MMP 9 CLIA Kit

Catalog No. ABIN5656795

Product Details MMP 9 CLIA Kit

Target: MMP 9 (MMP9) (Matrix Metallopeptidase 9 (Gelatinase B, 92kDa Gelatinase, 92kDa Type IV Collagenase) (MMP9))

Reactivity: Mouse

Detection Method: Chemiluminescent

Method Type: Sandwich ELISA

Detection Range: 117.19 pg/mL - 30000 pg/mL

Minimum Detection Limit: 117.19 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Matrix Metalloproteinase 9 (MMP9). No significant cross-reactivity or interference between Matrix Metalloproteinase 9 (MMP9) and analogues was observed.

Sensitivity: 14.9 pg/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 2 - 3 h

Plate: Pre-coated

Protocol: The microplate provided in this kit has been pre-coated with an antibody specific to Matrix Metalloproteinase 9 (MMP9). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Matrix Metalloproteinase 9 (MMP9). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Matrix Metalloproteinase 9 (MMP9) level in the sample or standard.,

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Matrix Metalloproteinase 9 (MMP9) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Matrix Metalloproteinase 9 (MMP9) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details

Target: MMP 9 (MMP9) (Matrix Metallopeptidase 9 (Gelatinase B, 92kDa Gelatinase, 92kDa Type IV Collagenase) (MMP9))

Alternative Name: Matrix Metalloproteinase 9 (MMP9 Products)

Synonyms: CLG4B CLIA Kit, GELB CLIA Kit, MANDP2 CLIA Kit, MMP-9 CLIA Kit, AW743869 CLIA Kit, B/MMP9 CLIA Kit, Clg4b CLIA Kit, pro-MMP-9 CLIA Kit, mmp-9 CLIA Kit, fgMMP-9 CLIA Kit, mmp9 CLIA Kit, ZFMMP-9 CLIA Kit, wu:fb02g06 CLIA Kit, wu:fb07b05 CLIA Kit, wu:fi98c09 CLIA Kit, wu:fj05a08 CLIA Kit, zgc:64165 CLIA Kit, clg4b CLIA Kit, gelb CLIA Kit, mandp2 CLIA Kit, matrix metallopeptidase 9 CLIA Kit, collagenase CLIA Kit, matrix metalloproteinase 9 CLIA Kit, matrix metallopeptidase 9 S homeolog CLIA Kit, MMP9 CLIA Kit, RB3913 CLIA Kit, BPSS0666 CLIA Kit, Sbal_3732 CLIA Kit, Bcer98_0486 CLIA Kit, Shew185_0630 CLIA Kit, Shal_3056 CLIA Kit, Sbal195_0657 CLIA Kit, Lbys_3550 CLIA Kit, Palpr_2084 CLIA Kit, Mmp9 CLIA Kit, mmp9 CLIA Kit, mmp9.S CLIA Kit

Background:

Gene Name: Matrix Metalloproteinase 9

Gene Aliases: GELB, Gelatinase B, CLG4B, CLG4-B, 92 KDa Gelatinase, 92 kDa Type IV Collagenase

Gene ID: 17395

UniProt: P41245

Pathways: Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, CXCR4-mediated Signaling Events