anti-Human p300 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended p300 Antibody (supplied by: Log in to see )

Antigen
E1A Binding Protein P300 (EP300) Antibodies
  • KAT3B
  • RSTS2
  • p300
  • A430090G16
  • A730011L11
  • p300 HAT
  • E1A binding protein p300
  • histone acetyltransferase p300
  • EP300
  • LOC446056
  • ep300
  • Ep300
Epitope
AA 2065-2414
58
43
39
19
18
11
6
5
4
4
4
3
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
269
125
111
38
37
3
3
3
3
3
2
Host
Rabbit
182
98
Clonality
Polyclonal
Conjugate
This p300 antibody is un-conjugated
11
11
11
8
8
6
4
4
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
192
113
97
74
56
47
47
38
28
11
7
5
3
2
2
1
1
1
1
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Catalog No. ABIN3042372
$ 280.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
18.583267 ABIN3188089 ELISA IHC (p) WB Rabbit IgG Log in to see Polyclonal 0
17.5 ABIN560748 IF IHC (p) ELISA WB Mouse IgG1 AA 731-830, partial Log in to see 1B1 1
16 ABIN515310 IF IHC (p) ELISA WB Mouse IgG1 AA 731-830, partial Log in to see 1D2 0
13 ABIN782395 EIA IF IHC (p) WB Mouse IgG1 AA 731-831 Log in to see 1D2 0
13 ABIN3030784 IHC (p) WB Rabbit IgG AA 2065-2414 Log in to see Polyclonal 0
11.5 ABIN389698 DB IHC (p) Rabbit Ig Fraction pSer1834 Log in to see Polyclonal 1
11.127563 ABIN498122 IHC (p) WB Rabbit Log in to see Polyclonal 0
8.127563 ABIN800613 IF (p) IHC (p) Rabbit IgG pSer1834 Log in to see Polyclonal 0
7 ABIN358187 EIA IHC (p) WB Rabbit Ig Fraction pSer89 Log in to see Polyclonal 5
7 ABIN389618 DB IHC (p) Rabbit Ig Fraction pSer89 Log in to see Polyclonal 2
7 ABIN790522 ELISA IF IHC IHC (p) WB Mouse IgG1 AA 731-831 Log in to see 1B1 0
7 ABIN790523 ELISA IF IHC IHC (p) WB Mouse IgG1 AA 731-831 Log in to see 1D2 0
7 ABIN782394 EIA IF IHC (p) WB Mouse IgG1 AA 731-831 Log in to see 1B1 0
7 ABIN5577324 IHC (p) WB Rabbit Log in to see Polyclonal 0
7 ABIN5577326 IF IHC (p) Rabbit IgG Log in to see Polyclonal 0
4 ABIN2879000 ELISA IHC IHC (p) WB Rabbit IgG AA 1-50 Log in to see Polyclonal 0
4 ABIN5956441 ELISA IHC (p) WB Rabbit IgG Ser89 Log in to see Polyclonal 0
4 ABIN272047 IHC (p) WB Rabbit Log in to see Polyclonal 0
4 ABIN1734067 IHC (p) ELISA WB Rabbit IgG Log in to see Polyclonal 0
4 ABIN1723866 IF IHC (p) ELISA WB Mouse IgG1 AA 731-831 Log in to see 1D2 0

General

Antigen E1A Binding Protein P300 (EP300) Antibodies
Epitope AA 2065-2414
(58), (43), (39), (19), (18), (11), (6), (5), (4), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(269), (125), (111), (38), (37), (3), (3), (3), (3), (3), (2)
Host Rabbit
(182), (98)
Clonality
Polyclonal
Conjugate This p300 antibody is un-conjugated
(11), (11), (11), (8), (8), (6), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(192), (113), (97), (74), (56), (47), (47), (38), (28), (11), (7), (5), (3), (2), (2), (1), (1), (1), (1)
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Product Details anti-p300 Antibody

