IL-8 ELISA Kit
-
- Target See all IL-8 (IL8) ELISA Kits
- IL-8 (IL8) (Interleukin 8 (IL8))
-
Reactivity
- Human
- Detection Method
- Colorimetric
- Application
- ELISA
- Purpose
- Human Interleukin 8 (IL-8) ELISA Assay Kit (enzyme-linked immunoassay kit) is intended for the quantitative determination of human Interleukin 8 (IL-8) concentrations in cell culture supernates, serum, and plasma.
- Sample Type
- Serum, Plasma, Cell Culture Cells
- Analytical Method
- Quantitative
- Sensitivity
- 4 pg/ml
- Characteristics
- Human Interleukin 8 (IL-8) ELISA Assay Kit is for research use only and not to be used in diagnostic procedures.
- Featured
- Discover our best selling IL8 ELISA Kit
- Top Product
- Discover our top product IL8 ELISA Kit
-
-
- Application Notes
- Optimal working dilution should be determined by the investigator.
- Sample Volume
- 100 μL
- Assay Time
- 3.5 h
- Plate
- Pre-coated
- Protocol
- This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for IL-8 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-8 present is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and then a detection antibody specific for IL-8 is added to the wells and binds to the combination of capture antibody-IL-8 in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A colored product is formed in proportion to the amount of IL-8 present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven IL-8 standard dilutions and IL-8 sample concentration determined.
- Restrictions
- For Research Use only
-
- Storage
- 4 °C
-
- Target See all IL-8 (IL8) ELISA Kits
- IL-8 (IL8) (Interleukin 8 (IL8))
- Alternative Name
- IL-8 (IL8 Products)
- Synonyms
- CXCL8 ELISA Kit, GCP-1 ELISA Kit, GCP1 ELISA Kit, LECT ELISA Kit, LUCT ELISA Kit, LYNAP ELISA Kit, MDNCF ELISA Kit, MONAP ELISA Kit, NAF ELISA Kit, NAP-1 ELISA Kit, NAP1 ELISA Kit, IL-8 ELISA Kit, AMCF-I ELISA Kit, il8 ELISA Kit, IL8 ELISA Kit, si:dkey-151b16.2 ELISA Kit, cxcli2 ELISA Kit, cxcl8 ELISA Kit, gcp-1 ELISA Kit, il-8 ELISA Kit, lynap ELISA Kit, mdncf ELISA Kit, monap ELISA Kit, nap-1 ELISA Kit, C-X-C motif chemokine ligand 8 ELISA Kit, interleukin 8 ELISA Kit, chemokine (C-X-C motif) ligand 8a ELISA Kit, chemokine (C-X-C motif) ligand 1-like ELISA Kit, chemokine (C-X-C motif) ligand 8 L homeolog ELISA Kit, CXCL8 ELISA Kit, il8 ELISA Kit, cxcl8a ELISA Kit, IL8 ELISA Kit, Cxcl8 ELISA Kit, LOC422654 ELISA Kit, cxcl8.L ELISA Kit
- Pathways
- TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of G-Protein Coupled Receptor Protein Signaling, ER-Nucleus Signaling, Hepatitis C, Autophagy
-