anti-Cow (Bovine) CD25 antibody for Immunohistochemistry

Recommended CD25 Antibody (supplied by: Log in to see )

Antigen
Interleukin 2 Receptor, alpha (IL2RA) Antibodies
  • CD25
  • IDDM10
  • IL2R
  • TCGFR
  • IL2RAC
  • IL-2RA
  • Il2r
  • Ly-43
  • interleukin 2 receptor subunit alpha
  • interleukin-2 receptor subunit alpha-like
  • interleukin 2 receptor, alpha chain
  • IL2RA
  • Il2ra
  • LOC100222957
Epitope
AA 22-243
23
21
19
18
16
15
15
13
13
13
7
6
6
6
6
4
4
3
3
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
Reactivity
Cow (Bovine)
643
477
172
22
19
15
9
7
6
6
5
4
4
4
3
1
Host
Rabbit
633
398
168
7
5
3
3
2
Clonality
Polyclonal
Conjugate
This CD25 antibody is un-conjugated
133
126
102
82
29
28
27
23
22
17
17
15
13
11
11
11
11
11
10
10
9
8
8
7
7
7
7
6
6
6
5
4
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunohistochemistry (IHC), Western Blotting (WB)
990
224
177
156
129
125
82
78
55
29
20
20
17
17
16
13
8
8
7
6
5
4
2
2
1
1
1
1
1
Options
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Catalog No. ABIN1862763
$ 400.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN5625392 FACS Func IHC Mouse IgG2a Log in to see CACT108A 0
1 ABIN5625390 FACS Func IHC Mouse IgG1 Log in to see Bu-CACT116A 0
1 ABIN5625393 FACS Func IHC Mouse IgG1 Log in to see CACT116A 0
1 ABIN601651 FACS IHC IHC (fro) IP Mouse IgG1 Log in to see 0
1 ABIN576883 FACS IHC IHC (fro) IP PE Mouse IgG1 Log in to see IL-A111 0
1 ABIN5625396 FACS Func IHC Mouse IgG1 Log in to see GB112A 0
1 ABIN5625399 FACS Func IHC Mouse IgG3 Log in to see LCTB2A 0
1 ABIN2288556 FACS IP IHC Mouse IgG1 Log in to see 10B1132 0
1 ABIN206834 FACS IHC IHC (fro) IP FITC Mouse IgG1 Log in to see 0
1 ABIN2935451 IF/ICC IHC IP WB Rabbit AA 22-243 Log in to see Polyclonal 0
1 ABIN6024434 IF/ICC IHC IP WB Mouse Log in to see 0

General

Antigen Interleukin 2 Receptor, alpha (IL2RA) Antibodies
Epitope AA 22-243
(23), (21), (19), (18), (16), (15), (15), (13), (13), (13), (7), (6), (6), (6), (6), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine)
(643), (477), (172), (22), (19), (15), (9), (7), (6), (6), (5), (4), (4), (4), (3), (1)
Host Rabbit
(633), (398), (168), (7), (5), (3), (3), (2)
Clonality
Polyclonal
Conjugate This CD25 antibody is un-conjugated
(133), (126), (102), (82), (29), (28), (27), (23), (22), (17), (17), (15), (13), (11), (11), (11), (11), (11), (10), (10), (9), (8), (8), (7), (7), (7), (7), (6), (6), (6), (5), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunohistochemistry (IHC), Western Blotting (WB)
(990), (224), (177), (156), (129), (125), (82), (78), (55), (29), (20), (20), (17), (17), (16), (13), (8), (8), (7), (6), (5), (4), (2), (2), (1), (1), (1), (1), (1)
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Product Details anti-CD25 Antibody

Target Details CD25 Application Details Handling Images
Specificity The antibody is a rabbit polyclonal antibody raised against IL2Ra. It has been selected for its ability to recognize IL2Ra in immunohistochemical staining and western blotting.
Purification Antigen-specific affinity chromatography
Immunogen IL2Ra (Glu22-Gln243)
Isotype IgG
Plasmids, Primers & others

Target Details CD25

Product Details anti-CD25 Antibody Application Details Handling Images back to top
Antigen
Alternative Name Interleukin 2 Receptor Alpha (IL2RA Antibody Abstract)
Background Alternative Names: CD25, IL2R-a, IL2-RA, IDDM10, IL2R, TCGFR, p55, TAC antigen
Pathways JAK-STAT Signaling, Growth Factor Binding, Activated T Cell Proliferation

Application Details

Product Details anti-CD25 Antibody Target Details CD25 Handling Images back to top
Application Notes
  • Western blotting: 1:50-400 Immunocytochemistry in formalin fixed cells: 1:50-500 Immunohistochemistry in formalin fixed frozen section: 1:50-500 Immunohistochemistry in paraffin section: 1:10-100 Enzyme-linked Immunosorbent Assay: 1:100-1:5000 Optimal working dilutions must be determined by end user.
Comment

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions For Research Use only

Handling

Product Details anti-CD25 Antibody Target Details CD25 Application Details Images back to top
Format Liquid
Concentration 200 μg/mL
Buffer PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage 4 °C
Storage Comment Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.
Expiry Date 12 months

Images

Product Details anti-CD25 Antibody Target Details CD25 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-Interleukin 2 Receptor, alpha (IL2RA) (AA 22-243) antibody (ABIN1862763) anti-Interleukin 2 Receptor, alpha (IL2RA) (AA 22-243) antibody