anti-Human CD25 antibody for Western Blotting

Recommended CD25 Antibody (supplied by: Log in to see )

Antigen
Interleukin 2 Receptor, alpha (IL2RA) Antibodies
  • CD25
  • IDDM10
  • IL2R
  • TCGFR
  • IL2RAC
  • IL-2RA
  • Il2r
  • Ly-43
  • interleukin 2 receptor subunit alpha
  • interleukin-2 receptor subunit alpha-like
  • interleukin 2 receptor, alpha chain
  • IL2RA
  • Il2ra
  • LOC100222957
Epitope
AA 34-139
23
21
18
18
16
16
15
15
13
13
8
7
7
6
5
4
4
3
3
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Monkey, Rat (Rattus)
655
480
167
22
19
15
8
6
6
6
5
4
4
4
3
1
Host
Mouse
627
400
174
12
6
4
3
2
Clonality
Monoclonal
Conjugate
This CD25 antibody is un-conjugated
130
123
101
82
29
29
28
24
22
18
17
15
13
11
11
11
11
11
10
10
9
9
9
9
8
8
8
6
6
6
5
4
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
993
223
182
154
132
127
81
79
55
30
20
20
18
17
17
13
8
8
7
6
5
4
2
2
1
1
1
1
1
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Catalog No. ABIN1724861
$ 430.10
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.0788064 ABIN2705770 WB Rabbit Center Log in to see Polyclonal 0
3.0788064 ABIN3044139 IHC (p) WB Rabbit IgG AA 39-57, N-Term Log in to see Polyclonal 0
3.0788064 ABIN4880789 ELISA ICC IHC WB Mouse IgG1 AA 34-139 Log in to see 1B5D12 0
3.0788064 ABIN1845343 ELISA IF/ICC IHC WB Mouse IgG1 AA 34-139 Log in to see 0
3.0788064 ABIN1531665 IF ELISA WB Rabbit IgG AA 223-272, pSer268 Log in to see Polyclonal 2
3.0788064 ABIN1532689 IF ELISA WB Rabbit IgG AA 223-272 Log in to see Polyclonal 2
3.0788064 ABIN1859412 ICC IHC WB Rabbit IgG AA 22-213 Log in to see Polyclonal 0
3.0788064 ABIN4291000 ELISA ICC IF IHC WB Mouse IgG1 Log in to see 1B5D12 0
3.0788064 ABIN3187774 ELISA WB Rabbit IgG Internal Region Log in to see Polyclonal 0
3.0788064 ABIN1873210 WB Rabbit IgG Log in to see Polyclonal 0
3.0788064 ABIN670371 IF (p) IHC (p) WB Rabbit IgG AA 258-268 Log in to see Polyclonal 0
3.0788064 ABIN439058 ICC IF WB Rabbit Log in to see Polyclonal 0
3.0788064 ABIN1539791 WB Mouse IgG2a Log in to see 7G7B6 0
3.0788064 ABIN498207 IF WB Rabbit Log in to see Polyclonal 0
3.0788064 ABIN2935448 IF/ICC IHC IP WB Rabbit AA 22-213 Log in to see Polyclonal 0
3.0788064 ABIN4291007 IHC IHC (p) WB Rabbit C-Term Log in to see SP176 0
3.0788064 ABIN2974449 IHC WB Rabbit IgG Log in to see Polyclonal 0
3.0788064 ABIN5580842 ELISA IF WB Rabbit Log in to see Polyclonal 0
3.0788064 ABIN2971873 WB Rabbit Center Log in to see Polyclonal 0
3.0788064 ABIN561456 ELISA WB Mouse IgG2a kappa AA 22-122, partial Log in to see 1D6 0

General

Antigen Interleukin 2 Receptor, alpha (IL2RA) Antibodies
Epitope AA 34-139
(23), (21), (18), (18), (16), (16), (15), (15), (13), (13), (8), (7), (7), (6), (5), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Monkey, Rat (Rattus)
(655), (480), (167), (22), (19), (15), (8), (6), (6), (6), (5), (4), (4), (4), (3), (1)
Host Mouse
(627), (400), (174), (12), (6), (4), (3), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD25 antibody is un-conjugated
(130), (123), (101), (82), (29), (29), (28), (24), (22), (18), (17), (15), (13), (11), (11), (11), (11), (11), (10), (10), (9), (9), (9), (9), (8), (8), (8), (6), (6), (6), (5), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(993), (223), (182), (154), (132), (127), (81), (79), (55), (30), (20), (20), (18), (17), (17), (13), (8), (8), (7), (6), (5), (4), (2), (2), (1), (1), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-CD25 Antibody

