EPO ELISA Kit

Catalog No. ABIN5026954

Product Details EPO ELISA Kit

Target: EPO (Erythropoietin (EPO))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 31.25-2000 pg/mL

Minimum Detection Limit: 31.25 pg/mL

Application: ELISA

Purpose: This immunoassay kit allows for the in vitro quantitative determination of Human EPO concentrations in serum, plasma and other biological fluids.

Sample Type: Plasma, Serum

Analytical Method: Quantitative

Sensitivity: < 18.75 pg/mL

Components: plate, standard, diluent, antibodies

Material not included: pipettes, tubes, reader, incubator

Application Details

Sample Volume: 100 μL

Assay Time: 3 - 4 h

Plate: Pre-coated

Protocol: This assay employs an antibody specific for Anti-human EPO coated on a 96-well plate. Standards and samples are pipetted into the wells and EPO present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human EPO antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of EPO bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Restrictions: For Research Use only

Handling

Precaution of Use: protect your eyes

Storage: 4 °C

Target Details for EPO

Target: EPO (Erythropoietin (EPO))

Alternative Name: EPO (Erythropoietin) (EPO Products)

Synonyms: EPO ELISA Kit, EP ELISA Kit, MVCD2 ELISA Kit, erythropoietin ELISA Kit, erythropoietin S homeolog ELISA Kit, erythropoietin a ELISA Kit, EPO ELISA Kit, epo ELISA Kit, epo.S ELISA Kit, Epo ELISA Kit, epoa ELISA Kit

Target Type: Hormone

Pathways: JAK-STAT Signaling, Hormone Activity, Negative Regulation of intrinsic apoptotic Signaling, Negative Regulation of Transporter Activity