anti-Human CD25 antibody for Western Blotting

Recommended CD25 Antibody (supplied by: Log in to see )

Antigen

Interleukin 2 Receptor, alpha (IL2RA) Antibodies
show synonyms for this antigen

CD25
IDDM10
IL2R
TCGFR
IL2RAC
IL-2RA
Il2r
Ly-43
interleukin 2 receptor subunit alpha
interleukin-2 receptor subunit alpha-like
interleukin 2 receptor, alpha chain
IL2RA
Il2ra
LOC100222957

Epitope

AA 34-139
Alternatives

Reactivity

Human, Mouse (Murine), Monkey, Rat (Rattus)
Alternatives

630

441

143

Host

Mouse
Alternatives

592

373

167

Clonality

Conjugate

This CD25 antibody is un-conjugated
Alternatives

129

119

Application

Immunocytochemistry (ICC), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
Alternatives

934

195

165

142

118

109

Options

Supplier

Supplier Product No.

Get this product for free

Submit your validation data for this product and get a full refund. I want to validate this product

Learn more

2 references available

Available images

ELISA image for anti-Interleukin 2 Receptor, alpha (IL2RA) (AA 34-139) antibody (ABIN1724861)

Immunocytochemistry (ICC) image for anti-Interleukin 2 Receptor, alpha (IL2RA) (AA 34-139) antibody (ABIN1724861)

Western Blotting (WB) image for anti-Interleukin 2 Receptor, alpha (IL2RA) (AA 34-139) antibody (ABIN1724861)

Immunohistochemistry (IHC) image for anti-Interleukin 2 Receptor, alpha (IL2RA) (AA 34-139) antibody (ABIN1724861)

Catalog No. ABIN1724861

$ 430.10
Plus shipping costs $45.00

Quantity

Price

100 μg

$430.10

Product detail page

Relevance Score	ABIN	Application	Conjugate	Host	Isotype	Epitope	Supplier	Clonality
21.38187	ABIN5668051	BR FACS IF IHC WB		Rabbit	IgG, kappa		Log in to see	Chimeric
19.750505	ABIN4880789	ELISA ICC IHC WB		Mouse	IgG1	AA 34-139	Log in to see	1B5D12
19.750505	ABIN1845343	ELISA IF/ICC IHC WB		Mouse	IgG1	AA 34-139	Log in to see
18.38187	ABIN5668050	BR FACS IF IHC WB		Human	IgG1, kappa		Log in to see	Chimeric
16.750505	ABIN1859412	ICC IHC WB		Rabbit	IgG	AA 22-213	Log in to see	Polyclonal
16.750505	ABIN2935448	IF/ICC IHC IP WB		Rabbit		AA 22-213	Log in to see	Polyclonal
16.132376	ABIN3187774	ELISA WB		Rabbit	IgG	Internal Region	Log in to see	Polyclonal
16.132376	ABIN3017119	WB		Rabbit			Log in to see	Polyclonal
13.750505	ABIN6269767	ELISA IHC WB		Rabbit	IgG	pSer268	Log in to see	Polyclonal
12.38187	ABIN3182447	ELISA IF IHC WB		Rabbit	IgG	pSer268	Log in to see	Polyclonal
12.38187	ABIN3185170	ELISA IF WB		Rabbit	IgG	Ser537	Log in to see	Polyclonal
12.38187	ABIN5617549	ELISA IF WB		Rabbit	IgG	pSer268	Log in to see	Polyclonal
12.38187	ABIN5617550	ELISA IF WB		Rabbit	IgG	Ser268	Log in to see	Polyclonal
12.38187	ABIN933951	Inhibition ELISA WB		Mouse	IgG2a kappa		Log in to see	M912289
12.38187	ABIN2136305	IHC (p) WB		Rabbit	IgG	N-Term	Log in to see	Polyclonal
12.38187	ABIN2136300	FACS IP WB	APC	Mouse	IgG1 kappa		Log in to see	BC96
12.38187	ABIN1099588	FACS WB		Rat	IgG2b		Log in to see	YTH 906-9HL
12.38187	ABIN1099589	FACS WB	APC	Rat	IgG2b		Log in to see	YTH 906-9HL
12.38187	ABIN1099591	FACS WB	FITC	Rat	IgG2b		Log in to see	YTH 906-9HL
12.38187	ABIN1099592	FACS WB	PE	Rat	IgG2b		Log in to see	YTH 906-9HL