Target Details CD25 Application Details Handling References for anti-CD25 antibody (ABIN1724861) Images
Purification Purified
Immunogen Purified recombinant fragment of human IL2RA (AA 34-139) expressed in E. Coli.
Clone 1B5D12
Isotype IgG1
Plasmids, Primers & others

Target Details CD25

Product Details anti-CD25 Antibody Application Details Handling References for anti-CD25 antibody (ABIN1724861) Images back to top
Antigen
Alternative Name IL2RA (IL2RA Antibody Abstract)
Background The interleukin 2 (IL2) receptor alpha (IL2RA) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Alternately-spliced IL2RA mRNAs have been isolated, but the significance of each is presently unknown. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency. , , , ,
Molecular Weight 30.8 kDa
Gene ID 3559
HGNC 3559
Pathways JAK-STAT Signaling, Growth Factor Binding, Activated T Cell Proliferation

Application Details

Product Details anti-CD25 Antibody Target Details CD25 Handling References for anti-CD25 antibody (ABIN1724861) Images back to top
Application Notes ELISA: 1:10000, WB: 1:500 - 1:2000, IHC: 1:200 - 1:1000, ICC: 1:200 - 1:1000
Restrictions For Research Use only

Handling

Product Details anti-CD25 Antibody Target Details CD25 Application Details References for anti-CD25 antibody (ABIN1724861) Images back to top
Format Liquid
Buffer PBS with 0.05 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment 4°C, -20°C for long term storage

References for anti-CD25 antibody (ABIN1724861)

Product Details anti-CD25 Antibody Target Details CD25 Application Details Handling Images back to top
Product cited in:

Shvidel, Braester, Bairey, Rahimi-Levene, Herishanu, Tadmor, Klepfish, Ruchlemer, Shtalrid, Berrebi, Polliack: "Cell surface expression of CD25 antigen (surface IL-2 receptor α-chain) is not a prognostic marker in chronic lymphocytic leukemia: results of a retrospective study of 281 patients." in: Annals of hematology, Vol. 91, Issue 10, pp. 1597-602, 2012

Sun, Wang, Zhu, Fang, Yu, Xu: "The interleukin-2 receptor α chain (CD25) plays an important role in regulating monocyte-derived CD40 expression during anti-porcine cellular responses." in: Transplantation proceedings, Vol. 44, Issue 4, pp. 1139-42, 2012

Images

Product Details anti-CD25 Antibody Target Details CD25 Application Details Handling References for anti-CD25 antibody (ABIN1724861) back to top
Supplier Images
ELISA image for anti-Interleukin 2 Receptor, alpha (IL2RA) (AA 34-139) antibody (ABIN1724861) anti-Interleukin 2 Receptor, alpha (IL2RA) (AA 34-139) antibody
Immunocytochemistry (ICC) image for anti-Interleukin 2 Receptor, alpha (IL2RA) (AA 34-139) antibody (ABIN1724861) anti-Interleukin 2 Receptor, alpha (IL2RA) (AA 34-139) antibody (Image 2)
Western Blotting (WB) image for anti-Interleukin 2 Receptor, alpha (IL2RA) (AA 34-139) antibody (ABIN1724861) anti-Interleukin 2 Receptor, alpha (IL2RA) (AA 34-139) antibody (Image 3)
Western Blotting (WB) image for anti-Interleukin 2 Receptor, alpha (IL2RA) (AA 34-139) antibody (ABIN1724861) anti-Interleukin 2 Receptor, alpha (IL2RA) (AA 34-139) antibody (Image 4)
Western Blotting (WB) image for anti-Interleukin 2 Receptor, alpha (IL2RA) (AA 34-139) antibody (ABIN1724861) anti-Interleukin 2 Receptor, alpha (IL2RA) (AA 34-139) antibody (Image 5)
Immunohistochemistry (IHC) image for anti-Interleukin 2 Receptor, alpha (IL2RA) (AA 34-139) antibody (ABIN1724861) Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using IL2RA m...
Immunohistochemistry (IHC) image for anti-Interleukin 2 Receptor, alpha (IL2RA) (AA 34-139) antibody (ABIN1724861) Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using IL2RA ...