anti-Human Leptin Receptor antibody for Cytometry by Time of Flight

Recommended Leptin Receptor Antibody (supplied by: Log in to see )

Antigen
Leptin Receptor (LEPR) Antibodies
  • CD295
  • LEP-R
  • LEPRD
  • OB-R
  • OBR
  • obr
  • cd295
  • ob-rb
  • Fa
  • LEPROT
  • Leprb
  • Modb1
  • OB-RGRP
  • Obr
  • db
  • diabetes
  • obese-like
  • obl
  • ObRb
  • Ob-R
  • Ob-RM
  • lepr
  • leptin receptor
  • leptin receptor S homeolog
  • LEPR
  • lepr
  • Lepr
  • lepr.S
Epitope
AA 22-839
24
18
16
15
15
15
12
6
5
5
5
5
5
4
4
4
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
221
96
64
15
7
3
3
1
1
1
1
1
1
Host
Mouse
148
67
25
16
2
1
Clonality
Monoclonal
Conjugate
This Leptin Receptor antibody is un-conjugated
12
10
10
7
7
7
7
5
5
5
5
4
4
3
3
3
3
3
3
2
2
2
2
2
2
2
1
Application
Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS), Immunohistochemistry (IHC), Western Blotting (WB)
164
105
70
51
40
40
6
4
4
2
2
2
1
Supplier
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Catalog No. ABIN4899702
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN4899703 CyTOF FACS IHC WB Mouse IgG2b AA 22-839 Log in to see 52263 3

General

Antigen Leptin Receptor (LEPR) Antibodies
Epitope AA 22-839
(24), (18), (16), (15), (15), (15), (12), (6), (5), (5), (5), (5), (5), (4), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(221), (96), (64), (15), (7), (3), (3), (1), (1), (1), (1), (1), (1)
Host Mouse
(148), (67), (25), (16), (2), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Leptin Receptor antibody is un-conjugated
(12), (10), (10), (7), (7), (7), (7), (5), (5), (5), (5), (4), (4), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1)
Application Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS), Immunohistochemistry (IHC), Western Blotting (WB)
(164), (105), (70), (51), (40), (40), (6), (4), (4), (2), (2), (2), (1)
Pubmed 3 references available
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Product Details anti-Leptin Receptor Antibody

Target Details Leptin Receptor Application Details Handling References for anti-Leptin Receptor antibody (ABIN4899702) Images
Specificity Detects human Leptin R in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1 % cross-reactivity with recombinant mouse Leptin R is observed.
Purification Protein A or G purified from ascites
Sterility 0.2 μm filtered
Immunogen Mouse myeloma cell line NS0-derived recombinant human Leptin R, Phe22-Asp839 (predicted), Accession # P48357
Clone 52263
Isotype IgG2b

Target Details Leptin Receptor

Product Details anti-Leptin Receptor Antibody Application Details Handling References for anti-Leptin Receptor antibody (ABIN4899702) Images back to top
Antigen
Alternative Name Leptin R (LEPR Antibody Abstract)
Background Alternative Names: B219, CD295, CD295 antigen, DB, DKFZp686B1731, huB219, LEPR, LEP-R, leptin receptor, LeptinR, OB R, OB receptor, OB-R, OBRCD295
Gene ID 3953
UniProt P48357
Pathways JAK-STAT Signaling, AMPK Signaling, Feeding Behaviour

Application Details

Product Details anti-Leptin Receptor Antibody Target Details Leptin Receptor Handling References for anti-Leptin Receptor antibody (ABIN4899702) Images back to top
Application Notes Western Blot 1 μg/mL
Flow Cytometry 2.5 μg/10^6 cells
Immunohistochemistry 8-25 μg/mL
CyTOF-ready
Restrictions For Research Use only

Handling

Product Details anti-Leptin Receptor Antibody Target Details Leptin Receptor Application Details References for anti-Leptin Receptor antibody (ABIN4899702) Images back to top
Reconstitution Reconstitute at 0.5 mg/mL in sterile PBS.
Buffer Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Preservative Without preservative
Storage 4 °C,-20 °C,-80 °C
Storage Comment Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt,-20°C to -70°C as supplied. 1 month,2°C to 8°C under sterile conditions after reconstitution. 6 months,-20°C to -70°C under sterile conditions after reconstitution.
Expiry Date 12 months

References for anti-Leptin Receptor antibody (ABIN4899702)

Product Details anti-Leptin Receptor Antibody Target Details Leptin Receptor Application Details Handling Images back to top
Product cited in:

Liang, Gao, Liang, Ye, Peng, Zhang, Sharma, Su, Huang: "Normal leptin expression, lower adipogenic ability, decreased leptin receptor and hyposensitivity to Leptin in Adolescent Idiopathic Scoliosis." in: PLoS ONE, Vol. 7, Issue 5, pp. e36648, 2012 Method employed by authors: Western Blotting (WB) (Sample species: Human).

Hongo, Watanabe, Arita, Kanome, Kageyama, Shioda, Miyazaki: "Leptin modulates ACAT1 expression and cholesterol efflux from human macrophages." in: American journal of physiology. Endocrinology and metabolism, Vol. 297, Issue 2, pp. E474-82, 2009 Method employed by authors: Western Blotting (WB) (Sample species: Human).

Francois, Roper, Goodman, Pei, Ghumman, Mourad, de Perez, Perez-Perez, Tseng, Blaser: "The association of gastric leptin with oesophageal inflammation and metaplasia." in: Gut, Vol. 57, Issue 1, pp. 16-24, 2007 Method employed by authors: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Sample species: Human).

Images

Product Details anti-Leptin Receptor Antibody Target Details Leptin Receptor Application Details Handling References for anti-Leptin Receptor antibody (ABIN4899702) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-Leptin Receptor (LEPR) (AA 22-839) antibody (ABIN4899702) Detection of Leptin R in Human Monocytes by Flow Cytometry. Human whole blood monocyt...
Western Blotting (WB) image for anti-Leptin Receptor (LEPR) (AA 22-839) antibody (ABIN4899702) Detection of Recombinant Human Leptin R by Western Blot. Western blot shows 25 ng of ...