p53 ELISA Kit
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- Target See all p53 (TP53) ELISA Kits
- p53 (TP53) (Tumor Protein P53 (TP53))
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Reactivity
- Human, Rat, Mouse
- Detection Method
- Colorimetric
- Method Type
- Sandwich ELISA
- Detection Range
- 0.328-80 ng/mL
- Minimum Detection Limit
- 0.328 ng/mL
- Application
- ELISA
- Purpose
- Rat p53 ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.
- Sample Type
- Plasma, Cell Culture Supernatant, Serum
- Analytical Method
- Quantitative
- Specificity
- This ELISA antibody pair recognizes Rat p53.
- Sensitivity
- 0.32 ng/mL
- Characteristics
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- Components
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- Material not included
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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- Discover our top product TP53 ELISA Kit
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- Application Notes
- Recommended Dilution for serum and plasma samples2 fold
- Sample Volume
- 100 μL
- Plate
- Pre-coated
- Protocol
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- Restrictions
- For Research Use only
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- Storage
- -20 °C
- Storage Comment
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- Expiry Date
- 6 months
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- Target See all p53 (TP53) ELISA Kits
- p53 (TP53) (Tumor Protein P53 (TP53))
- Alternative Name
- p53 (TP53 Products)
- Synonyms
- BCC7 ELISA Kit, LFS1 ELISA Kit, P53 ELISA Kit, TRP53 ELISA Kit, p53 ELISA Kit, Trp53 ELISA Kit, brp53 ELISA Kit, drp53 ELISA Kit, etID22686.5 ELISA Kit, fb40d06 ELISA Kit, wu:fb40d06 ELISA Kit, zgc:111919 ELISA Kit, Tp53 ELISA Kit, bbl ELISA Kit, bfy ELISA Kit, bhy ELISA Kit, p44 ELISA Kit, tp53 ELISA Kit, Xp53 ELISA Kit, TP53 ELISA Kit, tumor protein p53 ELISA Kit, cellular tumor antigen p53 ELISA Kit, transformation related protein 53 ELISA Kit, tumor protein p53 L homeolog ELISA Kit, TP53 ELISA Kit, CpipJ_CPIJ002758 ELISA Kit, Tp53 ELISA Kit, tp53 ELISA Kit, Trp53 ELISA Kit, tp53.L ELISA Kit
- Gene ID
- 24842
- UniProt
- P10361
- Pathways
- p53 Signaling, MAPK Signaling, PI3K-Akt Signaling, Apoptosis, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagy, Warburg Effect
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