anti-Mouse (Murine) IL7R antibody for Western Blotting

Recommended IL7R Antibody (supplied by: Log in to see )

Antigen
Interleukin 7 Receptor (IL7R) Antibodies
  • IL7R
  • CD127
  • IL-7Ralpha
  • CDW127
  • IL-7R-alpha
  • IL7RA
  • ILRA
  • IL-7RA
  • interleukin 7 receptor
  • IL7R
  • Il7r
Epitope
pTyr449
28
27
18
15
15
13
10
8
7
7
7
6
5
5
3
3
3
3
2
2
2
2
1
1
1
1
1
1
1
Reactivity
Mouse (Murine)
263
234
48
5
2
1
1
Host
Rabbit
163
144
117
18
7
4
1
Clonality
Polyclonal
Conjugate
This IL7R antibody is un-conjugated
32
30
28
23
18
17
13
12
9
9
9
7
6
6
6
6
5
5
5
5
5
5
5
4
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
Application
Immunoprecipitation (IP), ELISA, Western Blotting (WB)
221
200
83
77
49
26
18
13
12
12
9
8
5
4
3
3
2
2
2
1
1
1
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Catalog No. ABIN233847
$ 389.00
Plus shipping costs $45.00 and $23.00 dry ice
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.0845742 ABIN2957844 WB Rabbit C-Term Log in to see Polyclonal 0
3.0845742 ABIN1532692 ELISA WB Rabbit IgG AA 410-459 Log in to see Polyclonal 1
3.0845742 ABIN2464495 ELISA WB Goat Internal Region Log in to see Polyclonal 0
3.0845742 ABIN1531668 IF ELISA WB Rabbit IgG AA 410-459, pTyr449 Log in to see Polyclonal 0
3.0845742 ABIN4325440 ELISA WB Goat N-Term Log in to see Polyclonal 1
3.0845742 ABIN314654 WB Rabbit Log in to see Polyclonal 0
3.0845742 ABIN1175191 ICC IHC WB Rabbit IgG AA 48-217 Log in to see Polyclonal 0
3.0845742 ABIN801438 IF (p) IHC (p) WB Rabbit IgG AA 440-451, pTyr449 Log in to see Polyclonal 0
3.0845742 ABIN3185181 ELISA WB Rabbit IgG Ser552 Log in to see Polyclonal 0
3.0845742 ABIN259910 ELISA IHC IP WB Rabbit pTyr449 Log in to see Polyclonal 0
3.0845742 ABIN5964881 WB Rabbit IgG Log in to see Polyclonal 0
3.0845742 ABIN5663842 IHC WB Rabbit IgG Log in to see Polyclonal 0
3.0845742 ABIN4226089 WB Rat IgG kappa Log in to see 73N4E10 0
3.0845742 ABIN4325453 IHC IHC (p) WB Rat IgG2a kappa Log in to see 73N8F2 0
3.0845742 ABIN2935551 ELISA WB Rabbit IgG Log in to see Polyclonal 0
3.0845742 ABIN4325449 WB Rat IgG kappa Log in to see 73N4E10 0
3.0845742 ABIN4325451 IHC IHC (p) WB Rat IgG2a kappa Log in to see 73N8F2 0
3.0845742 ABIN2561663 ELISA WB Goat IgG Internal Region Log in to see Polyclonal 0
3.0845742 ABIN2935550 IF/ICC IHC IP WB Rabbit AA 48-217 Log in to see Polyclonal 0
1 ABIN1176855 BR FACS IP IHC WB Rat IgG2a, kappa Log in to see SB-14 9

General

Antigen Interleukin 7 Receptor (IL7R) Antibodies
Epitope pTyr449
(28), (27), (18), (15), (15), (13), (10), (8), (7), (7), (7), (6), (5), (5), (3), (3), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity Mouse (Murine)
(263), (234), (48), (5), (2), (1), (1)
Host Rabbit
(163), (144), (117), (18), (7), (4), (1)
Clonality
Polyclonal
Conjugate This IL7R antibody is un-conjugated
(32), (30), (28), (23), (18), (17), (13), (12), (9), (9), (9), (7), (6), (6), (6), (6), (5), (5), (5), (5), (5), (5), (5), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Application Immunoprecipitation (IP), ELISA, Western Blotting (WB)
(221), (200), (83), (77), (49), (26), (18), (13), (12), (12), (9), (8), (5), (4), (3), (3), (2), (2), (2), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-IL7R Antibody

