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HCP Protein

HCP Origin: E. coli Host: Escherichia coli (E. coli) Native SDS, 2D-PAGE, WB
Catalog No. ABIN1607597
  • Target See all HCP products
    HCP (Host Cell Proteins (HCP))
    Protein Type
    Native
    Origin
    E. coli
    Source
    Escherichia coli (E. coli)
    Application
    SDS-PAGE (SDS), Two Dimensional Polyacrylamide Gel Electrophoresis (2D-PAGE), Western Blotting (WB)
    Purpose
    E.coli Host Cell Protein Control Protein (HCP) (in 1x Loading Buffer)
    Specificity
    E. coli Host Cell Protein Control Protein was prepared by solubilizing mock induced E. coli (BL21) cells.
    Characteristics
    Host Cell Protein Lysate was prepared by solubilizing mock induced E.coli cells. Host Cell Proteins are process-related impurities derived from a host cell expression system that may be present in trace amounts in a final drug substance. HCP Antibodies are used to detect impurities in biologically-based therapeutics. Detecting impurities often unseen by 1-D electrophoresis, a 2D electrophoresis western blot analysis delivers more complete insight into the immunocoverage of protein components of the host cell lysate. Synonyms: E.coli Host Cell Proteins , HCP antigen, E.coli control antigen
  • Application Notes
    Western Blot Dilution: 1:500 - 1:1000
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    2.0 mg/mL
    Buffer
    1X SDS-PAGE Sample Buffer (62.5 mM Tris HCl, 2 % SDS, 10 % Glycerol and 0.005 % bromophenol blue, pH 6.8)
    Preservative
    Without preservative
    Storage
    -20 °C
    Storage Comment
    Store Host Cell Proteins control protein at -20 °C prior to opening. Aliquot contents and freeze at -20 °C or below for extended storage. Avoid cycles of freezing and thawing. Dilute only prior to immediate use.
    Expiry Date
    12 months
  • Target
    HCP (Host Cell Proteins (HCP))
    Alternative Name
    Host Cell Proteins (HCP Products)
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