|Antigen||Interleukin 13 (IL13) Antibodies|
|Reactivity||Pig (Porcine) Alternatives|
|Conjugate||This IL-13 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Western Blotting (WB)
|1 reference available|
|Supplier||Log in to see|
Product Details anti-IL-13 AntibodyTarget Details IL-13 Application Details Handling References for anti-IL-13 antibody (ABIN401064) Images
|Specificity||This antibody reacts to Interleukin-13.|
Species reactivity (expected):Sheep, Dolphin, Beluga, Bovine, Human, Certain monkey, Dusky titi, Macaque, Baboon, Mangabey, Canine, Llama, Camel, Galago, Horse, Rat, Mouse, Gerbil.
Species reactivity (tested):Porcine.
|Purification||Affinity chromatography on Protein A|
|Immunogen||Full length recombinant protein raised in yeast, corresponding to mature swine IL-13 protein|
Target Details IL-13Product Details anti-IL-13 Antibody Application Details Handling References for anti-IL-13 antibody (ABIN401064) Images back to top
|Alternative Name||Interleukin-13 / IL13 (IL13 Antibody Abstract)|
|Background||Interleukin 13 (IL-13), of the IL-4 superfamily, is a cytokine secreted by many cell types, but especially T helper type 2 (Th2) cells, that is an important mediator of allergic inflammation and disease. IL-13 induces its effects through a multi-subunit receptor that includes the alpha chain of the IL-4 receptor (IL-4Ra) and at least one IL-13-specific binding chain. Most of the biological effects of IL-13, like those of IL-4, are linked to a single transcription factor, STAT6. In humans, IL-13 can induce immunoglobulin E (IgE) secretion from activated B cells. In mice, deletion of IL-13 does not markedly affect either Th2 cell development or antigen-specific IgE responses induced by potent allergens. Deletion of IL-4 abrogates these responses. IL-13 acts as a molecular bridge linking allergic inflammatory cells to the non-immune cells in contact with them, thus altering physiological function. Although IL-13 is associated primarily with the induction of airway disease, including airway hyperresponsiveness, goblet cell metaplasia and mucus hypersecretion, it also induces airway matrix metalloproteinases as part of a mechanism that protects against excessive allergic inflammation that predisposes to asphyxiation.Synonyms: IL-13, NC30|
|Pathways||JAK-STAT Signaling, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport|
Application DetailsProduct Details anti-IL-13 Antibody Target Details IL-13 Handling References for anti-IL-13 antibody (ABIN401064) Images back to top
ELISA: 1/11,000. Western Blot: 1.5 - 2 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-IL-13 Antibody Target Details IL-13 Application Details References for anti-IL-13 antibody (ABIN401064) Images back to top
|Reconstitution||Restore with 100 μL of deionized water or equivalent.|
|Concentration||1.0 mg/mL by UV absorbance at 280 nm|
|Buffer||0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.|
References for anti-IL-13 antibody (ABIN401064)Product Details anti-IL-13 Antibody Target Details IL-13 Application Details Handling Images back to top
Trigona, Brown, Estes: "Functional implications for signaling via the IL4R/IL13R complex on bovine cells." in: Veterinary immunology and immunopathology, Vol. 72, Issue 1-2, pp. 73-9, 2000
ImagesProduct Details anti-IL-13 Antibody Target Details IL-13 Application Details Handling References for anti-IL-13 antibody (ABIN401064) back to top