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PEDF ELISA Kit
SERPINF1
Reactivity: Human, Mouse
Colorimetric
Sandwich ELISA
0.6-160 μg/mL
Cell Culture Supernatant, Plasma, Serum
Product Details PEDF ELISA Kit
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Target
See all PEDF (SERPINF1) ELISA Kits
PEDF (SERPINF1)
(serpin Peptidase Inhibitor, Clade F (Alpha-2 Antiplasmin, Pigment Epithelium Derived Factor), Member 1 (SERPINF1))
Reactivity
All reactivities for PEDF elisa kits
Human, Mouse
Detection Method
Colorimetric
Method Type
Sandwich ELISA
Detection Range
0.6-160 μg/mL
Minimum Detection Limit
0.6 μg/mL
Application
ELISA
Purpose
Human SERPINF1 (PEDF) ELISA Kit for cell culture supernatants, plasma, and serum samples.
Sample Type
Cell Culture Supernatant, Plasma, Serum
Analytical Method
Quantitative
Specificity
This ELISA kit detects human Serpin F1. Approximately 40 % cross-reactivity with recombinant mouse Serpin F1 is observed in this antibody pair.
Sensitivity
0.6 μg/mL
Characteristics
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Components
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Material not included
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Application Details
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Application Notes
Recommended Dilution for serum and plasma samples2 fold
Sample Volume
100 μL
Plate
Pre-coated
Protocol
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Restrictions
For Research Use only
Handling
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Storage
-20 °C
Storage Comment
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Expiry Date
6 months
Target Details for PEDF
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Target
See all PEDF (SERPINF1) ELISA Kits
PEDF (SERPINF1)
(serpin Peptidase Inhibitor, Clade F (Alpha-2 Antiplasmin, Pigment Epithelium Derived Factor), Member 1 (SERPINF1))
Alternative Name
Serpin F1 (SERPINF1 Products )
Synonyms
EPC-1 ELISA Kit, OI12 ELISA Kit, OI6 ELISA Kit, PEDF ELISA Kit, MGC82984 ELISA Kit, SERPINF1 ELISA Kit, AI195227 ELISA Kit, Pedf ELISA Kit, Pedfl ELISA Kit, Sdf3 ELISA Kit, Dmrs91 ELISA Kit, zgc:92072 ELISA Kit, serpin family F member 1 ELISA Kit, serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1 ELISA Kit, serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1 L homeolog ELISA Kit, serine (or cysteine) peptidase inhibitor, clade F, member 1 ELISA Kit, SERPINF1 ELISA Kit, serpinf1 ELISA Kit, serpinf1.L ELISA Kit, Serpinf1 ELISA Kit
Background
Gene Names: SERPINF1 PEDF PIG35 Protein names: Pigment epithelium-derived factor (PEDF) (Cell proliferation-inducing gene 35 protein) (EPC-1) (Serpin F1)
Gene ID
5176
UniProt
P36955
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