IL-1 beta ELISA Kit

Catalog No. ABIN2042514

This Mouse IL-1 beta ELISA Kit is a Colorimetric ELISA Kit designed to quantify Mouse IL-1 beta.

Quick Overview for IL-1 beta ELISA Kit (ABIN2042514)

Target: IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 12.5-800 pg/mL

Application: ELISA

Product Details IL-1 beta ELISA Kit

Minimum Detection Limit: 12.5 pg/mL

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 1 pg/mL

Components: 96-well plate pre-coated with anti-IL-1 beta, Mouse
Lyophilized recombinant IL-1 beta, Mouse standard, 10 ng/tube, 2Ea
Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
Sample diluent buffer, 30 mL
Antibody diluent buffer, 10 mL
ABC diluent buffer, 10 mL
TMB color developing agent, 10 mL
TMB stop solution, 10 mL

Application Details

Plate: Pre-coated

Protocol: Sandwich ELISA quantitative detection kit for Mouse IL-1 beta in cell culture supernates, serum and plasma (heparin, EDTA).Interleukin-1 ( IL-1 ) is a potent stimulator of bone resorption whose gene is mapped to 2q14, and has been implicated in the pathogenesis of high bone turnover and osteoporosis. IL-1 , a prominent microglia-derived cytokine, caused oligodendrocyte death in coculture with astrocytes and microglia, but not in pure culture of oligodendrocytes alone1. It also can cause nuclear export of a specific NCOR corepressor complex, resulting in derepression of a specific subset of nuclear factor-kappa-B (NFKB)-regulated genes2. Furthermore, Microenvironmental IL-1 and, to a lesser extent, IL-1 are required for in vivo angiogenesis and invasiveness of different tumor cells3. Additional, the cooperation of IL-1 and PDGFB induces contractile-to-synthetic phenotype modulation of human aortic smooth muscle cells in culture4. Moreover, the association with disease may be explained by the biologic properties of IL-1 , which is an important proinflammatory cytokine and a powerful inhibitor of gastric acid secretion.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for IL-1 beta

Target: IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))

Alternative Name: Interleukin-1 beta (IL-1 Beta)

Gene ID: 16176

UniProt: P10749

Pathways: NF-kappaB Signaling, Interferon-gamma Pathway, TLR Signaling, Negative Regulation of Hormone Secretion, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Autophagy, Cancer Immune Checkpoints, Inflammasome