anti-Human CD25 antibody for Western Blotting

Recommended CD25 Antibody (supplied by: Log in to see )

Antigen

Interleukin 2 Receptor, alpha (IL2RA) Antibodies
show synonyms for this antigen

CD25
IDDM10
IL2R
TCGFR
IL2RAC
IL-2RA
Il2r
Ly-43
interleukin 2 receptor subunit alpha
interleukin-2 receptor subunit alpha-like
interleukin 2 receptor, alpha chain
IL2RA
Il2ra
LOC100222957

Epitope

AA 34-139
Alternatives

Reactivity

Human, Mouse (Murine), Monkey, Rat (Rattus)
Alternatives

655

480

167

Host

Mouse
Alternatives

627

400

174

Clonality

Conjugate

This CD25 antibody is un-conjugated
Alternatives

130

123

101

Application

Immunocytochemistry (ICC), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
Alternatives

993

223

182

154

132

127

Options

Supplier

Supplier Product No.

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2 references available

Available images

ELISA image for anti-Interleukin 2 Receptor, alpha (IL2RA) (AA 34-139) antibody (ABIN1724861)

Immunocytochemistry (ICC) image for anti-Interleukin 2 Receptor, alpha (IL2RA) (AA 34-139) antibody (ABIN1724861)

Western Blotting (WB) image for anti-Interleukin 2 Receptor, alpha (IL2RA) (AA 34-139) antibody (ABIN1724861)

Immunohistochemistry (IHC) image for anti-Interleukin 2 Receptor, alpha (IL2RA) (AA 34-139) antibody (ABIN1724861)

Catalog No. ABIN1724861

$ 430.10
Plus shipping costs $45.00

Quantity

Price

100 μg

$430.10

Product detail page

Relevance Score	ABIN	Application	Host	Isotype	Epitope	Supplier	Clonality	References
3.0788064	ABIN2705770	WB	Rabbit		Center	Log in to see	Polyclonal	0
3.0788064	ABIN3044139	IHC (p) WB	Rabbit	IgG	AA 39-57, N-Term	Log in to see	Polyclonal	0
3.0788064	ABIN4880789	ELISA ICC IHC WB	Mouse	IgG1	AA 34-139	Log in to see	1B5D12	0
3.0788064	ABIN1845343	ELISA IF/ICC IHC WB	Mouse	IgG1	AA 34-139	Log in to see		0
3.0788064	ABIN1531665	IF ELISA WB	Rabbit	IgG	AA 223-272, pSer268	Log in to see	Polyclonal	2
3.0788064	ABIN1532689	IF ELISA WB	Rabbit	IgG	AA 223-272	Log in to see	Polyclonal	2
3.0788064	ABIN1859412	ICC IHC WB	Rabbit	IgG	AA 22-213	Log in to see	Polyclonal	0
3.0788064	ABIN4291000	ELISA ICC IF IHC WB	Mouse	IgG1		Log in to see	1B5D12	0
3.0788064	ABIN3187774	ELISA WB	Rabbit	IgG	Internal Region	Log in to see	Polyclonal	0
3.0788064	ABIN1873210	WB	Rabbit	IgG		Log in to see	Polyclonal	0
3.0788064	ABIN670371	IF (p) IHC (p) WB	Rabbit	IgG	AA 258-268	Log in to see	Polyclonal	0
3.0788064	ABIN439058	ICC IF WB	Rabbit			Log in to see	Polyclonal	0
3.0788064	ABIN1539791	WB	Mouse	IgG2a		Log in to see	7G7B6	0
3.0788064	ABIN498207	IF WB	Rabbit			Log in to see	Polyclonal	0
3.0788064	ABIN2935448	IF/ICC IHC IP WB	Rabbit		AA 22-213	Log in to see	Polyclonal	0
3.0788064	ABIN4291007	IHC IHC (p) WB	Rabbit		C-Term	Log in to see	SP176	0
3.0788064	ABIN2974449	IHC WB	Rabbit	IgG		Log in to see	Polyclonal	0
3.0788064	ABIN5580842	ELISA IF WB	Rabbit			Log in to see	Polyclonal	0
3.0788064	ABIN2971873	WB	Rabbit		Center	Log in to see	Polyclonal	0
3.0788064	ABIN561456	ELISA WB	Mouse	IgG2a kappa	AA 22-122, partial	Log in to see	1D6	0