Target Details IL7R Application Details Handling References for anti-IL7R antibody (ABIN233847) Images
Cross-Reactivity Mouse (Murine), Rat (Rattus)
Characteristics Concentration Definition: by UV absorbance at 280 nm
Immunogen This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to residues near Y449 of mouse IL-7 protein.
Isotype IgG
Plasmids, Primers & others

Target Details IL7R

Product Details anti-IL7R Antibody Application Details Handling References for anti-IL7R antibody (ABIN233847) Images back to top
Antigen
Alternative Name IL-7 Receptor Alpha Chain (IL7R Antibody Abstract)
Background This antibody is designed, produced, and is suitable for Cancer, Immunology and Nuclear Signaling research. Interleukin-7 is a glycoprotein involved in the regulation of lymphopoiesis. Response of cells to IL7 is dependent on the presence of the interleukin 7 receptor (IL7R); the active receptor is an alpha/gamma chain heterodimer. The gamma(c) chain, which also associates with the interleukin-2 receptor, serves primarily to activate signal transduction by the IL7R complex, while the alpha chain of IL7R determines specific signaling events through its association with cytoplasmic signaling molecules.  The human and mouse sequence is nearly identical.  In humans, severe combined immunodeficiency is caused by genetic defects in IL-7 receptor. The tyrosine residue at the 449 position is a critical signaling site of the intracellular domain of IL-7 receptor. This site is rapidly phosphorylated by janus kinases after the IL-7 receptor is engaged. IL-7 Receptor Alpha Chain phospho Y449 Antibody is ideal for investigators involved in Cell Signaling, Signal Transduction and Immunology research.
Synonyms: IL7Ralpha antibody, Interleukin 7 receptor alpha chain antibody, Interleukin 7 receptor antibody
Gene ID 16197, 45269152
UniProt P16872
Pathways JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Regulation of Cell Size

Application Details

Product Details anti-IL7R Antibody Target Details IL7R Handling References for anti-IL7R antibody (ABIN233847) Images back to top
Application Notes This affinity purified antibody has been tested for use in ELISA, immunoprecipitation, and western blotting.  Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 55 kDa in size corresponding to phosphorylated IL-7 by western blotting in the appropriate cell lysate or extract.  This phospho-specific polyclonal antibody reacts with mouse IL-7 pY449 and shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide.
Restrictions For Research Use only

Handling

Product Details anti-IL7R Antibody Target Details IL7R Application Details References for anti-IL7R antibody (ABIN233847) Images back to top
Format Liquid
Concentration 0.82 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C

References for anti-IL7R antibody (ABIN233847)

Product Details anti-IL7R Antibody Target Details IL7R Application Details Handling Images back to top
Product cited in:

Osborne, Dhanji, Snow, Priatel, Ma, Miners, Teh, Goldsmith, Abraham: "Impaired CD8 T cell memory and CD4 T cell primary responses in IL-7R alpha mutant mice." in: The Journal of experimental medicine, Vol. 204, Issue 3, pp. 619-31, 2007

Images

Product Details anti-IL7R Antibody Target Details IL7R Application Details Handling References for anti-IL7R antibody (ABIN233847) back to top
Supplier Images
Western Blotting (WB) image for anti-Interleukin 7 Receptor (IL7R) (pTyr449) antibody (ABIN233847) Western Blot and immunoprecipitation of Rabbit anti- IL-7-Receptor-alpha-chain-pY449 ...
 image for anti-Interleukin 7 Receptor (IL7R) (pTyr449) antibody (ABIN233847) anti-Interleukin 7 Receptor (IL7R) (pTyr449) antibody (Image 2)
Western Blotting (WB) image for anti-Interleukin 7 Receptor (IL7R) (pTyr449) antibody (ABIN233847) Western Blot and immunoprecipitation of Rabbit anti- IL-7-Receptor-alpha-chain-pY449 ...
Western Blotting (WB) image for anti-Interleukin 7 Receptor (IL7R) (pTyr449) antibody (ABIN233847) Western Blot of Rabbit anti- IL-7-Receptor-alpha-chain-pY449 antibody. Load: thymocyt...