anti-Human CD25 antibody for Western Blotting

Recommended CD25 Antibody (supplied by: Log in to see )

Antigen
Interleukin 2 Receptor, alpha (IL2RA) Antibodies
  • CD25
  • IDDM10
  • IL2R
  • TCGFR
  • IL2RAC
  • IL-2RA
  • Il2r
  • Ly-43
  • interleukin 2 receptor subunit alpha
  • interleukin-2 receptor subunit alpha-like
  • interleukin 2 receptor, alpha chain
  • IL2RA
  • Il2ra
  • LOC100222957
Epitope
AA 223-272, pSer268
22
21
20
19
18
15
15
14
13
13
7
6
6
4
4
4
3
3
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
Reactivity
Human
591
480
166
22
19
15
9
8
6
6
5
4
4
4
3
1
Host
Rabbit
590
406
150
7
5
3
3
2
Clonality
Polyclonal
Conjugate
This CD25 antibody is un-conjugated
132
119
96
79
30
29
29
24
23
18
16
14
12
12
12
12
12
11
10
10
9
8
8
7
7
6
6
6
4
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunofluorescence (IF), ELISA, Western Blotting (WB)
942
189
169
156
125
88
86
83
61
29
21
21
19
17
13
12
9
8
7
6
6
5
3
2
1
1
1
1
Options
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Catalog No. ABIN1531665
$ 302.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.3318946 ABIN1532689 IF ELISA WB Rabbit IgG AA 223-272 Log in to see Polyclonal 2
3.3318946 ABIN2705770 WB Rabbit Center Log in to see Polyclonal
3.3318946 ABIN1724861 ICC IHC ELISA WB Mouse IgG1 AA 34-139 Log in to see 1B5D12 2
3.3318946 ABIN3187774 ELISA WB Rabbit IgG Internal Region Log in to see Polyclonal
3.3318946 ABIN3044139 IHC (p) WB Rabbit IgG AA 39-57, N-Term Log in to see Polyclonal
3.3318946 ABIN439058 ICC IF WB Rabbit Log in to see Polyclonal
3.3318946 ABIN1539791 WB Mouse IgG2a Log in to see 7G7B6
3.3318946 ABIN4291000 ELISA ICC IF IHC WB Mouse IgG1 Log in to see 1B5D12
3.3318946 ABIN4291007 IHC IHC (p) WB Rabbit C-Term Log in to see SP176
3.3318946 ABIN498207 IF WB Rabbit Log in to see Polyclonal
3.3318946 ABIN1873210 WB Rabbit IgG Log in to see Polyclonal
3.3318946 ABIN3031471 ELISA WB Mouse IgG2a Log in to see 7G7B6
3.3318946 ABIN1859412 ICC IHC IP WB Rabbit IgG AA 22-213 Log in to see Polyclonal
3.3318946 ABIN561456 ELISA WB Mouse IgG2a kappa AA 22-122, partial Log in to see 1D6
3.3318946 ABIN1845343 ELISA IF/ICC IHC WB Mouse IgG1 AA 34-139 Log in to see
3.3318946 ABIN3031470 IHC (p) WB Rabbit IgG N-Term Log in to see Polyclonal
3.3318946 ABIN948021 WB Mouse AA 1-272, full length Log in to see Polyclonal
3.3318946 ABIN517010 WB Rabbit AA 1-272, full length Log in to see Polyclonal
3.3318946 ABIN2426093 ELISA WB Rabbit IgG Log in to see Polyclonal
3.3318946 ABIN3017119 WB Rabbit Log in to see Polyclonal

General

Antigen Interleukin 2 Receptor, alpha (IL2RA) Antibodies
Epitope AA 223-272, pSer268
(22), (21), (20), (19), (18), (15), (15), (14), (13), (13), (7), (6), (6), (4), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity Human
(591), (480), (166), (22), (19), (15), (9), (8), (6), (6), (5), (4), (4), (4), (3), (1)
Host Rabbit
(590), (406), (150), (7), (5), (3), (3), (2)
Clonality
Polyclonal
Conjugate This CD25 antibody is un-conjugated
(132), (119), (96), (79), (30), (29), (29), (24), (23), (18), (16), (14), (12), (12), (12), (12), (12), (11), (10), (10), (9), (8), (8), (7), (7), (6), (6), (6), (4), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunofluorescence (IF), ELISA, Western Blotting (WB)
(942), (189), (169), (156), (125), (88), (86), (83), (61), (29), (21), (21), (19), (17), (13), (12), (9), (8), (7), (6), (6), (5), (3), (2), (1), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-CD25 Antibody

Target Details CD25 Application Details Handling References for anti-CD25 antibody (ABIN1531665) Images
Specificity

IL-2R alpha/CD25 (Phospho-Ser268) Antibody detects endogenous levels of IL-2R alpha/CD25 only when phosphorylated at Ser268.

PhosphorylationH:S268

Purification The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Purity > 95 %
Immunogen The antiserum was produced against synthesized peptide derived from human IL-2R alpha/CD25 around the phosphorylation site of Ser268.
Isotype IgG

Target Details CD25

Product Details anti-CD25 Antibody Application Details Handling References for anti-CD25 antibody (ABIN1531665) Images back to top
Antigen
Alternative Name IL-2R alpha/cd25 (IL2RA Antibody Abstract)
Background

Synonyms: Interleukin-2 receptor alpha chain, IL-2 receptor alpha subunit, IL-2-RA, IL2-RA, p55, TAC antigen, CD25

NCBI Gene Symbol: IL2RA

Molecular Weight 30 kDa
Gene ID 3559
OMIM 147730
UniProt P01589
Pathways JAK-STAT Signaling, Growth Factor Binding, Activated T Cell Proliferation

Application Details

Product Details anti-CD25 Antibody Target Details CD25 Handling References for anti-CD25 antibody (ABIN1531665) Images back to top
Application Notes WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:10000
Comment

Unigene-Number: Hs.231367 (NCBI Gene Symbol: IL2RA)

Restrictions For Research Use only

Handling

Product Details anti-CD25 Antibody Target Details CD25 Application Details References for anti-CD25 antibody (ABIN1531665) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Stable at -20°C for at least 1 year.
Expiry Date 12 months

References for anti-CD25 antibody (ABIN1531665)

Product Details anti-CD25 Antibody Target Details CD25 Application Details Handling Images back to top
Background publications

Bamborough, Hedgecock, Richards: "The interleukin-2 and interleukin-4 receptors studied by molecular modelling." in: Structure (London, England : 1993), Vol. 2, Issue 9, pp. 839-51, 1995

Nikaido, Shimizu, Ishida, Sabe, Teshigawara, Maeda, Uchiyama, Yodoi, Honjo: "Molecular cloning of cDNA encoding human interleukin-2 receptor." in: Nature, Vol. 311, Issue 5987, pp. 631-5, 1984

Images

Product Details anti-CD25 Antibody Target Details CD25 Application Details Handling References for anti-CD25 antibody (ABIN1531665) back to top
Supplier Images
Western Blotting (WB) image for anti-Interleukin 2 Receptor, alpha (IL2RA) (AA 223-272), (pSer268) antibody (ABIN1531665) Western blot analysis of extracts from LOVO cells, using IL-2R alpha/CD25 (Phospho-Se...
Immunofluorescence (IF) image for anti-Interleukin 2 Receptor, alpha (IL2RA) (AA 223-272), (pSer268) antibody (ABIN1531665) Immunofluorescence analysis of HeLa cells, using IL-2R alpha/CD25 (Phospho-Ser268) An...