DIO2 ELISA Kit
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- Target See all DIO2 ELISA Kits
- DIO2 (Deiodinase, Iodothyronine, Type II (DIO2))
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Reactivity
- Rat
- Detection Method
- Colorimetric
- Method Type
- Sandwich ELISA
- Detection Range
- 0.781 ng/mL - 50 ng/mL
- Minimum Detection Limit
- 0.781 ng/mL
- Application
- ELISA
- Sample Type
- Tissue Homogenate
- Analytical Method
- Quantitative
- Specificity
- This assay has high sensitivity and excellent specificity for detection of Deiodinase, Iodothyronine, Type II (DIO2). No significant cross-reactivity or interference between Deiodinase, Iodothyronine, Type II (DIO2) and analogues was observed.
- Sensitivity
- 0.24 ng/mL
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- Comment
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- Assay Time
- 3 h
- Plate
- Pre-coated
- Protocol
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Deiodinase, Iodothyronine, Type II (DIO2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Deiodinase, Iodothyronine, Type II (DIO2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Deiodinase, Iodothyronine, Type II (DIO2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Deiodinase, Iodothyronine, Type II (DIO2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- Assay Precision
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Deiodinase, Iodothyronine, Type II (DIO2) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Deiodinase, Iodothyronine, Type II (DIO2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - Restrictions
- For Research Use only
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- Handling Advice
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- Storage
- 4 °C,-20 °C
- Storage Comment
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- Expiry Date
- 4-8 months
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- Target See all DIO2 ELISA Kits
- DIO2 (Deiodinase, Iodothyronine, Type II (DIO2))
- Alternative Name
- Deiodinase, Iodothyronine, Type II (DIO2 Products)
- Synonyms
- 5DII ELISA Kit, D2 ELISA Kit, DIOII ELISA Kit, SelY ELISA Kit, TXDI2 ELISA Kit, AI324267 ELISA Kit, Deio2 ELISA Kit, cb684 ELISA Kit, DIO2 ELISA Kit, dio2-A ELISA Kit, 5dii ELISA Kit, sely ELISA Kit, txdi2 ELISA Kit, iodothyronine deiodinase 2 ELISA Kit, deiodinase, iodothyronine, type II ELISA Kit, deiodinase, iodothyronine, type 2 L homeolog ELISA Kit, deiodinase, iodothyronine, type 2 ELISA Kit, type II iodothyronine deiodinase ELISA Kit, DIO2 ELISA Kit, Dio2 ELISA Kit, dio2 ELISA Kit, dio2.L ELISA Kit, LOC100861220 ELISA Kit
- Background
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Gene Name: Deiodinase, Iodothyronine, Type II
Gene Aliases: 5DII, D2, SelY, TXDI2, Thyroxine Deiodinase,Type II, Type-II 5'-deiodinase
- Gene ID
- 65162
- UniProt
- P70551
- Pathways
- Hormone Transport, Hormone Activity
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