ABCC8 ELISA Kit

Catalog No. ABIN5652076

Product Details ABCC8 ELISA Kit

Target: ABCC8 (ATP-Binding Cassette, Sub-Family C (CFTR/MRP), Member 8 (ABCC8))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 1.25 ng/mL - 20 ng/mL

Minimum Detection Limit: 1.25 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of ATP Binding Cassette Transporter C8 (ABCC8). No significant cross-reactivity or interference between ATP Binding Cassette Transporter C8 (ABCC8) and analogues was observed.

Sensitivity: 0.52 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to ATP Binding Cassette Transporter C8 (ABCC8). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to ATP Binding Cassette Transporter C8 (ABCC8). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ATP Binding Cassette Transporter C8 (ABCC8), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of ATP Binding Cassette Transporter C8 (ABCC8) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level ATP Binding Cassette Transporter C8 (ABCC8) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level ATP Binding Cassette Transporter C8 (ABCC8) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for ABCC8

Target: ABCC8 (ATP-Binding Cassette, Sub-Family C (CFTR/MRP), Member 8 (ABCC8))

Alternative Name: ATP Binding Cassette Transporter C8 (ABCC8 Products)

Synonyms: ABC36 ELISA Kit, HHF1 ELISA Kit, HI ELISA Kit, HRINS ELISA Kit, MRP8 ELISA Kit, PHHI ELISA Kit, SUR ELISA Kit, SUR1 ELISA Kit, SUR1delta2 ELISA Kit, TNDM2 ELISA Kit, D930031B21Rik ELISA Kit, Sur ELISA Kit, Sur1 ELISA Kit, ATP binding cassette subfamily C member 8 ELISA Kit, ATP-binding cassette, sub-family C (CFTR/MRP), member 8 ELISA Kit, ABCC8 ELISA Kit, Abcc8 ELISA Kit

Background:

Gene Name: ATP Binding Cassette Transporter C8

Gene Aliases: ABC-C8, PHHI, MRP8, ABC36, HHF1, HI, HRINS, SUR, SUR1, TNDM2, Sulfonylurea Receptor(Hyperinsulinemia)

Gene ID: 6833

UniProt: Q09428

Pathways: Negative Regulation of Hormone Secretion