Insulin ELISA Kit

Catalog No. ABIN2641859

Product Details Insulin ELISA Kit

Target: Insulin (INS)

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 15.6-1000 nlU/mL

Minimum Detection Limit: 15.6 nlU/mL

Application: ELISA

Purpose: For the quantitative determination of rat insulin (INS) concentrations in serum, plasma, cell culture supernates.

Sample Type: Serum, Plasma, Cell Culture Supernatant

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of rat INS.

Cross-Reactivity (Details): Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between the target antigen and all analogues for other species. Therefore, cross reaction may still exist.

Sensitivity: 3.9 nlU/mL

Components:

• Assay plate (12 × 8 coated Microwells)
• Standard (freeze dried)
• Biotin-antibody (100 × concentrate)
• HRP-avidin (100 × concentrate)
• Biotin-antibody Diluent
• HRP-avidin Diluent
• Sample Diluent
• Wash Buffer (25 × concentrate)
• TMB Substrate
• Stop Solution
• Adhesive Strip (for 96 wells)
• Instruction manual

Application Details

Application Notes:

• The supplier is only responsible for the kit itself, but not for the samples consumed during the assay. The user should calculate the possible amount of the samples used in the whole test. Please reserve sufficient samples in advance.
• Samples to be used within 5 days may be stored at 2-8°C, otherwise samples must be stored at -20°C (≤ 1 month) or -80°C (≤ 2 months) to avoid loss of bioactivity and contamination.
• Grossly hemolyzed samples are not suitable for use in this assay.
• If the samples are not indicated in the manual, a preliminary experiment to determine the validity of the kit is necessary.
• Please predict the concentration before assaying. If values for these are not within the range of the standard curve, users must determine the optimal sample dilutions for their particular experiments.
• Tissue or cell extraction samples prepared by chemical lysis buffer may cause unexpected ELISA results due to the impacts of certain chemicals.
• Owing to the possibility of mismatching between antigens from another resource and antibodies used in this supplier's kits (e.g., antibody targets conformational epitope rather than linear epitope), some native or recombinant proteins from other manufacturers may not be recognized by this supplier's products.
• Influenced by factors including cell viability, cell number and cell sampling time, samples from cell culture supernatant may not be recognized by the kit.
• Fresh samples without long time storage are recommended for the test. Otherwise, protein degradation and denaturalization may occur in those samples and finally lead to wrong results.

Comment:

Detection wavelength: 450 nm

Information on standard material:
Depending on the antigen to be detected, standards can be either native or recombinant protein. The recombinant proteins are being expressed in CHO cells in most cases. Please inquire for more information. The formulation of auxiliary material in the standard is considered proprietary information, however it does not contain any poisonous substance. Proclin 300 (1:3000) is used as preservative.

Information on reagents:
In most cases the stop solution provided is 1 N H2SO4. The formulation of wash solution is proprietary information. None of the components contain (sodium) azide, thimerosal, 2-mercaptoethanol (2-ME) or any other poisonous materials. For the sandwich method kits, the sample diluent, antibody diluent, enzyme diluent and standard all contain BSA.

Information on antibodies:
The antibodies provided in different kits vary in regards to clonality and host. Some antibodies are affinity purified, some are Protein A

Sample Volume: 100 μL

Assay Time: 1 - 4.5 h

Plate: Pre-coated

Protocol: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for INS has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any INS present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for INS is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of INS bound in the initial step. The color development is stopped and the intensity of the color is measured.

Assay Precision: Intra-assay precision (precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess precision.
Inter-assay precision (precision between assays): Three samples of known concentration were tested in twenty assays to assess precision.

• Intra-assay: CV% less than 8%
• Inter-assay: CV% less than 10%

Restrictions: For Research Use only

Handling

Precaution of Use: The Stop Solution provided with this kit is an acid solution. Wear eye, hand, face and clothing protection when using this material.

