IL-1 beta ELISA Kit

Catalog No. ABIN2042513

This Colorimetric ELISA kit is designed for the quantitative measurement of Human IL-1 beta.

Quick Overview for IL-1 beta ELISA Kit (ABIN2042513)

Target: IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 3.9-250 pg/mL

Application: ELISA

Product Details IL-1 beta ELISA Kit

Minimum Detection Limit: 3.9 pg/mL

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 0.15 pg/mL

Components: A: Microtiter Strip, 1x96-well Pre-coated with anti-IL-1 beta, Human
B: Standard, 1000pg/ml, 2x1vial Lyophilized recombinant IL-1 beta, Human
C: Pab (Biotin) GtxHu, 1x130 µL
: Avidin-Biotin-Peroxidase Complex (ABC), 1x130 µL (1:100 dilution)
: Sample diluent buffer, 1x30 mL
: Antibody diluent buffer, 1x10 mL
: ABC diluent buffer, 1x10 mL
: TMB color developing agent, 1x10 mL
: TMB stop solution, 2N H2SO4, 1x10 mL

Application Details

Plate: Pre-coated

Protocol: Sandwich ELISA quantitative detection kit for Human IL-1 beta in cell culture supernates, serum and plasma (heparin, EDTA, citrate).Interleukin-1b ( IL-1b) is a potent stimulator of bone resorption whose gene is mapped to 2q14, and has been implicated in the pathogenesis of high bone turnover and osteoporosis. IL-1b, a prominent microglia-derived cytokine, caused oligodendrocyte death in coculture with astrocytes and microglia, but not in pure culture of oligodendrocytes alone. It also can cause nuclear export of a specific NCOR corepressor complex, resulting in derepression of a specific subset of nuclear factor-kappa-B (NFKB)-regulated genes2. Furthermore, Microenvironmental IL-1b and, to a lesser extent, IL-1a are required for in vivo angiogenesis and invasiveness of different tumor cells. Additional, the cooperation of IL-1b and PDGFB induces contractile-to-synthetic phenotype modulation of human aortic smooth muscle cells in culture. Moreover, the association with disease may be explained by the biologic properties of IL-1b, which is an important proinflammatory cytokine and a powerful inhibitor of gastric acid secretion.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for IL-1 beta

Target: IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))

Alternative Name: Interleukin-1 beta

Gene ID: 3553

UniProt: P01584

Pathways: NF-kappaB Signaling, Interferon-gamma Pathway, TLR Signaling, Negative Regulation of Hormone Secretion, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Autophagy, Cancer Immune Checkpoints, Inflammasome