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IL-1 beta ELISA Kit

Rat IL-1 beta ELISA Kit Colorimetric assay for quantification of Rat IL-1 beta.
Catalog No. ABIN2042515

Quick Overview for IL-1 beta ELISA Kit (ABIN2042515)

Target

See all IL-1 beta (IL1B) ELISA Kits
IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))

Reactivity

  • 23
  • 19
  • 14
  • 9
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  • 8
  • 5
  • 5
  • 4
  • 3
  • 3
  • 2
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  • 1
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  • 1
Rat

Detection Method

Colorimetric

Method Type

Sandwich ELISA

Detection Range

31.2-2000 pg/mL

Application

ELISA
  • Minimum Detection Limit

    31.2 pg/mL

    Cross-Reactivity (Details)

    There is no detectable cross-reactivity with other relevant proteins.

    Sensitivity

    < 1 pg/mL

    Components

    96-well plate pre-coated with anti-IL-1 beta, Rat
    Lyophilized recombinant IL-1 beta, Rat standard, 10 ng/tube, 2Ea
    Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
    Sample diluent buffer, 30 mL
    Antibody diluent buffer, 10 mL
    ABC diluent buffer, 10 mL
    TMB color developing agent, 10 mL
    TMB stop solution, 10 mL
  • Plate

    Pre-coated

    Protocol

    Sandwich ELISA quantitative detection kit for Rat IL-1 beta in cell culture supernates, serum and plasma (heparin, EDTA).The Interleukin 1 family is a group of 11 beta trefoil cytokines.1 Interleukin-1, produced mainly by blood monocytes, mediates the panoply of host reactions collectively known as acute phase response .2 Interleukin-1beta (IL-1beta) is a potent stimulator of bone resorption, and has been implicated in the pathogenesis of high bone turnover and osteoporosis.3 IL-1beta belongs to proinflammatory cytokines, promote cancer cell adhesion and liver metastases by up-regulating the expression of vascular cell adhesion molecule-1 (VCAM-1) on hepatic sinusoidal endothelium (HSE).4

    Restrictions

    For Research Use only
  • Storage

    4 °C/-20 °C
  • Target See all IL-1 beta (IL1B) ELISA Kits

    IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))

    Alternative Name

    Interleukin-1 beta (IL-1 Beta)

    Gene ID

    24494

    UniProt

    Q5BKB0

    Pathways

    NF-kappaB Signaling, Interferon-gamma Pathway, TLR Signaling, Negative Regulation of Hormone Secretion, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Autophagy, Cancer Immune Checkpoints, Inflammasome
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