CYR61 ELISA Kit

Catalog No. ABIN5653465

Product Details CYR61 ELISA Kit

Target: CYR61 (Cysteine-Rich, Angiogenic Inducer, 61 (CYR61))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 62.5 pg/mL - 4000 pg/mL

Minimum Detection Limit: 62.5 pg/mL

Application: ELISA

Sample Type: Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Cysteine Rich Protein, Angiogenic Inducer 61 (CYR61). No significant cross-reactivity or interference between Cysteine Rich Protein, Angiogenic Inducer 61 (CYR61) and analogues was observed.

Sensitivity: 26.1 pg/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cysteine Rich Protein, Angiogenic Inducer 61 (CYR61). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cysteine Rich Protein, Angiogenic Inducer 61 (CYR61). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cysteine Rich Protein, Angiogenic Inducer 61 (CYR61), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cysteine Rich Protein, Angiogenic Inducer 61 (CYR61) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cysteine Rich Protein, Angiogenic Inducer 61 (CYR61) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cysteine Rich Protein, Angiogenic Inducer 61 (CYR61) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for CYR61

Target: CYR61 (Cysteine-Rich, Angiogenic Inducer, 61 (CYR61))

Alternative Name: Cysteine Rich Protein, Angiogenic Inducer 61 (CYR61 Products)

Synonyms: CCN1 ELISA Kit, GIG1 ELISA Kit, IGFBP10 ELISA Kit, ccn1 ELISA Kit, gig1 ELISA Kit, Xcyr61 ELISA Kit, igfbp10 ELISA Kit, MGC69281 ELISA Kit, MGC69539 ELISA Kit, cyr61-l2 ELISA Kit, zgc:158158 ELISA Kit, CYR61 ELISA Kit, AI325051 ELISA Kit, Igfbp10 ELISA Kit, cysteine rich angiogenic inducer 61 ELISA Kit, cysteine-rich, angiogenic inducer, 61 ELISA Kit, cysteine-rich, angiogenic inducer, 61 like 2 ELISA Kit, cysteine rich protein 61 ELISA Kit, cysteine rich angiogenic inducer 61 S homeolog ELISA Kit, CYR61 ELISA Kit, Cyr61 ELISA Kit, cyr61 ELISA Kit, cyr61l2 ELISA Kit, cyr61.S ELISA Kit

Background:

Gene Name: Cysteine Rich Protein, Angiogenic Inducer 61

Gene Aliases: CCN1, GIG1, IGFBP10, CCN family member 1, Insulin-like growth factor-binding protein 10

Gene ID: 3491

UniProt: O00622

Pathways: Positive Regulation of Endopeptidase Activity, Growth Factor Binding