p53 ELISA Kit

Catalog No. ABIN2748489

Product Details p53 ELISA Kit

Target: p53 (TP53) (Tumor Protein P53 (TP53))

Binding Specificity: pSer15

Reactivity: Human, Rat, Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Human/Mouse/Rat Phospho-P53 (S15) ELISA Kit. This assay semi-quantitatively measures phophorylated P53 (Ser15) in lysate samples.

Sample Type: Cell Lysate, Tissue Lysate

Analytical Method: Semi-Quantitative

Specificity: The antibody pair provided in this kit recognizes human Phospho-P53 (pSer15).

Characteristics:

• Rapidly measure phosphorylated protein in lysates
• Screen numerous different cell lysates without performing a Western Blot analysis
• Minimal hands-on time, convenient, and non-radioactive material

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Anti-Phospho Antibody
• HRP-Conjugated Secondary Antibody
• Assay Diluent
• TMB One-Step Substrate
• Stop Solution
• Lysis Buffer
• Positive Control Sample

Material not included:

• Distilled or deionized water
• 100 mL and 1 liter graduated cylinders
• Tubes to prepare sample dilutions
• Protease and Phosphatase inhibitors
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• Benchtop rocker or shaker
• Microplate reader capable of measuring absorbance at 450 nm

Application Details

Sample Volume: 100 μL

Plate: Pre-coated

Protocol:

1. Prepare all reagents and samples as instructed in the manual.
2. Add 100 μL of sample or positive control to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared primary antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
7. Incubate 1 h at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Assay Procedure:

Prepare all reagents and samples as instructed in the manual.
Add 100 μL of sample or positive control to each well.
Incubate 2.5 h at RT or O/N at 4 °C.
Add 100 μL of prepared primary antibody to each well.
Incubate 1 h at RT.
Add 100 μL of prepared 1X HRP-Streptavidin to each well.
Incubate 1 h at RT.
Add 100 μL of TMB One-Step Substrate Reagent to each well.
Incubate 30 min at RT.
Add 50 μL of Stop Solution to each well.
Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.

Expiry Date: 6 months

Target Details for p53

Target: p53 (TP53) (Tumor Protein P53 (TP53))

Alternative Name: P53 (TP53 Products)

Synonyms: BCC7 ELISA Kit, LFS1 ELISA Kit, P53 ELISA Kit, TRP53 ELISA Kit, p53 ELISA Kit, Trp53 ELISA Kit, brp53 ELISA Kit, drp53 ELISA Kit, etID22686.5 ELISA Kit, fb40d06 ELISA Kit, wu:fb40d06 ELISA Kit, zgc:111919 ELISA Kit, Tp53 ELISA Kit, bbl ELISA Kit, bfy ELISA Kit, bhy ELISA Kit, p44 ELISA Kit, tp53 ELISA Kit, Xp53 ELISA Kit, TP53 ELISA Kit, tumor protein p53 ELISA Kit, cellular tumor antigen p53 ELISA Kit, transformation related protein 53 ELISA Kit, tumor protein p53 L homeolog ELISA Kit, TP53 ELISA Kit, CpipJ_CPIJ002758 ELISA Kit, Tp53 ELISA Kit, tp53 ELISA Kit, Trp53 ELISA Kit, tp53.L ELISA Kit

Background: P53 (Tumor protein p53, cellular tumor antigen p53, phosphoprotein p53, tumor suppressor p53) phosphorylated at Serine-15

Gene ID: 7157

UniProt: P04637

Pathways: p53 Signaling, MAPK Signaling, PI3K-Akt Signaling, Apoptosis, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagy, Warburg Effect