anti-Mouse (Murine) IL7R antibody for Western Blotting

Recommended IL7R Antibody (supplied by: Log in to see )

Antigen
Interleukin 7 Receptor (IL7R) Antibodies
  • IL7R
  • CD127
  • IL-7Ralpha
  • CDW127
  • IL-7R-alpha
  • IL7RA
  • ILRA
  • IL-7RA
  • interleukin 7 receptor
  • IL7R
  • Il7r
Epitope
pTyr449
28
27
15
15
13
8
8
7
7
6
6
5
5
3
3
3
3
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Mouse (Murine)
236
207
48
5
2
2
1
Host
Rabbit
143
138
91
17
7
4
1
Clonality
Polyclonal
Conjugate
This IL7R antibody is un-conjugated
30
28
24
19
16
14
12
7
7
7
7
6
6
5
5
5
4
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
1
1
Application
Immunoprecipitation (IP), ELISA, Western Blotting (WB)
179
175
81
73
45
26
19
12
12
11
9
8
5
4
3
3
2
2
2
1
1
1
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

Submit your validation data for this product and get a full refund. I want to validate this product

Learn more

Available images

Catalog No. ABIN233847
$ 583.50
Plus shipping costs $45.00 and $23.00 dry ice
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
22.575909 ABIN5663842 IHC WB Rabbit IgG Log in to see Polyclonal 0
19.575909 ABIN3185181 ELISA WB Rabbit IgG Ser552 Log in to see Polyclonal 0
13 ABIN1176855 BR FACS IP IHC WB Rat IgG2a, kappa Log in to see SB-14 9
12.778215 ABIN768570 ELISA WB Goat Internal Region Log in to see Polyclonal 0
12.778215 ABIN3182449 ELISA IF WB Rabbit IgG pTyr449 Log in to see Polyclonal 0
10.797692 ABIN6262569 ELISA ICC IF IHC WB Rabbit IgG Log in to see Polyclonal 0
7.7976923 ABIN1531668 IF ELISA WB Rabbit IgG AA 410-459, pTyr449 Log in to see Polyclonal 0
7.7976923 ABIN1175191 ICC IHC WB Rabbit IgG AA 48-217 Log in to see Polyclonal 0
7.7976923 ABIN801438 IF (p) IHC (p) WB Rabbit IgG AA 440-451, pTyr449 Log in to see Polyclonal 0
7.7976923 ABIN2935550 IF/ICC IHC IP WB Rabbit AA 48-217 Log in to see Polyclonal 0
7 ABIN351553 ELISA IHC IHC (p) WB Rabbit pTyr449 Log in to see Polyclonal 0
7 ABIN351552 ELISA IHC IHC (p) WB Rabbit C-Term Log in to see Polyclonal 0
7 ABIN2662995 ELISA (Detection) WB PE Rat IgG2a kappa Log in to see A7R34 6
5.5 ABIN4899576 CyTOF FACS WB Goat IgG AA 21-239 Log in to see Polyclonal 2
4.7976923 ABIN2957844 WB Rabbit C-Term Log in to see Polyclonal 0
4.7976923 ABIN1532692 ELISA WB Rabbit IgG AA 410-459 Log in to see Polyclonal 1
4.7976923 ABIN2464495 ELISA WB Goat Internal Region Log in to see Polyclonal 0
4.7976923 ABIN5958590 ELISA WB Rabbit IgG Tyr449 Log in to see Polyclonal 0
4.7976923 ABIN5964881 WB Rabbit IgG Log in to see Polyclonal 0
4.7976923 ABIN2935551 ELISA WB Rabbit IgG Log in to see Polyclonal 0

General

Antigen Interleukin 7 Receptor (IL7R) Antibodies
Epitope pTyr449
(28), (27), (15), (15), (13), (8), (8), (7), (7), (6), (6), (5), (5), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Mouse (Murine)
(236), (207), (48), (5), (2), (2), (1)
Host Rabbit
(143), (138), (91), (17), (7), (4), (1)
Clonality
Polyclonal
Conjugate This IL7R antibody is un-conjugated
(30), (28), (24), (19), (16), (14), (12), (7), (7), (7), (7), (6), (6), (5), (5), (5), (4), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1)
Application Immunoprecipitation (IP), ELISA, Western Blotting (WB)
(179), (175), (81), (73), (45), (26), (19), (12), (12), (11), (9), (8), (5), (4), (3), (3), (2), (2), (2), (1), (1), (1)
Pubmed 1 reference available
Supplier Log in to see