Target Details p300 Application Details Handling Images
Purpose Rabbit IgG polyclonal antibody for Histone acetyltransferase p300(EP300) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Cross-Reactivity (Details) No cross reactivity with other proteins.
Characteristics Rabbit IgG polyclonal antibody for Histone acetyltransferase p300(EP300) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: E1A binding protein p300
Protein Name: Histone acetyltransferase p300
Purification Immunogen affinity purified.
Immunogen E.coli-derived human KAT3B recombinant protein (Position: L2065-H2414). Human KAT3B shares 94% amino acid (aa) sequence identity with mouse KAT3B.
Isotype IgG
Plasmids, Primers & others

Target Details p300

Product Details anti-p300 Antibody Application Details Handling Images back to top
Antigen
Alternative Name EP300 (EP300 Antibody Abstract)
Background E1A binding protein p300 also known as EP300 or p300 is a protein that in humans is encoded by the EP300 gene. The EP300 gene is located on the long (q) arm of the human chromosome 22 at position 13.2. This protein regulates the activity of many genes in tissues throughout the body. It plays an essential role in regulating cell growth and division, prompting cells to mature and assume specialized functions (differentiate), and preventing the growth of cancerous tumors. The EP300 protein appears to be critical for normal development before and after birth. It carries out its function by activating transcription. In addition, the protein functions as histone acetyltransferase that regulates transcription via chromatin remodeling, and is important in the processes of cell proliferation and differentiation. EP300 also mediates cAMP-gene regulation by binding specifically to phosphorylated CREB protein.

Synonyms: CREBBP/EP300 inhibitory protein 1 antibody|Cyclic AMP responsive enhancer binding protein antibody|E1A associated protein p300 antibody|E1A binding protein p300 antibody|E1A-associated protein p300 antibody|EC 2.3.1.48 antibody|EP300 antibody|EP300: E1A binding protein p300 antibody|EP300_HUMAN antibody|Histone acetyltransferase p300 antibody|KAT3B antibody|p300 HAT antibody|RB and P300 binding protein EID 1 antibody|Retinoblastoma protein associated protein antibody|RSTS2 antibody
Gene ID 2033
UniProt Q09472
Pathways p53 Signaling, Notch Signaling, Interferon-gamma Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Lipid Metabolism by PPARalpha, Regulation of Muscle Cell Differentiation, Regulation of Cell Size

Application Details

Product Details anti-p300 Antibody Target Details p300 Handling Images back to top
Application Notes WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, The detection limit for KAT3B is approximately 0.25 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
Comment

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions For Research Use only

Handling

Product Details anti-p300 Antibody Target Details p300 Application Details Images back to top
Format Lyophilized
Reconstitution Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
Concentration 500 μg/mL
Buffer Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Images

Product Details anti-p300 Antibody Target Details p300 Application Details Handling back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-E1A Binding Protein P300 (EP300) (AA 2065-2414) antibody (ABIN3042372) Anti- KAT3B/p300 Picoband antibody, IHC(P) IHC(P): Mouse Intestine Tissue
Western Blotting (WB) image for anti-E1A Binding Protein P300 (EP300) (AA 2065-2414) antibody (ABIN3042372) Anti- KAT3B/p300 Picoband antibody, Western blotting All lanes: All lanes: Anti KAT3B...
Western Blotting (WB) image for anti-E1A Binding Protein P300 (EP300) (AA 2065-2414) antibody (ABIN3042372) anti-E1A Binding Protein P300 (EP300) (AA 2065-2414) antibody (Image 3)
Immunohistochemistry (IHC) image for anti-E1A Binding Protein P300 (EP300) (AA 2065-2414) antibody (ABIN3042372) Anti- KAT3B/p300 Picoband antibody, IHC(P) IHC(P): Rat Intestine Tissue
Immunohistochemistry (IHC) image for anti-E1A Binding Protein P300 (EP300) (AA 2065-2414) antibody (ABIN3042372) Anti- KAT3B/p300 Picoband antibody, IHC(P) IHC(P): Human Oesophagus Squama Cancer Tissue
 image for anti-E1A Binding Protein P300 (EP300) (AA 2065-2414) antibody (ABIN3042372) Western blot analysis of KAT3B/p300 using anti- KAT3B/p300 antibody . Electrophoresi...