SensoLyte® AFC Urokinase (uPA) Activity Assay Kit

Catalog No. ABIN1882570

Product Details

Target: PLAU (Plasminogen Activator, Urokinase (PLAU))

Detection Method: Fluorometric

Application: Activation (Act), Fluorescence Resonance Energy Transfer Microscopy (FRET)

Brand: SensoLyte®

Characteristics: The SensoLyte AFC Urokinase (uPA) Assay Kit is optimized for screening of enzyme inhibitors. This kit contains a fluorogenic substrate with a high reactivity and low background. uPA cleaves the substrate resulting in release of AFC (7-amino-4-trifluoromethylcoumarin) fluorophore. Fluorescence can be monitored at excitation /emission= 380/500 nm.

Application Details

Comment:

FRET-based Assay Kit

Restrictions: For Research Use only

Handling

Handling Advice: Aliquot as needed to avoid multiple freeze-thaw cycles. Protect Components A and B from light and moisture.

Storage: -80 °C

Storage Comment: Store all kit components, except Component D, at -20 °C. Store Component D at -80 °C. Components C can be stored at room temperature for convenience.

Target Details

Target: PLAU (Plasminogen Activator, Urokinase (PLAU))

Alternative Name: uPA (PLAU Products)

Synonyms: PLAU Kit, u-PA Kit, ATF Kit, BDPLT5 Kit, QPD Kit, UPA Kit, URK Kit, uPA Kit, UPAM Kit, plasminogen activator, urokinase Kit, urokinase-type plasminogen activator Kit, PLAU Kit, CpipJ_CPIJ002131 Kit, CpipJ_CPIJ006543 Kit, CpipJ_CPIJ013396 Kit, CpipJ_CPIJ013623 Kit, Plau Kit

Background: Urokinase-type plasminogen activator (uPA) is a serine protease that functions in the conversion of the zymogen plasminogen to the active, broad-spectrum serine protease plasmin. Plasmin, in turn, mediates the pericellular proteolysis of extracellular matrix components and activates other proteases such as matrix metalloproteinases and collagenases that lead to the further degradation and remodeling of the extracellular matrix. Besides the proteolytic function, upon binding to uPA receptor (uPAR), uPA is involved in initiating versatile intracellular signal pathways that regulate cell proliferation, adhesion, and migration through its interaction with various integrins and vitronectin. uPA has been implicated in a number of normal and pathological processes, and recognized as a prognostic marker for various cancers as well as a target for drug discovery.