Product Details anti-IL7R Antibody

Target Details IL7R Application Details Handling References for anti-IL7R antibody (ABIN233847) Images
Cross-Reactivity Mouse (Murine), Rat (Rattus)
Characteristics Concentration Definition: by UV absorbance at 280 nm
Immunogen This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to residues near Y449 of mouse IL-7 protein.
Isotype IgG
Plasmids, Primers & others

Target Details IL7R

Product Details anti-IL7R Antibody Application Details Handling References for anti-IL7R antibody (ABIN233847) Images back to top
Antigen
Alternative Name IL-7 Receptor Alpha Chain (IL7R Antibody Abstract)
Background This antibody is designed, produced, and is suitable for Cancer, Immunology and Nuclear Signaling research. Interleukin-7 is a glycoprotein involved in the regulation of lymphopoiesis. Response of cells to IL7 is dependent on the presence of the interleukin 7 receptor (IL7R); the active receptor is an alpha/gamma chain heterodimer. The gamma(c) chain, which also associates with the interleukin-2 receptor, serves primarily to activate signal transduction by the IL7R complex, while the alpha chain of IL7R determines specific signaling events through its association with cytoplasmic signaling molecules.  The human and mouse sequence is nearly identical.  In humans, severe combined immunodeficiency is caused by genetic defects in IL-7 receptor. The tyrosine residue at the 449 position is a critical signaling site of the intracellular domain of IL-7 receptor. This site is rapidly phosphorylated by janus kinases after the IL-7 receptor is engaged. IL-7 Receptor Alpha Chain phospho Y449 Antibody is ideal for investigators involved in Cell Signaling, Signal Transduction and Immunology research.
Synonyms: IL7Ralpha antibody, Interleukin 7 receptor alpha chain antibody, Interleukin 7 receptor antibody
Gene ID 16197, 45269152
UniProt P16872
Pathways JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Regulation of Cell Size

Application Details

Product Details anti-IL7R Antibody Target Details IL7R Handling References for anti-IL7R antibody (ABIN233847) Images back to top
Application Notes This affinity purified antibody has been tested for use in ELISA, immunoprecipitation, and western blotting.  Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 55 kDa in size corresponding to phosphorylated IL-7 by western blotting in the appropriate cell lysate or extract.  This phospho-specific polyclonal antibody reacts with mouse IL-7 pY449 and shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide.
Restrictions For Research Use only

Handling

Product Details anti-IL7R Antibody Target Details IL7R Application Details References for anti-IL7R antibody (ABIN233847) Images back to top
Format Liquid
Concentration 0.82 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C

References for anti-IL7R antibody (ABIN233847)

Product Details anti-IL7R Antibody Target Details IL7R Application Details Handling Images back to top
Product cited in:

Osborne, Dhanji, Snow, Priatel, Ma, Miners, Teh, Goldsmith, Abraham: "Impaired CD8 T cell memory and CD4 T cell primary responses in IL-7R alpha mutant mice." in: The Journal of experimental medicine, Vol. 204, Issue 3, pp. 619-31, 2007

Images

Product Details anti-IL7R Antibody Target Details IL7R Application Details Handling References for anti-IL7R antibody (ABIN233847) back to top
Supplier Images
 image for anti-Interleukin 7 Receptor (IL7R) (pTyr449) antibody (ABIN233847) anti-Interleukin 7 Receptor (IL7R) (pTyr449) antibody
Western Blotting (WB) image for anti-Interleukin 7 Receptor (IL7R) (pTyr449) antibody (ABIN233847) Western Blot and immunoprecipitation of Rabbit anti- IL-7-Receptor-alpha-chain-pY449 ...
Western Blotting (WB) image for anti-Interleukin 7 Receptor (IL7R) (pTyr449) antibody (ABIN233847) Western Blot of Rabbit anti- IL-7-Receptor-alpha-chain-pY449 antibody. Load: thymocyt...