Handling Advice:

• The kit should not be used beyond the expiration date on the kit label.
• Do not mix or substitute reagents with those from other lots or sources.
• If samples generate values higher than the highest standard, dilute the samples with Sample Diluent and repeat the assay.
• Any variation in Sample Diluent, operator, pipetting technique, washing technique, incubation time/temperature and kit age can cause variation in binding.
• This assay is designed to eliminate interference by soluble receptors, binding proteins and other factors present in biological samples. Until all factors have been tested in the Immunoassay, the possibility of interference cannot be excluded.

Storage: 4 °C/-20 °C

Storage Comment: For unopened kit: All the reagents should be kept according to the labels on vials.

Expiry Date: 6 months

References for Insulin ELISA Kit (ABIN2641859)

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El-Shemi, Kensara, Alsaegh, Mukhtar et al.: "Pharmacotherapy with Thymoquinone Improved Pancreatic β-Cell Integrity and Functional Activity, Enhanced Islets Revascularization, and Alleviated Metabolic and Hepato-Renal Disturbances in ..." in: Pharmacology, Vol. 101, Issue 1-2, pp. 9-21, (2018) (PubMed).

Elseweidy, Amin, Atteia, Aly: "Nigella sativa Oil and Chromium Picolinate Ameliorate Fructose-Induced Hyperinsulinemia by Enhancing Insulin Signaling and Suppressing Insulin-Degrading Enzyme in Male Rats." in: Biological trace element research, Vol. 184, Issue 1, pp. 119-126, (2018) (PubMed).

Hou, Hu, Yang, Chen: "Antihypertensive effects of Tartary buckwheat flavonoids by improvement of vascular insulin sensitivity in spontaneously hypertensive rats." in: Food & function, Vol. 8, Issue 11, pp. 4217-4228, (2018) (PubMed).

Zhao, Zhang, Ma, Tian, Shen, Zhou: "A combination of quercetin and resveratrol reduces obesity in high-fat diet-fed rats by modulation of gut microbiota." in: Food & function, Vol. 8, Issue 12, pp. 4644-4656, (2018) (PubMed).

Zheng, Niu: "Leptin-induced basal Akt phosphorylation and its implication in exercise-mediated improvement of insulin sensitivity." in: Biochemical and biophysical research communications, Vol. 496, Issue 1, pp. 37-43, (2018) (PubMed).

Li, Ni, Zhao, Liu, Lai, Di, Xie, Song, Wang, Zhang, Liu: "Melatonin attenuates smoking-induced hyperglycemia via preserving insulin secretion and hepatic glycogen synthesis in rats." in: Journal of pineal research, Vol. 64, Issue 4, pp. e12475, (2018) (PubMed).

Long, Zhang, Sun, Liu, Liao, Wu, Wang, Hai: "Evolution of metabolic disorder in rats fed high sucrose or high fat diet: Focus on redox state and mitochondrial function." in: General and comparative endocrinology, Vol. 242, pp. 92-100, (2017) (PubMed).

Zhang, Yu, Xu, Wang, Ji, Gu, Yang, Zhu, Dong, Wang: "High-fat diet aggravates 2,2',4,4'-tetrabromodiphenyl ether-inhibited testosterone production via DAX-1 in Leydig cells in rats." in: Toxicology and applied pharmacology, Vol. 323, pp. 1-8, (2017) (PubMed).

Shen, Yang, Yan, Zheng, Liang, Cai, Liao: "Fetuin A promotes lipotoxicity in ? cells through the TLR4 signaling pathway and the role of pioglitazone in anti-lipotoxicity." in: Molecular and cellular endocrinology, Vol. 412, pp. 1-11, (2015) (PubMed).

Jia, Ma, Liu, Zhou, He, Xu, Ren, Xu, Tian: "Metformin prevents DMH-induced colorectal cancer in diabetic rats by reversing the warburg effect." in: Cancer medicine, (2015) (PubMed).

Wu, Sun, Yin, Xu, Wang, Lin, Lin, Lin: "Different effect of handle region peptide on β-cell function in different sexes of rats neonatally treated with sodium L-glutamate." in: Medical science monitor basic research, Vol. 21, pp. 33-40, (2015) (PubMed).

