anti-Human CD25 antibody for Cytometry by Time of Flight

Recommended CD25 Antibody (supplied by: Log in to see )

Antigen
Interleukin 2 Receptor, alpha (IL2RA) Antibodies
  • CD25
  • IDDM10
  • IL2R
  • TCGFR
  • IL2RAC
  • IL-2RA
  • Il2r
  • Ly-43
  • interleukin 2 receptor subunit alpha
  • interleukin-2 receptor subunit alpha-like
  • interleukin 2 receptor, alpha chain
  • IL2RA
  • Il2ra
  • LOC100222957
Epitope
AA 22-213
22
20
20
19
18
15
15
14
13
13
7
6
6
6
5
4
3
3
3
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
Reactivity
Human
593
480
166
22
19
15
9
8
6
6
5
4
4
4
3
1
Host
Goat
590
406
153
7
4
3
3
2
Clonality
Polyclonal
Conjugate
This CD25 antibody is un-conjugated
132
119
96
79
30
29
29
24
23
18
16
14
12
12
12
12
12
11
10
10
9
8
8
7
7
6
6
6
4
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS), Neutralization (Neut), Western Blotting (WB)
941
189
170
156
126
90
87
83
61
29
21
20
18
17
13
12
9
8
7
6
6
5
3
2
1
1
1
1
Supplier
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Catalog No. ABIN4899652
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN269014 CyTOF FACS Mouse IgG1 Log in to see MEM-181 1
1 ABIN4899653 CyTOF FACS WB Mouse IgG2a AA 22-213 Log in to see 24212 3
1 ABIN4291069 BP CyTOF FACS IF IHC Neut WB Rabbit IgG AA 116-122, Extracellular Domain Log in to see Basiliximab
1 ABIN4291070 BP CyTOF FACS IF IHC Neut WB Rabbit IgG AA 116-122, Extracellular Domain Log in to see Basiliximab
1 ABIN4291067 BP CyTOF FACS Neut Rabbit IgG Log in to see Daclizumab
1 ABIN4291068 BP CyTOF FACS Neut Rabbit IgG Log in to see Daclizumab
1 ABIN269086 CyTOF FACS IP Mouse IgM Log in to see MEM-140
1 ABIN4291048 CyTOF FACS IF Mouse IgG1 kappa Log in to see 143-13
1 ABIN4291047 CyTOF FACS IF Mouse IgG1 kappa Log in to see 143-13

General

Antigen Interleukin 2 Receptor, alpha (IL2RA) Antibodies
Epitope AA 22-213
(22), (20), (20), (19), (18), (15), (15), (14), (13), (13), (7), (6), (6), (6), (5), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity Human
(593), (480), (166), (22), (19), (15), (9), (8), (6), (6), (5), (4), (4), (4), (3), (1)
Host Goat
(590), (406), (153), (7), (4), (3), (3), (2)
Clonality
Polyclonal
Conjugate This CD25 antibody is un-conjugated
(132), (119), (96), (79), (30), (29), (29), (24), (23), (18), (16), (14), (12), (12), (12), (12), (12), (11), (10), (10), (9), (8), (8), (7), (7), (6), (6), (6), (4), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS), Neutralization (Neut), Western Blotting (WB)
(941), (189), (170), (156), (126), (90), (87), (83), (61), (29), (21), (20), (18), (17), (13), (12), (9), (8), (7), (6), (6), (5), (3), (2), (1), (1), (1), (1)
Pubmed 4 references available
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Product Details anti-CD25 Antibody

Target Details CD25 Application Details Handling References for anti-CD25 antibody (ABIN4899652) Images
Specificity Detects human CD25/IL-2 R alpha in direct ELISAs and Western blots. In direct ELISAs, less than 15 % cross-reactivity with recombinant human (rh) IL-2 sR gamma is observed, less than 5 % cross-reactivity with rhIL-6 sR is observed, and less than 1 % cross-reactivity with rhIL-1 sRII and rhIL-2 sR beta is observed. In direct ELISAs, less than 5 % cross-reactivity with recombinant mouse IL-2 R alpha is observed.
Purification Immunogen affinity purified
Sterility 0.2 μm filtered
Endotoxin Level <0.10 EU per 1 μg of the antibody by the LAL method.
Immunogen S. frugiperda insect ovarian cell line Sf 21-derived recombinant human CD25/IL-2 R alpha , Glu22-Cys213, Accession # P01589
Isotype IgG

