SLC30A8 ELISA Kit

Catalog No. ABIN5068025

Product Details SLC30A8 ELISA Kit

Target: SLC30A8 (Solute Carrier Family 30 (Zinc Transporter), Member 8 (SLC30A8))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: The SLC30A8 (Zinc transporter 8) ELISA Kit (Human) is a quantitative sandwich assay for the detection of SLC30A8 in human serum, plasma, tissue homogenates and other biological fluids.

Sample Type: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Sensitivity: > 0.188 ng/mL

Characteristics: SLC30A8 (Zinc Transporter 8) ELISA Kit (Human)

Components:

• *358913A: Microtiter Strips, 8x12 wells.
• *358913B: Standard, 2 vials
• 358913C: Sample/Standard Dilution Buffer, 1x20ml
• 358913D: Antibody-Biotin (Concentrated), 1x120ul
• 358913E: Antibody Dilution Buffer, 1x10ml
• 358913F: Streptavidin-HRP Conjugate (SABC), 1x120ul
• 358913G: SABC Dilution Buffer, 1x10ml
• 358913H: TMB Substrate, 1x10ml
• 358913J: Stop Solution, 1x10ml
• 358913K: Wash Buffer (25X), 1x30ml

Application Details

Plate: Pre-coated

Protocol: Principle:

• The microtiter plate provided in this kit has been pre-coated with an antibody specific to SLC30A8 . Standards and samples are added to the appropriate microtiter plate wells followed by a biotin-conjugated antibody specific to SLC30A8. Streptavidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain SLC30A8, Biotin-conjugated antibody and enzyme-conjugated Streptavidin complex will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of SLC30A8 in the sample is then determined by comparing the O.D. of the sample to the standard curve.

Assay Precision: Precision:

• Intra-Assay CV: <8%
• Inter-Assay CV: <10%

Restrictions: For Research Use only

Handling

Storage: 4 °C,-20 °C

Storage Comment: 4°C/-20°C

Target Details for SLC30A8

Target: SLC30A8 (Solute Carrier Family 30 (Zinc Transporter), Member 8 (SLC30A8))

Alternative Name: SLC30A8 (Zinc Transporter 8) (SLC30A8 Products)

Synonyms: C820002P14Rik ELISA Kit, ZnT-8 ELISA Kit, ZnT8 ELISA Kit, slc30a3 ELISA Kit, znt8 ELISA Kit, ZNT8 ELISA Kit, E130106K10Rik ELISA Kit, Gm212 ELISA Kit, wu:fe49g03 ELISA Kit, RGD1305098 ELISA Kit, solute carrier family 30 member 8 ELISA Kit, solute carrier family 30 (zinc transporter), member 8 ELISA Kit, solute carrier family 30 (zinc transporter), member 8 L homeolog ELISA Kit, solute carrier family 30, member 10 ELISA Kit, SLC30A8 ELISA Kit, Slc30a8 ELISA Kit, slc30a8.L ELISA Kit, slc30a8 ELISA Kit, Slc30a10 ELISA Kit

Pathways: Positive Regulation of Peptide Hormone Secretion, Hormone Transport, Carbohydrate Homeostasis, Transition Metal Ion Homeostasis