IL-1 beta ELISA Kit

Catalog No. ABIN5520914

This Mouse IL-1 beta ELISA Kit is a Colorimetric ELISA Kit designed to quantify Mouse IL-1 beta. This ELISA Kit has been cited in1 publication.

Quick Overview for IL-1 beta ELISA Kit (ABIN5520914)

Target: IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 12.5 pg/mL - 800 pg/mL

Application: ELISA

Sample Type: Plasma, Serum, Tissue Homogenate

Product Details IL-1 beta ELISA Kit

Minimum Detection Limit: 12.5 pg/mL

Purpose: For quantitative detection of IL-1β in serum, plasma, tissue homogenates.

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of IL-1β. No significant cross-reactivity or interference between IL-1β and analogues was observed. Note: Limited by current skills and knowledge, it is difficult for us to complete the cross-reactivity detection between IL-1β and all the analogues, therefore, cross reaction may still exist.

Sensitivity: 7.5 pg/mL

Components: - ELISA Microplate
- Lyophilized Standard
- Sample / Standard Dilution Buffer
- Biotin-labeled Antibody (Concentrated)
- Antibody Dilution Buffer
- HRP-Streptavidin Conjugate(SABC)
- SABC Dilution Buffer
- TMB Substrate
- Stop Solution
- Wash Buffer (25X)
- Plate Sealer
- Product Description

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Sandwich ELISA, Double Antibody

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment:

1. For unopened kit: All the reagents should be kept according to the labels on vials. The Reference Standard and the 96-well stripe plate should be stored at -20 °C upon receipt while the other reagents should be stored at 4 °C.
2. For used kit: When the kit is used, the remaining reagents need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and zip-seal the foil pouch.

Expiry Date: 6 months

References for IL-1 beta ELISA Kit (ABIN5520914)

Maresch, Stute, Ludlow, Hammes, de Kretser, Hedger, Linn: "Hyperglycemia is associated with reduced testicular function and activin dysregulation in the Ins2Akita+/- mouse model of type 1 diabetes." in: Molecular and cellular endocrinology, Vol. 446, pp. 91-101, (2017) (PubMed).

Target Details for IL-1 beta

Target: IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))

Alternative Name: IL-1b

Background: IL-1β(Interleukin 1 Beta)/IL-1beta/IL1B/IL1-BETA/IL1F2/catabolin

UniProt: P10749

Pathways: NF-kappaB Signaling, Interferon-gamma Pathway, TLR Signaling, Negative Regulation of Hormone Secretion, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Autophagy, Cancer Immune Checkpoints, Inflammasome