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|Antigen||Colony Stimulating Factor 2 Receptor, Beta, Low-Affinity (Granulocyte-Macrophage) (CSF2RB) Antibodies|
|Conjugate||This CSF2RB antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Western Blotting (WB)
|5 references available|
|Supplier||Log in to see|
Product Details anti-CSF2RB AntibodyTarget Details CSF2RB Application Details Handling References for anti-CSF2RB antibody (ABIN358147) Images
|Specificity||This antibody detects IL3R pTyr766.|
|Purification||Protein G Affinity Chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.|
|Immunogen||This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Y766 of human IL3R.|
Target Details CSF2RBProduct Details anti-CSF2RB Antibody Application Details Handling References for anti-CSF2RB antibody (ABIN358147) Images back to top
|Alternative Name||CD131 / CSF2RB (CSF2RB Antibody Abstract)|
|Background||IL3R (also known as CSF2RB) is a common beta chain of the high affinity receptor for IL-3, IL-5 and GM-CSF. Defective IL3R has been reported to be associated with protein alveolar proteinosis (PAP). PAP is an autosomal recessive fatal respiratory disease.Synonyms: Cytokine receptor common subunit beta, GM-CSF-RB, GM-CSF/IL-3/IL-5 receptor common beta-chain, IL3RB, IL5RB|
|Molecular Weight||97336 Da|
|Gene ID||1439, 9606|
Application DetailsProduct Details anti-CSF2RB Antibody Target Details CSF2RB Handling References for anti-CSF2RB antibody (ABIN358147) Images back to top
ELISA: 1/1,000. Dot Blot: 1/500. Western Blot: 1/100-1/500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-CSF2RB Antibody Target Details CSF2RB Application Details References for anti-CSF2RB antibody (ABIN358147) Images back to top
|Buffer||PBS with 0.09 % (W/V) Sodium Azide as preservative.|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.|
References for anti-CSF2RB antibody (ABIN358147)Product Details anti-CSF2RB Antibody Target Details CSF2RB Application Details Handling Images back to top
Murphy, Ford, Olsen, Gustin, Jeffrey, Ollis, Young: "Interleukin-3 binding to the murine betaIL-3 and human betac receptors involves functional epitopes formed by domains 1 and 4 of different protein chains." in: The Journal of biological chemistry, Vol. 279, Issue 25, pp. 26500-8, 2004
McClure, Hercus, Cambareri, Woodcock, Bagley, Howlett, Lopez: "Molecular assembly of the ternary granulocyte-macrophage colony-stimulating factor receptor complex." in: Blood, Vol. 101, Issue 4, pp. 1308-15, 2003
Scibek, Evergren, Zahn, Canziani, Van Ryk, Chaiken: "Biosensor analysis of dynamics of interleukin 5 receptor subunit beta(c) interaction with IL5:IL5R(alpha) complexes." in: Analytical biochemistry, Vol. 307, Issue 2, pp. 258-65, 2002
Blake, Jenkins, DAndrea, Gonda: "Functional cross-talk between cytokine receptors revealed by activating mutations in the extracellular domain of the beta-subunit of the GM-CSF receptor." in: Journal of leukocyte biology, Vol. 72, Issue 6, pp. 1246-55, 2002
Evans, Ariffin, Pierce, Whetton: "Identification of primary structural features that define the differential actions of IL-3 and GM-CSF receptors." in: Blood, Vol. 100, Issue 9, pp. 3164-74, 2002
ImagesProduct Details anti-CSF2RB Antibody Target Details CSF2RB Application Details Handling References for anti-CSF2RB antibody (ABIN358147) back to top