HLA-DQB1 ELISA Kit

Catalog No. ABIN2043917

Product Details HLA-DQB1 ELISA Kit

Target: HLA-DQB1 (Major Histocompatibility Complex, Class II, DQ beta 1 (HLA-DQB1))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.15-10 ng/mL

Minimum Detection Limit: 0.15 ng/mL

Application: ELISA

Brand: BioAssay™

Sensitivity: < 0.065 ng/mL

Application Details

Plate: Pre-coated

Protocol: Major Histocompatibility Complex Class II DQ Beta 1 (MHCDQb1) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Major Histocompatibility Complex Class II DQ Beta 1 (MHCDQb1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Major Histocompatibility Complex Class II DQ Beta 1 (MHCDQb1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Major Histocompatibility Complex Class II DQ Beta 1 (MHCDQb1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Major Histocompatibility Complex Class II DQ Beta 1 (MHCDQb1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for HLA-DQB1

Target: HLA-DQB1 (Major Histocompatibility Complex, Class II, DQ beta 1 (HLA-DQB1))

Alternative Name: Major Histocompatibility Complex Class II DQ beta 1 (MHCDQb1) (HLA-DQB1 Products)

Synonyms: CELIAC1 ELISA Kit, HLA-DQB ELISA Kit, IDDM1 ELISA Kit, mhc-DRB ELISA Kit, DLA-DQB ELISA Kit, MAMU-DQB1 ELISA Kit, LOC100172120 ELISA Kit, DKFZp469C0237 ELISA Kit, major histocompatibility complex, class II, DQ beta 1 ELISA Kit, HLA-DQB1 ELISA Kit, PATR-DQB1 ELISA Kit, DLA-DQB1 ELISA Kit, MAMU-DQB1 ELISA Kit, LOC100172120 ELISA Kit

Pathways: TCR Signaling, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints