IGF1 ELISA Kit

Catalog No. ABIN4881446

Product Details IGF1 ELISA Kit

Target: IGF1 (Insulin-Like Growth Factor 1 (IGF1))

Reactivity: Human, Cow

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 1.23-300 ng/mL

Minimum Detection Limit: 1.23 ng/mL

Application: ELISA

Purpose: Bovine IGF-1 ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.

Sample Type: Plasma, Cell Culture Supernatant, Serum

Analytical Method: Quantitative

Specificity: This ELISA antibody pair recognizes Bovine IGF-1.

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Recommended Dilution for serum and plasma samples2 fold

Sample Volume: 100 μL

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

Target Details for IGF1

Target: IGF1 (Insulin-Like Growth Factor 1 (IGF1))

Alternative Name: IGF-1 (IGF1 Products)

Synonyms: IGF-I ELISA Kit, IGF1A ELISA Kit, IGFI ELISA Kit, C730016P09Rik ELISA Kit, Igf-1 ELISA Kit, Igf-I ELISA Kit, Npt2B ELISA Kit, IGF-1 ELISA Kit, IGFIA ELISA Kit, IGF-IB ELISA Kit, igf1 ELISA Kit, IGF-1L ELISA Kit, IGF-1a ELISA Kit, IGF1 ELISA Kit, igf-1 ELISA Kit, igf1-A ELISA Kit, igf1.S ELISA Kit, xigf1 ELISA Kit, insulin like growth factor 1 ELISA Kit, insulin-like growth factor 1 ELISA Kit, insulin-like growth factor I ELISA Kit, insulin like growth factor 1 L homeolog ELISA Kit, IGF1 ELISA Kit, Igf1 ELISA Kit, LOC100136741 ELISA Kit, igf1 ELISA Kit, LOC678666 ELISA Kit, igf1.L ELISA Kit, LOC104911603 ELISA Kit

Gene ID: 281239

UniProt: P07455

Pathways: RTK Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Peptide Hormone Metabolism, Hormone Activity, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Stem Cell Maintenance, Glycosaminoglycan Metabolic Process, Regulation of Carbohydrate Metabolic Process, Autophagy, Smooth Muscle Cell Migration, Activated T Cell Proliferation, Positive Regulation of fat Cell Differentiation