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Human IgM isotype control (FITC)

IsoC, IF IgM FITC F(ab')2 fragment
Catalog No. ABIN930170
  • Target See all IgM products
    IgM
    Fragment
    F(ab')2 fragment
    Host
    • 54
    • 36
    • 34
    • 5
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    Human
    Conjugate
    FITC
    Application
    Isotype Control (IsoC), Immunofluorescence (IF)
    Characteristics
    Human IgM protein (Fab mu) (FITC) conjugate
    Source: Human serum
    Alternative Names: Immunoglobulin M protein (Human) (Fab mu) (FITC)
    Purification
    Human IgM protein (Fab mu) (FITC) was purified by delipidation, salt fractionation, ion exchange chromatography and dialysis.
    Isotype
    IgM
  • Application Notes
    Optimal working dilutions should be determined experimentally by the investigator.
    Comment

    Excitation/Emission wavelength: 494 nm/514 nm

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Concentration
    Lot specific
    Buffer
    Lyophilized from 0.02 M K2 O4, pH 7.2, with 0.12 NaCl, 10 mg/mL BSA and 0.01 % NaN3. Immunoglobulin and protease free.
    Preservative
    Sodium azide
    Precaution of Use
    WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
    Handling Advice
    Product is photosensitive and should be protected from light.
    Avoid repeated freeze/thaw cycles.
    Storage
    4 °C/-20 °C
    Storage Comment
    Store at 4 °C until reconstitution. Following reconstitution aliquot and freeze at -20 °C for long term storage.
  • Target
    IgM
    Abstract
    IgM Products
    Synonyms
    IgM constant region Isotype Control, IGM Isotype Control
    Target Type
    Antibody
    Background
    Immunoglobulin M, or IgM for short, is a basic antibody that is produced by B cells. It is the primary antibody against A and B antigens on red blood cells. IgM is by far the physically largest antibody in the human circulatory system. It is the first antibody to appear in response to initial exposure to antigen. The fragment antigen-binding (Fab fragment) is a region on an antibody that binds to antigens. It is composed of one constant and one variable domain of each of the heavy and the light chain. These domains shape the paratope - the antigen-binding site - at the amino terminal end of the monomer. The two variable domains bind the epitope on their specific antigens.
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