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|Antigen||Interleukin 2 Receptor, alpha (IL2RA) Antibodies|
|Epitope||AA 22-213 Alternatives|
|Conjugate||This CD25 antibody is un-conjugated Alternatives|
Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS), Neutralization (Neut), Western Blotting (WB)
|4 references available|
|Supplier||Log in to see|
Product Details anti-CD25 AntibodyTarget Details CD25 Application Details Handling References for anti-CD25 antibody (ABIN4899652) Images
|Specificity||Detects human CD25/IL-2 R alpha in direct ELISAs and Western blots. In direct ELISAs, less than 15 % cross-reactivity with recombinant human (rh) IL-2 sR gamma is observed, less than 5 % cross-reactivity with rhIL-6 sR is observed, and less than 1 % cross-reactivity with rhIL-1 sRII and rhIL-2 sR beta is observed. In direct ELISAs, less than 5 % cross-reactivity with recombinant mouse IL-2 R alpha is observed.|
|Purification||Immunogen affinity purified|
|Sterility||0.2 μm filtered|
|Endotoxin Level||<0.10 EU per 1 μg of the antibody by the LAL method.|
|Immunogen||S. frugiperda insect ovarian cell line Sf 21-derived recombinant human CD25/IL-2 R alpha , Glu22-Cys213, Accession # P01589|
|Plasmids, Primers & others|
Target Details CD25Product Details anti-CD25 Antibody Application Details Handling References for anti-CD25 antibody (ABIN4899652) Images back to top
|Alternative Name||CD25/IL-2 R alpha (IL2RA Antibody Abstract)|
|Background||Alternative Names: CD25, CD25 antigen, IDDM10, IL-2 R alpha, IL-2 receptor subunit alpha, IL2R, IL2R alpha, IL-2R subunit alpha, IL-2Ra, IL2RA, IL-2-RA, IL2-RA, interleukin 2 receptor, alpha, interleukin-2 receptor subunit alpha, p55, TAC antigen, TCGFR|
|Pathways||JAK-STAT Signaling, Growth Factor Binding, Activated T Cell Proliferation|
Application DetailsProduct Details anti-CD25 Antibody Target Details CD25 Handling References for anti-CD25 antibody (ABIN4899652) Images back to top
Western Blot 0.1 μg/mL
Flow Cytometry 2.5 μg/10^6 cells
Neutralization 0.2-1 μg/mL
|Restrictions||For Research Use only|
HandlingProduct Details anti-CD25 Antibody Target Details CD25 Application Details References for anti-CD25 antibody (ABIN4899652) Images back to top
|Reconstitution||Reconstitute at 0.2 mg/mL in sterile PBS.|
|Buffer||Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.|
|Storage||4 °C,-20 °C,-80 °C|
|Storage Comment||Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt,-20°C to -70°C as supplied. 1 month,2°C to 8°C under sterile conditions after reconstitution. 6 months,-20°C to -70°C under sterile conditions after reconstitution.|
|Expiry Date||12 months|
References for anti-CD25 antibody (ABIN4899652)Product Details anti-CD25 Antibody Target Details CD25 Application Details Handling Images back to top
|Product cited in:||
Zheng, Jones, Shi, Wiseman, Marr, Berenger, Huston, Gill, Krensky, Kubes, Mody: "Late expression of granulysin by microbicidal CD4+ T cells requires PI3K- and STAT5-dependent expression of IL-2Rbeta that is defective in HIV-infected patients." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 180, Issue 11, pp. 7221-9, 2008 Method employed by authors: Western Blotting (WB), Western Blotting (WB) (Sample species: Human).
Binder, Vögtle, Michelfelder, Müller, Illerhaus, Sundararajan, Mertelsmann, Trepel: "Identification of their epitope reveals the structural basis for the mechanism of action of the immunosuppressive antibodies basiliximab and daclizumab." in: Cancer research, Vol. 67, Issue 8, pp. 3518-23, 2007 Method employed by authors: Flow Cytometry (FACS), Flow Cytometry (FACS) (Sample species: Human).
Li, Ma, Wang, Li: "Interleukin-2alpha receptor in membrane lipid rafts." in: Transplantation proceedings, Vol. 37, Issue 5, pp. 2395-7, 2005 Method employed by authors: Western Blotting (WB), Western Blotting (WB) (Sample species: Human).
Sandrin, Muriaux, Darlix, Cosset: "Intracellular trafficking of Gag and Env proteins and their interactions modulate pseudotyping of retroviruses." in: Journal of virology, Vol. 78, Issue 13, pp. 7153-64, 2004 Method employed by authors: Western Blotting (WB), Western Blotting (WB) (Sample species: Human).
ImagesProduct Details anti-CD25 Antibody Target Details CD25 Application Details Handling References for anti-CD25 antibody (ABIN4899652) back to top