|Antigen||Interleukin 21 (IL21) Antibodies|
|Epitope||AA 24-152 Alternatives|
|Reactivity||Cow (Bovine) Alternatives|
|Conjugate||This IL21 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Western Blotting (WB)
|1 reference available|
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Product Details anti-IL21 AntibodyTarget Details IL21 Application Details Handling References for anti-IL21 antibody (ABIN401062) Images
|Specificity||This antibody reacts to Interleukin-21.|
Species reactivity (expected):Porcine, Horse, Human, Canine, Macaque, Platypus and Syrian hamster, Mouse, Rat, Chicken.
Species reactivity (tested):Bovine.
|Purification||Affinity chromatography on Protein A|
|Immunogen||Recombinant protein raised in yeast, corresponding to amino acid residues 24-152 of bovine IL-21 protein|
Target Details IL21Product Details anti-IL21 Antibody Application Details Handling References for anti-IL21 antibody (ABIN401062) Images back to top
|Alternative Name||Interleukin-21 / IL21 (IL21 Antibody Abstract)|
|Background||Interleukin-21 (IL-21) is a secreted, type-I cytokine with immunoregulatory activity. Human IL-21 shares the common ?-chain with IL-2, IL-4, IL-7, IL-9, and IL-15 proteins but, in addition, binds to a unique IL-21Ra chain which triggers a cascade of events which includes activation of the tyrosine kinases JAK1 and JAK3, followed by activation of the transcription factors STAT1 and STAT3. Bovine and human IL-21 have pleiotropic functions and are mainly produced by activated T-cells in response to antigenic stimulation, but target a broad range of lymphoid and myeloid cells of the immune system (T cells, B cells, natural killer (NK) cells and dendritic cells). IL21 is therefore is able to regulate innate and acquired immune responses. The biological effects of IL-21 include induction of differentiation, maturation, and proliferation of T-cells-stimulated B-cells into plasma cells and memory B-cells, stimulation (in conjunction) with IL-4 of IgG production, and induction of apoptotic effects in naive B-cells and stimulated B-cells in the absence of T-cell signaling. Human IL-21 has also been shown to promote the anti-tumor activity of CD8+ T-cells and NK cells. During T-cell mediated immune response, IL21 may inhibit dendritic cells' (DC) activation and maturation. In synergy with IL15 and IL18, IL21 stimulates interferon gamma production in T-cells and NK cells, with the IL15, it may play a role in proliferation and maturation of natural killer (NK) cells. The open reading frame of the bovine IL-21 cDNA is 459 bp in length and encodes 152 amino acids. The predicted amino acid sequence is 78-81 % and 58-67 % homologous to the predicted human and murine IL-21 amino acid sequences, respectively. In one study, recombinant bovine IL-21 strongly induced NK cell proliferation using a human NK cell-line, NK0, and enhanced the lymphokine activated killer (LAK) activity of bovine peripheral blood mononuclear cells. In another by the same authors, recombinant bovine mature IL-21 induced the proliferation of human IL-2-dependent cells, ILT-MATSynonyms: IL-21, Za11|
|Pathways||JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response|
Application DetailsProduct Details anti-IL21 Antibody Target Details IL21 Handling References for anti-IL21 antibody (ABIN401062) Images back to top
ELISA: 1/6,000. Western Blot: 1 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-IL21 Antibody Target Details IL21 Application Details References for anti-IL21 antibody (ABIN401062) Images back to top
|Concentration||1.0 mg/mL by UV absorbance at 280 nm|
|Buffer||0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.|
References for anti-IL21 antibody (ABIN401062)Product Details anti-IL21 Antibody Target Details IL21 Application Details Handling Images back to top
Muneta, Kikuma, Yoshihara, Mori: "Cloning, expression, and tissue distribution of bovine interleukin-21." in: Veterinary immunology and immunopathology, Vol. 95, Issue 1-2, pp. 73-80, 2003
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