human
Alternatives - Host
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Rabbit
Alternatives - Clonality
- Conjugate
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This p300 antibody is un-conjugated
Alternatives - Application
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Chromatin Immunoprecipitation (ChIP), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
Alternatives - Supplier
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- Supplier Product No.
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Available images
| Relevance Score | ABIN | Application | Conjugate | Host | Isotype | Epitope | Supplier | Clonality | References | Details |
|---|---|---|---|---|---|---|---|---|---|---|
| 13.042537 | ABIN498122 | IHC (p) WB | Rabbit | Log in to see | Polyclonal | 0 | ||||
| 13.042537 | ABIN800613 | IF (p) IHC (p) | Rabbit | IgG | pSer1834 | Log in to see | Polyclonal | 0 | ||
| 13.042537 | ABIN4328394 | ICC IF IHC IHC (p) | Rabbit | IgG | Log in to see | Polyclonal | 0 | |||
| 13.042537 | ABIN4328393 | ICC IF IHC IHC (p) | Rabbit | IgG | Log in to see | Polyclonal | 0 | |||
| 1 | ABIN3042372 | IHC (p) WB | Rabbit | IgG | AA 2065-2414 | Log in to see | Polyclonal | 0 | ||
| 1 | ABIN358187 | EIA IHC (p) WB | Rabbit | Ig Fraction | pSer89 | Log in to see | Polyclonal | 5 | ||
| 1 | ABIN389618 | DB IHC (p) | Rabbit | Ig Fraction | pSer89 | Log in to see | Polyclonal | 2 | ||
| 1 | ABIN389698 | DB IHC (p) | Rabbit | Ig Fraction | pSer1834 | Log in to see | Polyclonal | 1 | ||
| 1 | ABIN782395 | EIA IF IHC (p) WB | Mouse | IgG1 | AA 731-831 | Log in to see | 1D2 | 0 | ||
| 1 | ABIN2879000 | ELISA IHC IHC (p) WB | Rabbit | IgG | AA 1-50 | Log in to see | Polyclonal | 0 | ||
| 1 | ABIN790522 | ELISA IF IHC IHC (p) WB | Mouse | IgG1 | AA 731-831 | Log in to see | 1B1 | 0 | ||
| 1 | ABIN790523 | ELISA IF IHC IHC (p) WB | Mouse | IgG1 | AA 731-831 | Log in to see | 1D2 | 0 | ||
| 1 | ABIN560748 | IF IHC (p) ELISA WB | Mouse | IgG1 | AA 731-830, partial | Log in to see | 1B1 | 1 | ||
| 1 | ABIN515310 | IF IHC (p) ELISA WB | Mouse | IgG1 | AA 731-830, partial | Log in to see | 1D2 | 0 | ||
| 1 | ABIN3188089 | ELISA IHC (p) WB | Rabbit | IgG | Log in to see | Polyclonal | 0 | |||
| 1 | ABIN272047 | IHC (p) WB | Rabbit | Log in to see | Polyclonal | 0 | ||||
| 1 | ABIN782394 | EIA IF IHC (p) WB | Mouse | IgG1 | AA 731-831 | Log in to see | 1B1 | 0 | ||
| 1 | ABIN3030784 | IHC (p) WB | Rabbit | IgG | AA 2065-2414 | Log in to see | Polyclonal | 0 | ||
| 1 | ABIN2884158 | ELISA IHC IHC (p) | Rabbit | IgG | pSer89 | Log in to see | Polyclonal | 0 | ||
| 1 | ABIN2891869 | IHC IHC (p) WB | Rabbit | Val6 | Log in to see | Polyclonal | 0 |
General |
|
|---|---|
| Antigen | E1A Binding Protein P300 (EP300) Antibodies |
| Epitope | AA 1000-1050 Alternatives |
| Reactivity | Human Alternatives |
| Host | Rabbit Alternatives |
| Clonality | |
| Conjugate | This p300 antibody is un-conjugated Alternatives |
| Application |
Chromatin Immunoprecipitation (ChIP), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
Alternatives
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4 references available |
| Supplier | Log in to see |
Product Details anti-p300 AntibodyTarget Details p300 Application Details Handling References for anti-p300 antibody (ABIN152981) Images |
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| Purification | Immunogen affinity purified |
| Immunogen | The immunogen this antibody was made to, maps to a region between residues 1000 and 1050 of human E1A-associated protein p300 using the numbering given in entry NP_001420.2 (GeneID 2033). |
| Plasmids, Primers & others | |
