TNFSF4
(Tumor Necrosis Factor (Ligand) Superfamily, Member 4 (TNFSF4))
Reactivity
Mouse
Detection Method
Colorimetric
Method Type
Sandwich ELISA
Detection Range
30-8000 pg/mL
Minimum Detection Limit
30 pg/mL
Application
ELISA
Purpose
Mouse OX40 Ligand (TNFSF4) ELISA Kit for cell culture supernatants, plasma, and serum samples.
Sample Type
Cell Culture Supernatant, Plasma, Serum
Analytical Method
Quantitative
Specificity
This ELISA antibody pair detects mouse TNFSF4/OX40 Ligand and on cross reactivity with rmBAFF, rmEDA, rmGITR Ligand, rmFas Ligand, rmLIGHT, rmTWEAK, rhAPRIL, or rhTNFSF4.
Sensitivity
30 pg/mL
Characteristics
Strip plates and additional reagents allow for use in multiple experiments
Quantitative protein detection
Establishes normal range
The best products for confirmation of antibody array data
Components
Pre-Coated 96-well Strip Microplate
Wash Buffer
Stop Solution
Assay Diluent(s)
Lyophilized Standard
Biotinylated Detection Antibody
Streptavidin-Conjugated HRP
TMB One-Step Substrate
Material not included
Distilled or deionized water
Precision pipettes to deliver 2 μL to 1 μL volumes
Adjustable 1-25 μL pipettes for reagent preparation
100 μL and 1 liter graduated cylinders
Tubes to prepare standard and sample dilutions
Absorbent paper
Microplate reader capable of measuring absorbance at 450nm
Log-log graph paper or computer and software for ELISA data analysis
Recommended Dilution for serum and plasma samples2 fold
Sample Volume
100 μL
Plate
Pre-coated
Protocol
Prepare all reagents, samples and standards as instructed in the manual.
Add 100 μL of standard or sample to each well.
Incubate 2.5 h at RT or O/N at 4 °C.
Add 100 μL of prepared biotin antibody to each well.
Incubate 1 h at RT.
Add 100 μL of prepared Streptavidin solution to each well.
Incubate 45 min at RT.
Add 100 μL of TMB One-Step Substrate Reagent to each well.
Incubate 30 min at RT.
Add 50 μL of Stop Solution to each well.
Read at 450 nm immediately.
Restrictions
For Research Use only
Storage
-20 °C
Storage Comment
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.