WNT3 CLIA Kit
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- Target See all WNT3 CLIA Kits
- WNT3 (Wingless-Type MMTV Integration Site Family, Member 3 (WNT3))
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Reactivity
- Human
- Detection Method
- Chemiluminescent
- Method Type
- Sandwich ELISA
- Detection Range
- 468.75 pg/mL - 30000 pg/mL
- Minimum Detection Limit
- 468.75 pg/mL
- Application
- ELISA
- Sample Type
- Cell Lysate, Plasma, Serum, Tissue Homogenate
- Analytical Method
- Quantitative
- Specificity
- This assay has high sensitivity and excellent specificity for detection of Wingless Type MMTV Integration Site Family, Member 3 (WNT3). No significant cross-reactivity or interference between Wingless Type MMTV Integration Site Family, Member 3 (WNT3) and analogues was observed.
- Sensitivity
- 18.25 pg/mL
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- Comment
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- Assay Time
- 2 - 3 h
- Plate
- Pre-coated
- Protocol
- The microplate provided in this kit has been pre-coated with an antibody specific to Wingless Type MMTV Integration Site Family, Member 3 (WNT3). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Wingless Type MMTV Integration Site Family, Member 3 (WNT3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Wingless Type MMTV Integration Site Family, Member 3 (WNT3) level in the sample or standard.,
- Assay Precision
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Wingless Type MMTV Integration Site Family, Member 3 (WNT3) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Wingless Type MMTV Integration Site Family, Member 3 (WNT3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - Restrictions
- For Research Use only
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- Handling Advice
- Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- Storage
- 4 °C,-20 °C
- Storage Comment
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- Expiry Date
- 4-8 months
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- Target See all WNT3 CLIA Kits
- WNT3 (Wingless-Type MMTV Integration Site Family, Member 3 (WNT3))
- Alternative Name
- Wingless Type MMTV Integration Site Family, Member 3 (WNT3 Products)
- Synonyms
- INT4 CLIA Kit, Ci-Wnt3 CLIA Kit, WNT3 CLIA Kit, int4 CLIA Kit, Xwnt3 CLIA Kit, wnt-3 CLIA Kit, wnt3l CLIA Kit, Xwnt-3 CLIA Kit, wnt3 CLIA Kit, Int-4 CLIA Kit, Wnt-3 CLIA Kit, wnt3-A CLIA Kit, Wnt family member 3 CLIA Kit, wingless-type MMTV integration site family, member 3 CLIA Kit, proto-oncogene Wnt-3 CLIA Kit, wingless-type MMTV integration site family member 3 CLIA Kit, Wnt family member 3A CLIA Kit, wingless-type MMTV integration site family member 3 L homeolog CLIA Kit, WNT3 CLIA Kit, Wnt3 CLIA Kit, wnt3 CLIA Kit, LOC468297 CLIA Kit, WNT3A CLIA Kit, wnt3.L CLIA Kit
- Background
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Gene Name: Wingless Type MMTV Integration Site Family, Member 3
Gene Aliases: INT4, Proto-oncogene Int-4 homolog
- Gene ID
- 7473
- UniProt
- P56703
- Pathways
- WNT Signaling, Regulation of Cell Size
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