Target Details p300Product Details anti-p300 Antibody Application Details Handling References for anti-p300 antibody (ABIN152981) Images back to top |
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| Antigen | |
| Alternative Name | KAT3B/p300 (EP300 Antibody Abstract) |
| Background | Gene Symbol: EP300 |
| Gene ID | 2033 |
| UniProt | Q09472 |
| Pathways | p53 Signaling, Notch Signaling, Interferon-gamma Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Lipid Metabolism by PPARalpha, Regulation of Muscle Cell Differentiation, Regulation of Cell Size |
Application DetailsProduct Details anti-p300 Antibody Target Details p300 Handling References for anti-p300 antibody (ABIN152981) Images back to top |
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| Application Notes | Western Blot 1:1000-1:10000, Chromatin Immunoprecipitation 1:10-1:500, Immunohistochemistry 1:500 to 1:2000, Immunocytochemistry/Immunofluorescence 1:250 - 1:1000, Immunoprecipitation 1-4 μg/mg of lysate, Immunohistochemistry-Paraffin 1:500 to 1:2000Western blot and immunoprecipitation. ChIP reported successful by customer review. Immunohistochemistry 1:500 to 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. |
| Comment |
The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo. |
| Restrictions | For Research Use only |
HandlingProduct Details anti-p300 Antibody Target Details p300 Application Details References for anti-p300 antibody (ABIN152981) Images back to top |
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| Format | Liquid |
| Concentration | 1.0 mg/mL |
| Buffer |
Tris-Citrate/Phosphate ( pH 7.0 - 8.0) Buffer contains: 0.09 % Sodium Azide |
| Preservative | Sodium azide |
| Precaution of Use | This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. |
| Handling Advice | Do not freeze. |
| Storage | 4 °C |
| Storage Comment | Store at 4°C. Do not freeze. |
References for anti-p300 antibody (ABIN152981)Product Details anti-p300 Antibody Target Details p300 Application Details Handling Images back to top |
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| Product cited in: |
Lee, Huang, Chang, Huang, Hsieh, Chen, Chou, Chiang, Lai, Shiau: "Genome-wide analysis of HIF-2α chromatin binding sites under normoxia in human bronchial epithelial cells (BEAS-2B) suggests its diverse functions." in: Scientific reports, Vol. 6, pp. 29311, 2016 Method employed by authors: Immunohistochemistry (IHC) Luo, Chen, Chen, Alkam, Wang, Semenza: "PRDX2 and PRDX4 are negative regulators of hypoxia-inducible factors under conditions of prolonged hypoxia." in: Oncotarget, Vol. 7, Issue 6, pp. 6379-97, 2016 Method employed by authors: Western Blotting (WB) Luo, Hu, Chang, Zhong, Knabel, OMeally, Cole, Pandey, Semenza: "Pyruvate kinase M2 is a PHD3-stimulated coactivator for hypoxia-inducible factor 1." in: Cell, Vol. 145, Issue 5, pp. 732-44, 2011 Julien, Carriere, Moreau, Roux: "mTORC1-activated S6K1 phosphorylates Rictor on threonine 1135 and regulates mTORC2 signaling." in: Molecular and cellular biology, Vol. 30, Issue 4, pp. 908-21, 2010 |
ImagesProduct Details anti-p300 Antibody Target Details p300 Application Details Handling References for anti-p300 antibody (ABIN152981) back to top |
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| Supplier Images |
anti-E1A Binding Protein P300 (EP300) (AA 1000-1050) antibody
Western Blot: KAT3B/p300 Antibody [ABIN152981] - Nuclear (NE) or cytosolic (S100) ext...
Immunohistochemistry-Paraffin: KAT3B/p300 Antibody [ABIN152981] - Human lung carcinoma.
Western Blot: KAT3B/p300 Antibody - Detection of Human p300 by Western Blot. Samples...
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