Secher, Østergaard, Iversen, Lambertsen, Clausen, Tønnesen, Granfeldt: "Preserved Cerebral Microcirculation After Cardiac Arrest in a Rat Model." in: Microcirculation (New York, N.Y. : 1994), Vol. 22, Issue 6, pp. 464-74, (2015) (PubMed).

Yin, Lin, Xu, Sun, Lin, Lin: "Handle Region Peptide Ameliorating Insulin Resistance but Not β Cell Functions in Male Rats Neonatally Treated with Sodium L-Glutamate." in: International journal of endocrinology, Vol. 2013, pp. 493828, (2014) (PubMed).

Perimenis, Bouckenooghe, Delplanque, Moitrot, Eury, Lobbens, Gosset, Devisme, Duvillie, Abderrahmani, Storme, Fontaine, Froguel, Vambergue: "Placental antiangiogenic prolactin fragments are increased in human and rat maternal diabetes." in: Biochimica et biophysica acta, Vol. 1842, Issue 9, pp. 1783-93, (2014) (PubMed).

Zeng, Wang, Li, Shen, Wang, Yu, Wang: "AKAP150 mobilizes cPKC-dependent cardiac glucotoxicity." in: American journal of physiology. Endocrinology and metabolism, Vol. 307, Issue 4, pp. E384-97, (2014) (PubMed).

Lu, Zhang, Zheng, Jiang, Chen: "Branched-chain amino acids supplementation protects streptozotocin-induced insulin secretion and the correlated mechanism." in: BioFactors (Oxford, England), (2014) (PubMed).

Wang, Luo, Wang, Li, Wang, Sun, Zhang, Su, Ma, Zeng, Wang, Ren, Cao: "Glucagon-like peptide-1 protects against cardiac microvascular injury in diabetes via a cAMP/PKA/Rho-dependent mechanism." in: Diabetes, Vol. 62, Issue 5, pp. 1697-708, (2013) (PubMed).

Zhang, Sun, Xiao, Zhou, Wang, Gu, Qiu, Zhang, Xu, Zhen, Wang, Wang: "Mechanism of BDE209-induced impaired glucose homeostasis based on gene microarray analysis of adult rat liver." in: Archives of toxicology, Vol. 87, Issue 8, pp. 1557-67, (2013) (PubMed).

Wang, Liu, Zhang, Zhang, Liao, Wang, Li, Qin, Hai: "Oleanolic acid improves hepatic insulin resistance via antioxidant, hypolipidemic and anti-inflammatory effects." in: Molecular and cellular endocrinology, Vol. 376, Issue 1-2, pp. 70-80, (2013) (PubMed).

Target Details for Insulin

Target: Insulin (INS)

Abstract: INS Products

Synonyms: IDDM2 ELISA Kit, ILPR ELISA Kit, IRDN ELISA Kit, MODY10 ELISA Kit, ins1 ELISA Kit, xins ELISA Kit, ins1-a ELISA Kit, Insulin ELISA Kit, AA986540 ELISA Kit, Ins-2 ELISA Kit, InsII ELISA Kit, Mody ELISA Kit, Mody4 ELISA Kit, proinsulin ELISA Kit, zgc:109842 ELISA Kit, igf2-A ELISA Kit, ins ELISA Kit, ins-a ELISA Kit, ins-b ELISA Kit, insulin ELISA Kit, insulin precursor ELISA Kit, insulin II ELISA Kit, preproinsulin ELISA Kit, insulin L homeolog ELISA Kit, insulin S homeolog ELISA Kit, INS ELISA Kit, INS-IGF2 ELISA Kit, ins ELISA Kit, Ins ELISA Kit, PIN ELISA Kit, Ins2 ELISA Kit, ins.L ELISA Kit, ins.S ELISA Kit

Pathways: NF-kappaB Signaling, RTK Signaling, Positive Regulation of Peptide Hormone Secretion, Peptide Hormone Metabolism, Hormone Activity, Carbohydrate Homeostasis, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Autophagy, Negative Regulation of intrinsic apoptotic Signaling, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation