SIRT3 ELISA Kit
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- Target See all SIRT3 ELISA Kits
- SIRT3 (Sirtuin 3 (SIRT3))
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Reactivity
- Mouse
- Detection Method
- Colorimetric
- Method Type
- Sandwich ELISA
- Detection Range
- 0.15-10 ng/mL
- Minimum Detection Limit
- 0.15 ng/mL
- Application
- ELISA
- Brand
- BioAssay™
- Sensitivity
- < 0.055 ng/mL
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- Plate
- Pre-coated
- Protocol
- Sirtuin 3 (SIRT3) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sirtuin 3 (SIRT3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Sirtuin 3 (SIRT3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sirtuin 3 (SIRT3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Sirtuin 3 (SIRT3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- Restrictions
- For Research Use only
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- Storage
- 4 °C/-20 °C
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- Target See all SIRT3 ELISA Kits
- SIRT3 (Sirtuin 3 (SIRT3))
- Abstract
- SIRT3 Products
- Synonyms
- zgc:158774 ELISA Kit, sirtuin ELISA Kit, SIR2L3 ELISA Kit, 2310003L23Rik ELISA Kit, AI848213 ELISA Kit, Sir2l3 ELISA Kit, sirtuin 3 ELISA Kit, sirt3 ELISA Kit, SIRT3 ELISA Kit, Sirt3 ELISA Kit
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