More product categories related to CD25 Antibodies

Top referenced anti-CD25 antibody for Western Blotting

Human Monoclonal CD25 Primary Antibody for CyTOF, FACS - ABIN4899653 (4 Publications): Voo, Peng, Guo, Fu, Li, Frappier, Wang: Functional characterization of EBV-encoded nuclear antigen 1-specific CD4+ helper and regulatory T cells elicited by in vitro peptide stimulation. in Cancer research 2005 (PubMed)
Human Polyclonal CD25 Primary Antibody for CyTOF, FACS - ABIN4899652 (4 Publications): Binder, Vögtle, Michelfelder, Müller, Illerhaus, Sundararajan, Mertelsmann, Trepel: Identification of their epitope reveals the structural basis for the mechanism of action of the immunosuppressive antibodies basiliximab and daclizumab. in Cancer research 2007 (PubMed)
Human Monoclonal CD25 Primary Antibody for ICC, IHC - ABIN1724861 (2 Publications): Shvidel, Braester, Bairey, Rahimi-Levene, Herishanu, Tadmor, Klepfish, Ruchlemer, Shtalrid, Berrebi, Polliack: Cell surface expression of CD25 antigen (surface IL-2 receptor α-chain) is not a prognostic marker in chronic lymphocytic leukemia: results of a retrospective study of 281 patients. in Annals of hematology 2012 (PubMed)

Similar anti-CD25 Antibodies

Application / Reactivity	Human	Rat (Rattus)	Mouse (Murine)	Cow (Bovine)	Sheep (Ovine)	Monkey	Rabbit	Pig (Porcine)	Chicken	Dog (Canine)	Cynomolgus	Rhesus Monkey	Water buffalo (Bubalus bubalis)	Goat	Primate	Wild boar (Sus scrofa)
Flow Cytometry (FACS)	470 antibody	111 antibody	393 antibody	20 antibody	19 antibody	1 antibody	4 antibody	4 antibody	6 antibody	9 antibody	4 antibody	4 antibody	3 antibody	4 antibody
Immunoprecipitation (IP)	32 antibody	17 antibody	91 antibody	7 antibody	6 antibody		4 antibody	2 antibody
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))	27 antibody	31 antibody	26 antibody
Enzyme Immunoassay (EIA)	1 antibody	7 antibody
Functional Studies (Func)	13 antibody	5 antibody	24 antibody	5 antibody	4 antibody			1 antibody					3 antibody	4 antibody
Immunohistochemistry (Frozen Sections) (IHC (fro))	16 antibody	36 antibody	22 antibody	4 antibody	6 antibody		1 antibody	3 antibody
Immunohistochemistry (Acetone-fixed) (IHC (af))	1 antibody
Immunohistochemistry (IHC)	115 antibody	72 antibody	96 antibody	12 antibody	8 antibody	3 antibody	2 antibody	3 antibody			2 antibody	2 antibody	3 antibody	4 antibody	15 antibody	1 antibody
ELISA	88 antibody	51 antibody	29 antibody			3 antibody			3 antibody		1 antibody				15 antibody
Immunocytochemistry (ICC)	22 antibody	21 antibody	19 antibody	1 antibody		2 antibody		1 antibody							14 antibody	1 antibody
Immunofluorescence (IF)	121 antibody	20 antibody	24 antibody								2 antibody	2 antibody			14 antibody
Western Blotting (WB)	146 antibody	60 antibody	55 antibody	3 antibody		6 antibody		2 antibody	3 antibody	2 antibody	2 antibody	2 antibody			14 antibody	1 antibody
Blocking Antibody (Inhibition)	1 antibody		16 antibody
Blocking Reagent (BR)	2 antibody	1 antibody	4 antibody				1 antibody				2 antibody	2 antibody
Neutralization (Neut)	16 antibody	2 antibody	1 antibody
Immunofluorescence (Paraffin-embedded Sections) (IF (p))	13 antibody	13 antibody	13 antibody
ELISA (Capture)	2 antibody
ELISA (Detection)	2 antibody	1 antibody	3 antibody
Intracellular Flow Cytometry (ICFC)	4 antibody		16 antibody
Immunochromatography (IC)	1 antibody
Biochemical Assay (BCA)	4 antibody		1 antibody
ELISpot (Detection) (ELISPOT (Detection))			1 antibody
Cytotoxicity Test (CyTox)			1 antibody
In vivo Studies (in vivo)			2 antibody
Blocking Peptide (BP)	4 antibody	1 antibody	1 antibody
Immunohistochemistry (Formalin-fixed Sections) (IHC (f))			5 antibody
Cytometry by Time of Flight (CyTOF)	7 antibody	2 antibody	8 antibody							1 antibody
Immunofluorescence (fixed cells) (IF/ICC)	11 antibody	6 antibody	3 antibody	2 antibody		1 antibody		1 antibody
Dot Blot (DB)			1 antibody

General
Antigen	Interleukin 2 Receptor, alpha (IL2RA) Antibodies
Epitope	AA 34-139 Alternatives
	(23), (21), (18), (18), (16), (16), (15), (15), (13), (13), (8), (7), (7), (6), (5), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity	Human, Mouse (Murine), Monkey, Rat (Rattus) Alternatives
	(655), (480), (167), (22), (19), (15), (8), (6), (6), (6), (5), (4), (4), (4), (3), (1)
Host	Mouse Alternatives
	(627), (400), (174), (12), (6), (4), (3), (2)
Clonality (Clone)	Monoclonal ( )
Conjugate	This CD25 antibody is un-conjugated Alternatives
	(130), (123), (101), (82), (29), (29), (28), (24), (22), (18), (17), (15), (13), (11), (11), (11), (11), (11), (10), (10), (9), (9), (9), (9), (8), (8), (8), (6), (6), (6), (5), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application	Immunocytochemistry (ICC), Immunohistochemistry (IHC), ELISA, Western Blotting (WB) Alternatives (993), (223), (182), (154), (132), (127), (81), (79), (55), (30), (20), (20), (18), (17), (17), (13), (8), (8), (7), (6), (5), (4), (2), (2), (1), (1), (1), (1), (1)
	2 references available
Supplier	Log in to see
Product Details anti-CD25 Antibody Target Details CD25 Application Details Handling References for anti-CD25 antibody (ABIN1724861) Images
Purification	Purified
Immunogen	Purified recombinant fragment of human IL2RA (AA 34-139) expressed in E. Coli.
Clone	1B5D12
Isotype	IgG1
Plasmids, Primers & others
Target Details CD25 Product Details anti-CD25 Antibody Application Details Handling References for anti-CD25 antibody (ABIN1724861) Images back to top
Antigen
Alternative Name	IL2RA (IL2RA Antibody Abstract)
Background	The interleukin 2 (IL2) receptor alpha (IL2RA) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Alternately-spliced IL2RA mRNAs have been isolated, but the significance of each is presently unknown. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency. , , , ,
Molecular Weight	30.8 kDa
Gene ID	3559
HGNC	3559
Pathways	JAK-STAT Signaling, Growth Factor Binding, Activated T Cell Proliferation
Application Details Product Details anti-CD25 Antibody Target Details CD25 Handling References for anti-CD25 antibody (ABIN1724861) Images back to top
Application Notes	ELISA: 1:10000, WB: 1:500 - 1:2000, IHC: 1:200 - 1:1000, ICC: 1:200 - 1:1000
Restrictions	For Research Use only
Handling Product Details anti-CD25 Antibody Target Details CD25 Application Details References for anti-CD25 antibody (ABIN1724861) Images back to top
Format	Liquid
Buffer	PBS with 0.05 % sodium azide
Preservative	Sodium azide
Precaution of Use	This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage	4 °C,-20 °C
Storage Comment	4°C, -20°C for long term storage
References for anti-CD25 antibody (ABIN1724861) Product Details anti-CD25 Antibody Target Details CD25 Application Details Handling Images back to top
Product cited in:	Shvidel, Braester, Bairey, Rahimi-Levene, Herishanu, Tadmor, Klepfish, Ruchlemer, Shtalrid, Berrebi, Polliack: "Cell surface expression of CD25 antigen (surface IL-2 receptor α-chain) is not a prognostic marker in chronic lymphocytic leukemia: results of a retrospective study of 281 patients." in: Annals of hematology, Vol. 91, Issue 10, pp. 1597-602, 2012 Sun, Wang, Zhu, Fang, Yu, Xu: "The interleukin-2 receptor α chain (CD25) plays an important role in regulating monocyte-derived CD40 expression during anti-porcine cellular responses." in: Transplantation proceedings, Vol. 44, Issue 4, pp. 1139-42, 2012
Images Product Details anti-CD25 Antibody Target Details CD25 Application Details Handling References for anti-CD25 antibody (ABIN1724861) back to top
Supplier Images	anti-Interleukin 2 Receptor, alpha (IL2RA) (AA 34-139) antibody anti-Interleukin 2 Receptor, alpha (IL2RA) (AA 34-139) antibody (Image 2) anti-Interleukin 2 Receptor, alpha (IL2RA) (AA 34-139) antibody (Image 3) anti-Interleukin 2 Receptor, alpha (IL2RA) (AA 34-139) antibody (Image 4) anti-Interleukin 2 Receptor, alpha (IL2RA) (AA 34-139) antibody (Image 5) Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using IL2RA m... Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using IL2RA ... Images for product: anti-Interleukin 2 Receptor, alpha (IL2RA) (AA 34-139) antibody anti-Interleukin 2 Receptor, alpha (IL2RA) (AA 34-139) antibody anti-Interleukin 2 Receptor, alpha (IL2RA) (AA 34-139) antibody (Image 2) anti-Interleukin 2 Receptor, alpha (IL2RA) (AA 34-139) antibody (Image 3) anti-Interleukin 2 Receptor, alpha (IL2RA) (AA 34-139) antibody (Image 4) anti-Interleukin 2 Receptor, alpha (IL2RA) (AA 34-139) antibody (Image 5) Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using IL2RA mouse mAb with DAB staining. Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using IL2RA mouse mAb with DAB staining.