Rat IgG2a Isotype Control
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- Target See all IgG2a products
- IgG2a
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Host
- Rat
- Application
- Flow Cytometry (FACS), Isotype Control (IsoC)
- Brand
- BD Pharmingen™
- Characteristics
- The R35-95 hybridoma was generated by hybridization of Y3 myeloma cells with spleen cells from LOU rats immunized with mouse immunoglobulins. The R35-95 hybridoma produces rat IgG2a, κ immunoglobulin that has no measurable reactivity with mouse immunoglobulins. The R35-95 immunoglobulin was selected as an isotype control following screening for low background binding on a variety of mouse and human tissues. Profile of peripheral blood lymphocytes analyzed on a FACScan (BDIS, San Jose, CA).
- Purification
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- Sterility
- 0.2 μm filtered
- Endotoxin Level
- Endotoxin level is ≤0.01 EU/μg (≤0.001 ng/μg) of protein as determined by the LAL assay.
- Immunogen
- Mouse Pooled Immunoglobulin
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Clone
- R35-95
- Isotype
- IgG2a
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1.0 mg/mL
- Buffer
- No azide/low endotoxin: Aqueous buffered solution containing no preservative
- Preservative
- Azide free
- Storage
- 4 °C
- Storage Comment
- Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.
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- Target
- IgG2a
- Abstract
- IgG2a Products
- Synonyms
- gamma-2a immunoglobulin heavy chain Isotype Control, IgG-2a Isotype Control
- Target Type
- Antibody
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