More product categories related to CD25 Antibodies

Top referenced anti-CD25 antibody for Western Blotting

Human Monoclonal CD25 Primary Antibody for CyTOF, FACS - ABIN4899653 (4 Publications): Voo, Peng, Guo, Fu, Li, Frappier, Wang: Functional characterization of EBV-encoded nuclear antigen 1-specific CD4+ helper and regulatory T cells elicited by in vitro peptide stimulation. in Cancer research 2005 (PubMed)
Human Polyclonal CD25 Primary Antibody for CyTOF, FACS - ABIN4899652 (4 Publications): Binder, Vögtle, Michelfelder, Müller, Illerhaus, Sundararajan, Mertelsmann, Trepel: Identification of their epitope reveals the structural basis for the mechanism of action of the immunosuppressive antibodies basiliximab and daclizumab. in Cancer research 2007 (PubMed)
Human Monoclonal CD25 Primary Antibody for ICC, IHC - ABIN1724861 (2 Publications): Shvidel, Braester, Bairey, Rahimi-Levene, Herishanu, Tadmor, Klepfish, Ruchlemer, Shtalrid, Berrebi, Polliack: Cell surface expression of CD25 antigen (surface IL-2 receptor α-chain) is not a prognostic marker in chronic lymphocytic leukemia: results of a retrospective study of 281 patients. in Annals of hematology 2012 (PubMed)

Similar anti-CD25 Antibodies

Application / Reactivity	Human	Rat (Rattus)	Mouse (Murine)	Cow (Bovine)	Sheep (Ovine)	Monkey	Rabbit	Pig (Porcine)	Chicken	Dog (Canine)	Cynomolgus	Rhesus Monkey	Water buffalo (Bubalus bubalis)	Goat	Primate	Wild boar (Sus scrofa)
Flow Cytometry (FACS)	459 antibody	96 antibody	369 antibody	19 antibody	16 antibody	1 antibody	4 antibody	4 antibody	6 antibody	8 antibody	4 antibody	4 antibody	1 antibody	1 antibody
Immunoprecipitation (IP)	32 antibody	15 antibody	81 antibody	7 antibody	6 antibody		4 antibody	2 antibody
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))	26 antibody	29 antibody	26 antibody
Enzyme Immunoassay (EIA)	1 antibody	7 antibody
Functional Studies (Func)	15 antibody	5 antibody	20 antibody	4 antibody	1 antibody			1 antibody					1 antibody	1 antibody
Immunohistochemistry (Frozen Sections) (IHC (fro))	16 antibody	34 antibody	18 antibody	4 antibody	6 antibody		1 antibody	3 antibody
Immunohistochemistry (Acetone-fixed) (IHC (af))	1 antibody
Immunohistochemistry (IHC)	102 antibody	56 antibody	75 antibody	11 antibody	5 antibody	3 antibody	2 antibody	3 antibody			2 antibody	2 antibody	1 antibody	1 antibody	1 antibody	1 antibody
ELISA	75 antibody	36 antibody	17 antibody			3 antibody			3 antibody		1 antibody				1 antibody
Immunocytochemistry (ICC)	8 antibody	9 antibody	7 antibody	1 antibody		2 antibody		1 antibody							1 antibody	1 antibody
Immunofluorescence (IF)	110 antibody	8 antibody	12 antibody								2 antibody	2 antibody			1 antibody
Western Blotting (WB)	131 antibody	47 antibody	43 antibody	3 antibody		6 antibody		2 antibody	3 antibody	2 antibody	2 antibody	2 antibody			1 antibody	1 antibody
Blocking Antibody (Inhibition)	1 antibody		16 antibody
Blocking Reagent (BR)	2 antibody	1 antibody	4 antibody				1 antibody				2 antibody	2 antibody
Neutralization (Neut)	17 antibody	2 antibody	1 antibody
Immunofluorescence (Paraffin-embedded Sections) (IF (p))	13 antibody	13 antibody	13 antibody
ELISA (Capture)	2 antibody
ELISA (Detection)	2 antibody	1 antibody	3 antibody
Intracellular Flow Cytometry (ICFC)	4 antibody		16 antibody
Immunochromatography (IC)	1 antibody
Biochemical Assay (BCA)	4 antibody		1 antibody
ELISpot (Detection) (ELISPOT (Detection))			1 antibody
Cytotoxicity Test (CyTox)			1 antibody
In vivo Studies (in vivo)			2 antibody
Blocking Peptide (BP)	4 antibody	1 antibody	1 antibody
Immunohistochemistry (Formalin-fixed Sections) (IHC (f))			5 antibody
Cytometry by Time of Flight (CyTOF)	8 antibody	2 antibody	8 antibody							1 antibody
Immunofluorescence (fixed cells) (IF/ICC)	11 antibody	6 antibody	3 antibody	2 antibody		1 antibody		1 antibody
Dot Blot (DB)			1 antibody
Immunolocalization (IL)	1 antibody
Radioimmunoassay (RIA)	1 antibody