Target Details CD25

Product Details anti-CD25 Antibody Application Details Handling References for anti-CD25 antibody (ABIN4899652) Images back to top
Antigen
Alternative Name CD25/IL-2 R alpha (IL2RA Antibody Abstract)
Background Alternative Names: CD25, CD25 antigen, IDDM10, IL-2 R alpha, IL-2 receptor subunit alpha, IL2R, IL2R alpha, IL-2R subunit alpha, IL-2Ra, IL2RA, IL-2-RA, IL2-RA, interleukin 2 receptor, alpha, interleukin-2 receptor subunit alpha, p55, TAC antigen, TCGFR
Gene ID 3559
UniProt P01589
Pathways JAK-STAT Signaling, Growth Factor Binding, Activated T Cell Proliferation

Application Details

Product Details anti-CD25 Antibody Target Details CD25 Handling References for anti-CD25 antibody (ABIN4899652) Images back to top
Application Notes Western Blot 0.1 μg/mL
Flow Cytometry 2.5 μg/10^6 cells
Neutralization 0.2-1 μg/mL
CyTOF-ready
Restrictions For Research Use only

Handling

Product Details anti-CD25 Antibody Target Details CD25 Application Details References for anti-CD25 antibody (ABIN4899652) Images back to top
Reconstitution Reconstitute at 0.2 mg/mL in sterile PBS.
Buffer Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Preservative Without preservative
Storage 4 °C,-20 °C,-80 °C
Storage Comment Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt,-20°C to -70°C as supplied. 1 month,2°C to 8°C under sterile conditions after reconstitution. 6 months,-20°C to -70°C under sterile conditions after reconstitution.
Expiry Date 12 months

References for anti-CD25 antibody (ABIN4899652)

Product Details anti-CD25 Antibody Target Details CD25 Application Details Handling Images back to top
Product cited in:

Zheng, Jones, Shi, Wiseman, Marr, Berenger, Huston, Gill, Krensky, Kubes, Mody: "Late expression of granulysin by microbicidal CD4+ T cells requires PI3K- and STAT5-dependent expression of IL-2Rbeta that is defective in HIV-infected patients." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 180, Issue 11, pp. 7221-9, 2008 Method employed by authors: Western Blotting (WB) (Sample species: Human).

Binder, Vögtle, Michelfelder, Müller, Illerhaus, Sundararajan, Mertelsmann, Trepel: "Identification of their epitope reveals the structural basis for the mechanism of action of the immunosuppressive antibodies basiliximab and daclizumab." in: Cancer research, Vol. 67, Issue 8, pp. 3518-23, 2007 Method employed by authors: Flow Cytometry (FACS) (Sample species: Human).

Li, Ma, Wang, Li: "Interleukin-2alpha receptor in membrane lipid rafts." in: Transplantation proceedings, Vol. 37, Issue 5, pp. 2395-7, 2005 Method employed by authors: Western Blotting (WB) (Sample species: Human).

Sandrin, Muriaux, Darlix, Cosset: "Intracellular trafficking of Gag and Env proteins and their interactions modulate pseudotyping of retroviruses." in: Journal of virology, Vol. 78, Issue 13, pp. 7153-64, 2004 Method employed by authors: Western Blotting (WB) (Sample species: Human).

Images

Product Details anti-CD25 Antibody Target Details CD25 Application Details Handling References for anti-CD25 antibody (ABIN4899652) back to top
Supplier Images
Neutralization (Neut) image for anti-Interleukin 2 Receptor, alpha (IL2RA) (AA 22-213) antibody (ABIN4899652) Cell Proliferation Induced by IL-2 and Neutralization by Human CD25/IL-2 R alpha Anti...