General
Antigen	Interleukin 2 Receptor, alpha (IL2RA) Antibodies
Epitope	AA 34-139 Alternatives
	(24), (20), (15), (15), (13), (13), (12), (9), (8), (7), (7), (6), (6), (5), (4), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity	Human, Mouse (Murine), Monkey, Rat (Rattus) Alternatives
	(630), (441), (143), (22), (16), (8), (6), (6), (5), (5), (4), (4), (1), (1), (1), (1)
Host	Mouse Alternatives
	(592), (373), (167), (12), (6), (4), (3), (2)
Clonality (Clone)	Monoclonal ( )
Conjugate	This CD25 antibody is un-conjugated Alternatives
	(129), (119), (93), (82), (26), (25), (25), (21), (20), (14), (13), (12), (11), (10), (10), (10), (10), (10), (9), (9), (9), (9), (9), (6), (6), (6), (6), (5), (5), (5), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application	Immunocytochemistry (ICC), Immunohistochemistry (IHC), ELISA, Western Blotting (WB) Alternatives (934), (195), (165), (142), (118), (109), (76), (74), (40), (20), (20), (18), (17), (17), (15), (13), (8), (8), (5), (5), (4), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
	2 references available
Supplier	Log in to see
Product Details anti-CD25 Antibody Target Details CD25 Application Details Handling References for anti-CD25 antibody (ABIN1724861) Images
Purification	Purified
Immunogen	Purified recombinant fragment of human IL2RA (AA 34-139) expressed in E. Coli.
Clone	1B5D12
Isotype	IgG1
Plasmids, Primers & others
Target Details CD25 Product Details anti-CD25 Antibody Application Details Handling References for anti-CD25 antibody (ABIN1724861) Images back to top
Antigen
Alternative Name	IL2RA (IL2RA Antibody Abstract)
Background	The interleukin 2 (IL2) receptor alpha (IL2RA) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Alternately-spliced IL2RA mRNAs have been isolated, but the significance of each is presently unknown. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency. , , , ,
Molecular Weight	30.8 kDa
Gene ID	3559
HGNC	3559
Pathways	JAK-STAT Signaling, Growth Factor Binding, Activated T Cell Proliferation
Application Details Product Details anti-CD25 Antibody Target Details CD25 Handling References for anti-CD25 antibody (ABIN1724861) Images back to top
Application Notes	ELISA: 1:10000, WB: 1:500 - 1:2000, IHC: 1:200 - 1:1000, ICC: 1:200 - 1:1000
Restrictions	For Research Use only
Handling Product Details anti-CD25 Antibody Target Details CD25 Application Details References for anti-CD25 antibody (ABIN1724861) Images back to top
Format	Liquid
Buffer	PBS with 0.05 % sodium azide
Preservative	Sodium azide
Precaution of Use	This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage	4 °C,-20 °C
Storage Comment	4°C, -20°C for long term storage
References for anti-CD25 antibody (ABIN1724861) Product Details anti-CD25 Antibody Target Details CD25 Application Details Handling Images back to top
Product cited in:	Shvidel, Braester, Bairey, Rahimi-Levene, Herishanu, Tadmor, Klepfish, Ruchlemer, Shtalrid, Berrebi, Polliack: "Cell surface expression of CD25 antigen (surface IL-2 receptor α-chain) is not a prognostic marker in chronic lymphocytic leukemia: results of a retrospective study of 281 patients." in: Annals of hematology, Vol. 91, Issue 10, pp. 1597-602, 2012 Sun, Wang, Zhu, Fang, Yu, Xu: "The interleukin-2 receptor α chain (CD25) plays an important role in regulating monocyte-derived CD40 expression during anti-porcine cellular responses." in: Transplantation proceedings, Vol. 44, Issue 4, pp. 1139-42, 2012
Images Product Details anti-CD25 Antibody Target Details CD25 Application Details Handling References for anti-CD25 antibody (ABIN1724861) back to top
Supplier Images	anti-Interleukin 2 Receptor, alpha (IL2RA) (AA 34-139) antibody anti-Interleukin 2 Receptor, alpha (IL2RA) (AA 34-139) antibody (Image 2) anti-Interleukin 2 Receptor, alpha (IL2RA) (AA 34-139) antibody (Image 3) anti-Interleukin 2 Receptor, alpha (IL2RA) (AA 34-139) antibody (Image 4) anti-Interleukin 2 Receptor, alpha (IL2RA) (AA 34-139) antibody (Image 5) Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using IL2RA m... Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using IL2RA ... Images for product: anti-Interleukin 2 Receptor, alpha (IL2RA) (AA 34-139) antibody anti-Interleukin 2 Receptor, alpha (IL2RA) (AA 34-139) antibody anti-Interleukin 2 Receptor, alpha (IL2RA) (AA 34-139) antibody (Image 2) anti-Interleukin 2 Receptor, alpha (IL2RA) (AA 34-139) antibody (Image 3) anti-Interleukin 2 Receptor, alpha (IL2RA) (AA 34-139) antibody (Image 4) anti-Interleukin 2 Receptor, alpha (IL2RA) (AA 34-139) antibody (Image 5) Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using IL2RA mouse mAb with DAB staining. Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using IL2RA mouse mAb with DAB staining.